Occurrence of iris yellow spot virus from onion crops in Egypt

Abstract

Iris yellow spot virus (IYSV) is a infects onion bulb and seed crops in many countries including Egypt. Results of the mechanical inoculation reveled that, small chlorotic lesions and systemic necrosis were observed on both Nicotiana benthamiana and Datura stramonium after 10 days, while there were no symptoms were appeared on the onion plant. The viral biological transmission with Thrips tabaci was highly reported to be efficiently for virus transmitted. Our results confirmed the presentence of viruslike particles of a Tospovirus infected onion leaf using transmission electron microscopy. Both of sequence and phylogenetic analysis of N gene revealed that our viral isolate is IYSV with 95 % identity with reported Israel isolate. The sequence of N gene had three motifs: casein kinase II Phosphorylation site, N-myristoylation site and protein kinase C phosphorylation site. These motifs are involved in regulation, activity and stability of IYSV. To our knowledge, this is the first molecular characterization of IYSV in Egypt.

The Phytochemical, Antifungal, and First Report of the Antiviral Properties of Egyptian Haplophyllum tuberculatum Extract

“…In the protective assay, the upper-right halves of the leaves were treated with H. tuberculatum WPE 24 h before mechanical viral inoculation. In contrast, the upper-left halves of the leaves were inoculated with TMV only without any treatment [ 54 , 55 ]. Mock leaves treated with a mixture of equal volumes of DMSO, sterile distilled H 2 O, and phosphate buffer with carborundum were used as the controls.…”

Green Synthesized ZnO Nanoparticles Mediated by Mentha Spicata Extract Induce Plant Systemic Resistance against Tobacco Mosaic Virus

“…Under insect-proof greenhouse conditions, tomato seeds were surface sterilized and cultivated in plastic pots (20 cm in diameter) filled with sterilized soil (clay: sand, 1:1). At 28th days post seedlings, tomato seedlings were transplanted into new pots, and one week later, two upper true leaves of each tomato plant were dusted with carborundum and mechanically inoculated with 1 mL of semi-purified TMV as previously described [43,44]. The experiment was carried out in five treatments, each treatment was comprised of five replicates, and each pot contained three tomato plants.…”

Development of a sensitive Luminex xMAP-based microsphere immunoassay for specific detection of Iris yellow spot virus

“…1 and Table 1 ). The amino acid sequences of N protein from different IYSV isolates have high similarity, and thus the IYSV N protein is relatively conserved [ 12 , 31 , 32 ]. With these antibodies, we developed the TAS-ELISA and MIA methods for detecting IYSV.…”

“…IYSV was firstly recorded in onion crops in Idaho, USA in 1989 [ 4 ] and then in the Netherlands in 1992, where it was characterized to be a new, distinct orthotospovirus from Iris hollandica [ 5 ]. Subsequently, IYSV was found to occur in [ 6 ] Brazil [ 7 ], Australia [ 8 ], Japan [ 9 ], Chile [ 10 ], Spain [ 11 ], Guatemala [ 12 ], Peru [ 13 ], India [ 14 ], and Egypt [ 15 ].…”