Occurrence of mislabeling in meat products using DNA-based assay

Pinto, Angela Di; Bottaro, Marilisa; Bonerba, Elisabetta; Bozzo, Giancarlo; Ceci, Edmondo; Marchetti, Patrizia; Mottola, Anna; Tantillo, Giuseppina

doi:10.1007/s13197-014-1552-y

Cited by 86 publications

(55 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mislabeling of food products has been mentioned as a major public health risk associated with food safety in the food market in 17 of the full-text articles included in this review [68][69][70][71][72][73][74][75][76][77][78][79][80][81][82]154]. Mislabeling of food products includes false advertising, deliberately or accidentally leaving out ingredients, not listing potential health effects, and claiming a food contains ingredients that it does not for financial gain with the intent of deceiving the consumer regarding what is actually in the package [155].…”

Section: Sood M 2014 [67] Indonesia Field Inspectionmentioning

confidence: 99%

Public health risks related to food safety issues in the food market: a systematic literature review

Gizaw

2019

Environ Health Prev Med

199

View full text Add to dashboard Cite

Background: Food safety in the food market is one of the key areas of focus in public health, because it affects people of every age, race, gender, and income level around the world. The local and international food marketing continues to have significant impacts on food safety and health of the public. Food supply chains now cross multiple national borders which increase the internationalization of health risks. This systematic review of literature was, therefore, conducted to identify common public health risks related to food safety issues in the food market. Methods: All published and unpublished quantitative, qualitative, and mixed method studies were searched from electronic databases using a three step searching. Analytical framework was developed using the PICo (population, phenomena of interest, and context) method. The methodological quality of the included studies was assessed using mixed methods appraisal tool (MMAT) version 2018. The included full-text articles were qualitatively analyzed using emergent thematic analysis approach to identify key concepts and coded them into related non-mutually exclusive themes. We then synthesized each theme by comparing the discussion and conclusion of the included articles. Emergent themes were identified based on meticulous and systematic reading. Coding and interpreting the data were refined during analysis.Results: The analysis of 81 full-text articles resulted in seven common public health risks related with food safety in the food market. Microbial contamination of foods, chemical contamination of foods, food adulteration, misuse of food additives, mislabeling, genetically modified foods (GM foods), and outdated foods or foods past their use-by dates were the identified food safety-related public health risks in the food market. Conclusion: This systematic literature review identified common food safety-related public health risks in the food market. The results imply that the local and international food marketing continues to have significant impacts on health of the public. The food market increases internationalization of health risks as the food supply chains cross multiple national borders. Therefore, effective national risk-based food control systems are essential to protect the health and safety of the public. Countries need also assure the safety and quality of their foods entering international trade and ensure that imported foods conform to national requirements.

show abstract

Section: Sood M 2014 [67] Indonesia Field Inspectionmentioning

confidence: 99%

Public health risks related to food safety issues in the food market: a systematic literature review

Gizaw

2019

Environ Health Prev Med

199

View full text Add to dashboard Cite

show abstract

“…Amplification was conducted on a cytochrome b gene with a common forward primer and specific reverse primers, as first described previous literature [18]. The size calculated from these samples were different than previously reported [13], whereas bovine samples, a band size of 320 bp was calculated, and from porcine samples a band size of 360 bp was calculated. Based on this study, conventional and isolation kit method showed a 92% PCR amplification from all samples.…”

Section: Pcr Amplification Showed Varied Results Depending On Dna Isomentioning

confidence: 99%

“…The oligonucleotide primers [13], were synthesized by Integrated DNA Technology, Singapore. According to this study [13], oligonucleotides used were forward primer SIM 5'-GAC CTC CCA GCT CCA TCA AAC ATC TCA TCT TGA TGA AA -3', used for both porcine and bovine samples, and a bovine reverse primer 5'-CTA GAA AAG TGT AAG ACC CGT AAT ATA AG -3' and porcine reverse primer 5'-GCT GAT AGT AGA TTT GTG ATG ACC GTA-3'.…”

Section: Dna Amplification Methodsmentioning

confidence: 99%

“…According to this study [13], oligonucleotides used were forward primer SIM 5'-GAC CTC CCA GCT CCA TCA AAC ATC TCA TCT TGA TGA AA -3', used for both porcine and bovine samples, and a bovine reverse primer 5'-CTA GAA AAG TGT AAG ACC CGT AAT ATA AG -3' and porcine reverse primer 5'-GCT GAT AGT AGA TTT GTG ATG ACC GTA-3'. The amplicon length for porcine samples and bovine samples would be 398 bp and 274 bp, respectively.…”

Section: Dna Amplification Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of DNA Extraction Methods Between Conventional, Kit, Alkali and Buffer-Only for PCR Amplification on Raw and Boiled Bovine and Porcine Meat

Yahya

Firmansyah

Arlisyah³

et al. 2017

JELS

View full text Add to dashboard Cite

Detection of porcine contamination in food material by employing PCR techniques is integral in halal food confirmation. However, PCR is both costly and laborious, particularly in DNA isolation method. This study explores several different methods in DNA extraction for PCR amplification in bovine and porcine raw and boiled meat samples. Four methods for DNA extraction (conventional PCI method, DNA isolation kit, alkaline-based method, and a DNA lysis buffer-only from the same kit) was employed followed by PCR using primers from previous studies and compared for DNA quality and quantity (in six replicates) and PCR amplification on the best three DNA samples. This study shows that in all samples, the conventional method had the best DNA yield based on nanodrop measurement, followed by an alkali-based method, buffer-only method, and DNA isolation kit. Each method except lysis-buffer only had at least one sample with good DNA quality. Conventional and isolation kit showed reliable positive PCR detection for all porcine and bovine samples (92% positive). Using the alkaline-lysis method, DNA was amplified reliably on boiled meat samples (83% positive). Lysis-buffer-only method did not show consistent PCR amplification on the samples used (50% positive). The conclusion was that conventional PCI method and DNA isolation kit showed high reliability in PCR amplification of bovine and porcine meats, both raw and boiled. While high DNA yield was obtained using the alkaline-lysis method, PCR amplification was only successful on boiled samples. Lysis-buffer only method yielded in poor DNA quality and was not able to result in reliable DNA amplification.

show abstract

“…The presence of undeclared species may cause health problems such as food allergy (Naaum et al 2018;Temisak et al 2019) and a fatal neurodegenerative disease caused by bovine spongiform encephalopathy (Sultan et al 2004). The previous studies reviewed that over 50% of processed meat products tested were mislabeled (Cawthorn et al 2013;Di Pinto et al 2015;Quinto et al 2016). Therefore, accurate methods for determination of meat species in food products are required.…”

Section: Introductionmentioning

confidence: 99%

Accurate determination of meat mass fractions using DNA measurements for quantifying meat adulteration

Temisak¹,

Thangsunan²,

Boonil³

et al. 2020

Preprint

View full text Add to dashboard Cite

The problem in meat adulteration and food fraud emphasised the requirement of developing accurate analytical approaches for the quantitative detection in helping the control of meat adulteration. In this study, the droplet digital Polymerase Chain Reaction (ddPCR) assays to quantify the ratios of pork DNA to the total amount of meat DNA were developed by challenging against DNA extracted from a range of gravimetrically prepared matrices of pork in beef. A single copy nuclear DNA gene, β-actin, was employed as a target gene, accompanied with myostatin gene as a cross species target for mammal and poultry meat background in order to quantifying approach. All the developed assays, singleplex, duplex and triplex did not show significant difference in quantification of pork content in beef background and demonstrated a good and comparable performance to the mass fractions. The singleplex assay provided more biases than the other two assays when performing with a low concentration of target species. The duplex assay provided a simultaneous quantification of pork and myostatin, whereas the triplex assay was able to detect pork, beef and myostatin with a decrease of technical error, cost and running time. All proposed methods allowed us to quantify pork addition in beef with a limit of quantification (LOQ) estimated at 0.1% (w/w) and a limit of detection (LOD) down to 0.01% (w/w). The developed triplex assay was also tested with commercial processed foods and showed the ability to determine not only the presence of particular pork or beef but also the quantitative purpose directly without standard curves. Hence, the developed ddPCR assays demonstrated a good trueness and precision of the methods in quantifying pork or beef content for meat adulteration. It is expected that these developed approaches can be applied to help regulators to confidently enforce food labelling obligations.

show abstract

Occurrence of mislabeling in meat products using DNA-based assay

Cited by 86 publications

References 26 publications

Public health risks related to food safety issues in the food market: a systematic literature review

Public health risks related to food safety issues in the food market: a systematic literature review

Comparison of DNA Extraction Methods Between Conventional, Kit, Alkali and Buffer-Only for PCR Amplification on Raw and Boiled Bovine and Porcine Meat

Accurate determination of meat mass fractions using DNA measurements for quantifying meat adulteration

Contact Info

Product

Resources

About