Occurrence of New Populations and Mating Types of Phytophthora Infestans (Mont) De Bary in Serbia

Ivanovic, Marcela L; Mijatovic, M.; Zecevic, B.; Niepold, F.

doi:10.17660/actahortic.2007.729.85

Cited by 4 publications

(5 citation statements)

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“…The concentration of the sporangia was examined by a haemocytometer and adjusted to 15 × 10 4 sporangia/mL. Prior to inoculation, the suspension was chilled at 4 °C for 2–4 h to encourage zoospore release [ 61 ].…”

Section: Methodsmentioning

confidence: 99%

Leaf Extracts from Resistant Wild Tomato Can Be Used to Control Late Blight (Phytophthora infestans) in the Cultivated Tomato

Arafa

Kamel

Taher

et al. 2022

Plants

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Late blight disease, caused by Phytophthora infestans (Mont.) de Bary, is one of the most challenging diseases threatening tomato production and other Solanaceae crops. Resistance to late blight is found in certain wild species, but the mechanism behind the resistance is not fully understood. The aim of this study was to examine the metabolic profiles in the leaf tissue of late blight-resistant wild tomato and to investigate if leaf extracts from such genotypes could be used to control late blight in tomato production. We included three recognized late blight-resistant wild tomato accessions of Solanum habrochaites (LA1777, LA2855, and LA1352) and two recognized highly susceptible genotypes, S. lycopersicum (‘Super Strain B’) and S. pimpinellifolium (LA0375). The metabolic profiles were obtained in both inoculated and non-inoculated plants by analyzing leaf extracts using high-resolution gas chromatography-mass spectrometry (GC-MS) with three replicate analyses of each genotype. We focused on volatile organic compounds (VOCs) and identified 31 such compounds from the five genotypes with a retention time ranging from 6.6 to 22.8 min. The resistant genotype LA 1777 produced the highest number of VOCs (22 and 21 in the inoculated and control plants, respectively), whereas the susceptible genotype ‘Super Strain B’ produced the lowest number of VOCs (11 and 13 in the respective plants). Among the VOCs, 14 were detected only in the resistant genotypes, while two were detected only in the susceptible ones. In vitro trials, with the use of a detached leaflet assay and whole-plant approach, were conducted. We revealed promising insights regarding late blight management and showed that metabolic profiling may contribute to a better understanding of the mechanisms behind P. infestans resistance in tomato and its wild relatives.

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Section: Methodsmentioning

confidence: 99%

Leaf Extracts from Resistant Wild Tomato Can Be Used to Control Late Blight (Phytophthora infestans) in the Cultivated Tomato

Arafa

Kamel

Taher

et al. 2022

Plants

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“…Inoculum preparation of isolate EG_12 was performed as described [ 15 ]. Prior to artificial inoculation, the suspension was chilled at 4°C for 2–4 h [ 45 ] to allow cleavage of sporangia and release of zoospores.…”

Section: Methodsmentioning

confidence: 99%

Rapid identification of candidate genes for resistance to tomato late blight disease using next-generation sequencing technologies

et al. 2017

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Tomato late blight caused by Phytophthora infestans (Mont.) de Bary, also known as the Irish famine pathogen, is one of the most destructive plant diseases. Wild relatives of tomato possess useful resistance genes against this disease, and could therefore be used in breeding to improve cultivated varieties. In the genome of a wild relative of tomato, Solanum habrochaites accession LA1777, we identified a new quantitative trait locus for resistance against blight caused by an aggressive Egyptian isolate of P. infestans. Using double-digest restriction site–associated DNA sequencing (ddRAD-Seq) technology, we determined 6,514 genome-wide SNP genotypes of an F2 population derived from an interspecific cross. Subsequent association analysis of genotypes and phenotypes of the mapping population revealed that a 6.8 Mb genome region on chromosome 6 was a candidate locus for disease resistance. Whole-genome resequencing analysis revealed that 298 genes in this region potentially had functional differences between the parental lines. Among of them, two genes with missense mutations, Solyc06g071810.1 and Solyc06g083640.3, were considered to be potential candidates for disease resistance. SNP and SSR markers linking to this region can be used in marker-assisted selection in future breeding programs for late blight disease, including introgression of new genetic loci from wild species. In addition, the approach developed in this study provides a model for identification of other genes for attractive agronomical traits.

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“…The isolate was extracted from potato sampled in the region of Guca, Serbia. The isolate belong to A1 mating type according to previous research of Ivanovi et al (2004).…”

Section: P Infestans Isolatementioning

confidence: 99%

“…They have been inoculated with approximately 10 4 sporangia/ml suspension. The suspension has been prepared on rye agar and spent 3 h at 5°C before inoculation (Ivanovi et al, 2004). Inoculated leaves have been incubated for 10 days at 15°C.…”

Section: Inoculationmentioning

confidence: 99%

The level of resistance to late blight Phytophthora infestans (Mont.) de Bary in tomato breeding genotypes in Serbia

Zdravković¹

2011

Afr. J. Agric. Res.

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Late blight (Phytophthora infestans (Mont.) de Bary) was identified as one of the major diseases causing significant economic losses in tomato production. Thus, the development of resistant cultivars and hybrids was included in tomato breeding programs at the Institute for Vegetable Crops, Smederevska Palanka. A line (S-31), Tomato late blight resistance gene Ph-2 carrier, was diallely crossed with four susceptible lines possessing good production characteristics (S-49, S-31, hom-3 and hom-4) in order to obtain resistant high-yield and quality hybrids. Parental lines and five F1 hybrids, as well as three commercially grown susceptible cultivars (Narvik SPF, Adonis, SP-109, negative control) were tested for resistance toward this pathogen. Sensitivity was evaluated ten days after inoculation on the basis of leaf area necrotic spot, using I to VI level scale. The results imply dominant or partially dominant inheritance pattern. Three commercially grown cultivars were susceptible, as expected. Five of F1 hybrids including S-31 and hom-3 parents were resistant to late blight. The best hybrids were S-31 x hom-3 and S-31 x hom-4 when the morphological traits, yield and resistance were taken into account. Obviously, lines from group hom contributed to increased resistance to tomato late blight with respect to resistant parent.

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Occurrence of New Populations and Mating Types of Phytophthora Infestans (Mont) De Bary in Serbia

Cited by 4 publications

References 0 publications

Leaf Extracts from Resistant Wild Tomato Can Be Used to Control Late Blight (Phytophthora infestans) in the Cultivated Tomato

Leaf Extracts from Resistant Wild Tomato Can Be Used to Control Late Blight (Phytophthora infestans) in the Cultivated Tomato

Rapid identification of candidate genes for resistance to tomato late blight disease using next-generation sequencing technologies

The level of resistance to late blight Phytophthora infestans (Mont.) de Bary in tomato breeding genotypes in Serbia

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