Occurrence of patulin in various fruit juices from South Korea: An exposure assessment

Cho, Mi Sook; Kim, Kyeong-Yeol; Seo, Eun‐Kyoung; Kassim, Neema; Mtenga, Adelard B.; Shim, Won Bo; Lee, Soo Hyung; Chung, Donghun

doi:10.1007/s10068-010-0001-6

Cited by 31 publications

(15 citation statements)

References 13 publications

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“…Due to the molecule maximal absorbance at 276 nm, this wavelength is used for patulin identification and quantification purpose (Li et al 2007b;Spadaro et al 2008;Barreira et al 2010;Bonerba et al 2010b;Sargenti and Almeida 2010;Cho et al 2010;Khorrami and Taherkhani 2011;Catana et al 2011;Farhadi and Maleki 2011;Zaied et al 2013;Marsol-Vall et al 2014;Forouzan and Madadlou 2014;Zouaoui et al 2015;Karakose et al 2015;De Clercq et al 2016;Ji et al 2017;Sadok et al 2018;Iqbal et al 2018). Unfortunately, detection based on this wavelength suffers from low selectivity due to obstructing phenolic compounds, especially 5-hydroxymethylfurfural (5-HMF) (structure shown in Fig.…”

Section: Hplc Methods With Uv Absorbance Detectionmentioning

confidence: 99%

“…Thus, it might be a good marker to evaluate thermal damages or aging in food products. 5-HMF is also the most common interfering contaminant observed during mycotoxin determination in apple-derivative samples (Cho et al 2010;Lee et al 2014). Patulin and 5-HMF exhibit strong UV absorption, similar retention time under chromatographic conditions, and consequently tendency to peak overlapping.…”

Section: Hplc Methods With Uv Absorbance Detectionmentioning

confidence: 99%

“…The analysis of reports show frequent occurrence of patulin, although at different level, in apples and various apple-based food commodities from over the World, i.e., Qatar (Hammami et al 2017), Portugal (Barreira et al 2010), Spain (Murillo-Arbizu et al 2009Marsol-Vall et al 2014), Italy (Ritieni 2003;Piemontese et al 2005), Czech Republic (Vaclavikova et al 2015), Romania (Catana et al 2011;Oroian et al 2014), Tunisia (Zaied et al 2013;Zouaoui et al 2015), Brazil (Sargenti and Almeida 2010), Argentina (Funes and Resnik 2009), United States (Harris et al 2009), Iran (Jalali et al 2010;Forouzan and Madadlou 2014), Pakistan (Iqbal et al 2018), South Korea (Cho et al 2010), and Northeast China (Yuan et al 2010). According to published data, patulin concentration in the infected apples could reach 8.8-120.4 mg kg −1 (Piemontese et al 2005;Wu et al 2008;Gaspar and Lucena 2009) (Celli et al 2009).…”

Section: Patulin Occurrence In Fruit Commoditiesmentioning

confidence: 99%

“…The LLE using ethyl acetate followed by the sodium carbonate clean-up is a part of the Association of Official Analytical Chemists (AOAC) official method 2000.02 recommended for apple juice and apple puree preparation for HPLC-UV analysis (MacDonald et al 2000). Recently, this method with or without some modification was applied for the mycotoxin extraction from apple and other fruit juices (Li et al 2007b;Spadaro et al 2008;Barreira et al 2010;Cho et al 2010;Yuan et al 2010;Forouzan and Madadlou 2014;Zouaoui et al 2015;Hammami et al 2017) as well as for solid samples (apples, pears, grapes, pineapples, baby food) (Bonerba et al 2010b;Karakose et al 2015;Iqbal et al 2018). The extraction step is frequently followed by cleanup of the collected organic phase using sodium carbonate (1.5-2.0% w/v) (Li et al 2007b;Bonerba et al 2010b;Cho et al 2010;Yuan et al 2010;Zaied et al 2013;Forouzan and Madadlou 2014;Karakose et al 2015;Zouaoui et al 2015;Iqbal et al 2018) to remove the interfering polyphenols (Marsol-Vall et al 2014).…”

Section: Liquid-liquid Extractionmentioning

confidence: 99%

“…The last step when pH is kept low is the final reconstitution of a sample before analysis. The mobile phase used for sample reconstitution is frequently acidified with acetic acid (Spadaro et al 2008;Funes and Resnik 2009;Barreira et al 2010;Cho et al 2010;Forouzan and Madadlou 2014).…”

Section: Importance Of Sample Ph Controlmentioning

confidence: 99%

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Developments in the Monitoring of Patulin in Fruits Using Liquid Chromatography: an Overview

2018

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Patulin is a mycotoxin that exhibits a number of toxic effects in animals. The main sources of patulin intake in human diet that was shown for EU consumers are apple juice and nectar and for this reason, apple-based food is most often monitored for this mycotoxin. However, the presence of patulin in other fruits, including stone fruits, and soft fruits has been reported as well. Most of them are seasonal, suitable for consumption for short time, and are usually processed in order to be commercially available throughout the year. Patulin can also be generated during food storage and remains stable over food processing procedures. Therefore, constant monitoring of different fruit-based products ought to be carried out to provide proper estimation of human exposure to this toxin. The preferred approach used for mycotoxin determination in a variety of foods is liquid chromatography (LC) allowing quantitative analysis of patulin content. This paper presents recently proposed LC methods with ultraviolet and mass spectrometric detection for patulin quantification in fruits and derived products. We focus on developments in sample preparation and the applied analytical conditions.

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Section: Hplc Methods With Uv Absorbance Detectionmentioning

confidence: 99%

Section: Hplc Methods With Uv Absorbance Detectionmentioning

confidence: 99%

Section: Patulin Occurrence In Fruit Commoditiesmentioning

confidence: 99%

Section: Liquid-liquid Extractionmentioning

confidence: 99%

Section: Importance Of Sample Ph Controlmentioning

confidence: 99%

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Developments in the Monitoring of Patulin in Fruits Using Liquid Chromatography: an Overview

2018

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Fungal mycotoxins

Malysheva

Mavungu

Saeger

2015

Fungal Biomolecules

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An optimized method for the accurate determination of patulin in apple products by isotope dilution-liquid chromatography/mass spectrometry

2015

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Patulin, a mycotoxin produced by several molds in fruits, has been frequently detected in apple products. Therefore, regulatory bodies have established recommended maximum permitted patulin concentrations for each type of apple product. Although several analytical methods have been adopted to determine patulin in food, quality control of patulin analysis is not easy, as reliable certified reference materials (CRMs) are not available. In this study, as a part of a project for developing CRMs for patulin analysis, we developed isotope dilution liquid chromatography-tandem mass spectrometry (ID-LC/MS/MS) as a higher-order reference method for the accurate value-assignment of CRMs. (13)C7-patulin was used as internal standard. Samples were extracted with ethyl acetate to improve recovery. For further sample cleanup with solid-phase extraction (SPE), the HLB SPE cartridge was chosen after comparing with several other types of SPE cartridges. High-performance liquid chromatography was performed on a multimode column for proper retention and separation of highly polar and water-soluble patulin from sample interferences. Sample extracts were analyzed by LC/MS/MS with electrospray ionization in negative ion mode with selected reaction monitoring of patulin and (13)C7-patulin at m/z 153→m/z 109 and m/z 160→m/z 115, respectively. The validity of the method was tested by measuring gravimetrically fortified samples of various apple products. In addition, the repeatability and the reproducibility of the method were tested to evaluate the performance of the method. The method was shown to provide accurate measurements in the 3-40 μg/kg range with a relative expanded uncertainty of around 1%.

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Occurrence of patulin in various fruit juices from South Korea: An exposure assessment

Cited by 31 publications

References 13 publications

Developments in the Monitoring of Patulin in Fruits Using Liquid Chromatography: an Overview

Developments in the Monitoring of Patulin in Fruits Using Liquid Chromatography: an Overview

Fungal mycotoxins

An optimized method for the accurate determination of patulin in apple products by isotope dilution-liquid chromatography/mass spectrometry

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