Occurrence of Melampsora rusts in biomass willow plantations for renewable energy in the United Kingdom

Pei, M. H.; Hunter, T.; Ruiz, C.

doi:10.1016/s0961-9534(99)00038-0

Cited by 34 publications

(37 citation statements)

References 9 publications

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“…This is a prerequisite for resolution of these phylogenetic and pathological studies. Additionally pathogenicity tests using leaf disc techniques (Pei et al, 1999) have to be realised on a range of willow clones from different species. Therefore, the multiplication of selected willow clones was necessary as the basis for a rust screening program.…”

Section: Introductionmentioning

confidence: 99%

Approaches on vegetative propagation of difficult-to-root Salix caprea

Liesebach¹,

Naujoks²

2004

Plant Cell, Tissue and Organ Culture

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In the framework of a willow rust research project, it was necessary to include vegetatively propagated plant material of selected sallow trees (Salix caprea L.) into biotests for identification of pathotypes. Since it was not possible to root sufficient clonal plants by conventional cutting propagation, the applicability of tissue culture methods was tested. From 10 selected donor trees of Salix caprea newly sprouted shoots were harvested and transferred to nutrient media after surface disinfection. The cultures were grown at 20-22°C, illuminated with warm-white fluorescent tubes. The majority of shoot tips and nodal segments died during the first month, but only with nursery-grown plants this was caused by bacteria contamination. Two clones could be established easily on hormone-free medium. Five clones could be initiated only after repeated subcultures on various media variants. Three clones failed completely. Different basic media compositions were tested and Woody Plant Medium, supplemented with 0.1% activated charcoal, proved to be best for most of the sallow clones. Well developed rooted plantlets were used in vitro for microcutting propagation. The resulting plants were transferred to soil and could be included in the rust screening program after acclimatising. The applicability of micropropagation for selected Salix caprea donor trees was strongly depending on the genotype. But the comparison of results from microcuttings with conventional cutting propagation showed that these methods were successful for different clones each.

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Section: Introductionmentioning

confidence: 99%

Approaches on vegetative propagation of difficult-to-root Salix caprea

Liesebach¹,

Naujoks²

2004

Plant Cell, Tissue and Organ Culture

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“…In parallel, research on the population biology and genetics of rust initiated at LARS is being continued (Pei et al 1996(Pei et al , 1999(Pei et al , 2002(Pei et al a,b, 2003. Pest studies have concentrated on the blue (Phratora vulgatissima) and brassy (P. vitellinae) willow beetles as these are the most important invertebrate pests of SRC willows in the United Kingdom, and microsatellites were developed for both species (Batley et al 1998Karp and Peacock 2004;Peacock et al 2003Peacock et al , 2004.…”

Section: Global Research Programsmentioning

confidence: 99%

Salix: Botany and Global Horticulture

Kuzovkina

Weih

Romero³

et al. 2007

Horticultural Reviews

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“…After inoculation the leaf discs were incubated in a growth chamber at 16 ° C with 16 h day − 1 illumination at an intensity of 80 µ E m − 2 s − 1 . Inoculum densities (viable spores per leaf disc) were determined by examining the number of Pei et al (1999). Pei et al (1996).…”

Section: Incubation Periodmentioning

confidence: 99%

Quantitative relationships between inoculum of Melampsora larici‐epitea and corresponding disease on Salix

Pei¹,

Ruiz²,

Hunter³

et al. 2002

Plant Pathology

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Six Salix clones were inoculated with urediniospores of four isolates of Melampsora larici-epitea at five inoculum levels using a leaf-disc method. Disease reactions were recorded using a digital camera; the number and size of uredinia were examined using image analysis software; and spore yield per leaf disc was measured. In three Salix / Melampsora combinations, S. × mollissima 'Q83'/Q1 (LET4); S. viminalis '78183'/ V1 (LET1); and S. × stipularis / V1, pustule numbers increased as inoculum density became higher. In the remainder, S. viminalis 'Mullatin'/ V1; S. × calodendron /DB (LET3); and S. burjatica 'Korso'/ K (LR1), pustule numbers initially increased, then decreased as inoculum densities exceeded 140 -360 spores per disc. Calculated infection efficiency ranged from 0·11 to 0·20 on the three willows inoculated with V1: 0·16 -0·68 for S. × calodendron / DB; 0·20 -0·55 for 'Q83'/Q1; and 0·07 -0·48 for Korso / K. In single-spore inoculations, up to 10% of spores produced single uredinia. Infection efficiency increased sharply between inoculum densities of 1-40 spores per leaf disc. Spore yield was more closely correlated to pustule area (accounting for 61·2% variance for the combined data) than to the number of pustules (42·7% variance). For spore yields in relation to pustule numbers, clone-specific individual lines having different intercepts and slopes fitted significantly better than either a single line for all the tested willows, or parallel lines fitted to each clone ( P < 0·001). For spore yields in relation to pustule area, clonespecific individual parallel lines were significantly better than a single line ( P < 0·001).

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Occurrence of Melampsora rusts in biomass willow plantations for renewable energy in the United Kingdom

Cited by 34 publications

References 9 publications

Approaches on vegetative propagation of difficult-to-root Salix caprea

Approaches on vegetative propagation of difficult-to-root Salix caprea

Salix: Botany and Global Horticulture

Quantitative relationships between inoculum of Melampsora larici‐epitea and corresponding disease on Salix

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