Occurrence of oleic and 18:1 methyl-branched acyl chains in lipids of Rhodobacter sphaeroides 2.4.1

Granafei, Sara; Losito, Ilario; Salivo, Simona; Tranchida, Peter Q.; Mondello, Luigi; Palmisano, Francesco; Cataldi, Tommaso R. I.

doi:10.1016/j.aca.2015.05.028

Cited by 9 publications

(2 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some detected chains of fatty acids show an odd number of carbon atoms. This fact was already described in the case of other non-sulfurous purple bacteria, such as Rhodopseudomonas acidophila and Rhodobacter (R.) sphaeroides [22][23][24][25][26].…”

Section: Phospholipid Standardsmentioning

confidence: 70%

HPTLC coupled to ESI-Tandem MS for identifying phospholipids associated to membrane proteins in photosynthetic purple bacteria

Lapieza

Jungas

Savirón

et al. 2019

Journal of Liquid Chromatography & Related Technologies

View full text Add to dashboard Cite

High-performance thin-layer chromatography (HPTLC)-densitometry was directly combined with electrospray (ESI) tandem mass spectrometry for obtaining rapid and relevant structural identification of phospholipids (PL) species associated to membrane proteins (MP), in non-sulfur, purple bacteria having photosynthetic activity. Thus, species belonging to phosphatidylcholines (PC), phosphatidylethanolamines (PE), cardiolipins (CL) and phosphatidylglycerols (PG) associated to MP were investigated in bacterial membrane extracts from Rhodobacter (Rb.) blasticus, Rhodospirillum (R.) rubrum and Rhodobaca (Rbc.) bogoriensis, as well as those which are bound to a purified MPphotosynthetic complex from Rbc. bogoriensis. PL-classes were separated using a 7-step gradient-solvent sequence with a previous acid plate preconditioning, using Automated Multiple Development. Band zones of the plate corresponding to PL classes were selected to ensure their direct transfer to ion-trap MS equipment through an elution-based interface. Under the studied conditions, ESI þ-MS spectra of PC and CL mostly showed sodium adducts ([M þ Na] þ) and [M-2H þ 3Na] þ , respectively, when recorded from the plate. The respective sodium adducts were fragmented in the ion-trap, and sodium remained as the charge of the fragment ions, thus being useful for their structural identification through MS/MS. ESI-MS and MS/MS spectra of CL were also obtained as [M-2H] 2À , as well as those of PE and PG species as [M-H]and [M] À , respectively. In this way, relative composition profiles of each studied PL-class by ESI-MS, and further identification of individual PL and the molecular species belonging to each of them by MS/MS were obtained.

show abstract

Section: Phospholipid Standardsmentioning

confidence: 70%

HPTLC coupled to ESI-Tandem MS for identifying phospholipids associated to membrane proteins in photosynthetic purple bacteria

Lapieza

Jungas

Savirón

et al. 2019

Journal of Liquid Chromatography & Related Technologies

View full text Add to dashboard Cite

show abstract

“…A search of the PlantFAdb for unsaturated centrally branched fatty acids in plants did not provide additional examples of monomeric or dimeric centrally branched chain fatty acids . In bacteria and marine fungi, centrally branched monounsaturated fatty acids are more common. − …”

Section: Resultsmentioning

confidence: 99%

PTP1B-Inhibiting Branched-Chain Fatty Acid Dimers from Eremophila oppositifolia subsp. angustifolia Identified by High-Resolution PTP1B Inhibition Profiling and HPLC-PDA-HRMS-SPE-NMR Analysis

et al. 2020

View full text Add to dashboard Cite

Ten new branched-chain fatty acid (BCFA) dimers with a substituted cyclohexene structure, five new monomers, and two known monomers, (2E,4Z,6E)-5-(acetoxymethyl)tetradeca-2,4,6-trienoic acid and its 5-hydroxymethyl analogue, were identified in the leaf extract of Eremophila oppositifolia subsp. angustifolia using a combination of HPLC-PDA-HRMS-SPE-NMR analysis and semipreparative-scale HPLC. The dimers could be classified as three types of Diels−Alder reaction products formed between monomers at two different sites of unsaturation of the dienophile. Two of the monomers represent potential biosynthetic intermediates of branched-chain fatty acids. Several compounds were found by high-resolution bioactivity profiling to inhibit PTP1B and were purified subsequently by semipreparative-scale HPLC. The dimers were generally more potent than the monomers with IC 50 values ranging from 2 to 66 μM, compared to 38−484 μM for the monomers. The ten fatty acid dimers represent both a novel class of compounds and a novel class of PTP1B inhibitors.

show abstract

Gas Chromatography Columns Using Ionic Liquids as Stationary Phase

Talebi

Patil

Armstrong

2020

Green Chemistry and Sustainable Technology

View full text Add to dashboard Cite

Occurrence of oleic and 18:1 methyl-branched acyl chains in lipids of Rhodobacter sphaeroides 2.4.1

Cited by 9 publications

References 55 publications

HPTLC coupled to ESI-Tandem MS for identifying phospholipids associated to membrane proteins in photosynthetic purple bacteria

HPTLC coupled to ESI-Tandem MS for identifying phospholipids associated to membrane proteins in photosynthetic purple bacteria

PTP1B-Inhibiting Branched-Chain Fatty Acid Dimers from Eremophila oppositifolia subsp. angustifolia Identified by High-Resolution PTP1B Inhibition Profiling and HPLC-PDA-HRMS-SPE-NMR Analysis

Gas Chromatography Columns Using Ionic Liquids as Stationary Phase

Contact Info

Product

Resources

About