Occurrence of<i>Schistosoma bovis</i>on Pemba Island, Zanzibar: implications for urogenital schistosomiasis transmission monitoring

Pennance, Tom; Shaali, M.; Amour, Armour Khamis; Suleiman, Khamis Rashid; Allan, Fiona; Rollinson, David; Webster, Bonnie L.

doi:10.1017/s0031182018001154

Cited by 20 publications

(38 citation statements)

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“…The B. globosus with patent S. haematobium (n = 2) and S. bovis (n = 5) infections showed the expected triple banding pattern (snail, trematode, and Schistosoma amplicons, results not shown), and following gel extraction and sequencing the data matched those from the cercariae collected from these samples (GenBank Accessions: MH014047 and MH014044, see [6]).…”

Section: Specificity Testingmentioning

confidence: 79%

“…Arrows highlight the presence of the ~1000 bp trematode band when present (n = 8). B. globosus with a patent S. haematobium infection (Cham10.1 see [6]) was run as a positive control (+ve) and also represented the amplicon profile obtained for the seven patent B. globosus snails (five and two with S. bovis and S. haematobium infections, respectively (see Section 2.4). -ve = the non-template negative control.…”

Section: Testing On Field Samplesmentioning

confidence: 99%

“…As part of a longitudinal xenomonitoring project on Pemba in relation to urogenital schistosomiasis transmission [6], the 'wild-caught' B. globosus and B. nasutus field isolates were available for further validation of the MIX assay. Individual snails had been collected during malacological surveys, individually checked for patent trematode infections by cercarial shedding, and then preserved in 100% ethanol for molecular analysis [6]. Cercariae from infected B. globosus were preserved on Whatman FTA cards and identified using molecular methods as S. haematobium or S. bovis from two and five snails, respectively [6].…”

Section: Specificity Testing and Validation On Field Samplesmentioning

confidence: 99%

“…Bovine, ovine, and caprine schistosomiasis is also of significant veterinary and economic importance across sub-Saharan Africa [4,5] and is caused by infection of cattle, sheep, and goats with species closely related to S. haematobium (termed S. haematobium group species), primarily Schistosoma bovis, Schistosoma curassoni, and Schistosoma mattheei. Overlapping geographical distribution of multiple schistosome and intermediate snail host species strains complicates disease transmission surveillance in (co)endemic zones [2,6,7].…”

Section: Introductionmentioning

confidence: 99%

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Development of a Molecular Snail Xenomonitoring Assay to Detect Schistosoma haematobium and Schistosoma bovis Infections in their Bulinus Snail Hosts

et al. 2020

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Schistosomiasis, a neglected tropical disease of medical and veterinary importance, transmitted through specific freshwater snail intermediate hosts, is targeted for elimination in several endemic regions in sub-Saharan Africa. Multi-disciplinary methods are required for both human and environmental diagnostics to certify schistosomiasis elimination when eventually reached. Molecular xenomonitoring protocols, a DNA-based detection method for screening disease vectors, have been developed and trialed for parasites transmitted by hematophagous insects, such as filarial worms and trypanosomes, yet few have been extensively trialed or proven reliable for the intermediate host snails transmitting schistosomes. Here, previously published universal and Schistosoma-specific internal transcribed spacer (ITS) rDNA primers were adapted into a triplex PCR primer assay that allowed for simple, robust, and rapid detection of Schistosoma haematobium and Schistosoma bovis in Bulinus snails. We showed this two-step protocol could sensitively detect DNA of a single larval schistosome from experimentally infected snails and demonstrate its functionality for detecting S. haematobium infections in wild-caught snails from Zanzibar. Such surveillance tools are a necessity for succeeding in and certifying the 2030 control and elimination goals set by the World Health Organization.

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Section: Specificity Testingmentioning

confidence: 79%

Section: Testing On Field Samplesmentioning

confidence: 99%

Section: Specificity Testing and Validation On Field Samplesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of a Molecular Snail Xenomonitoring Assay to Detect Schistosoma haematobium and Schistosoma bovis Infections in their Bulinus Snail Hosts

et al. 2020

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“…With more careful sampling of miracidia obtained from infected patients, Séne et al (2018) revealed many hitherto unknown, fine scale heterogeneities in transmission of schistosomes between humans and livestock. Further surprises in the transmission of schistosomiasis on Zanzibar are evidenced by Pennance et al (2018); their careful molecular characterization of schistosome cercariae shed from infected Bulinus on Pemba confirmed autochthonous transmission of S. bovis (Pennance et al, 2018).…”

Section: Parasitology 1643mentioning

confidence: 98%

Advancing the multi-disciplinarity of parasitology within the British Society for Parasitology: studies of host–parasite evolution in an ever-changing world

et al. 2018

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The study of parasites typically crosses into other research disciplines and spans across diverse scales, from molecular- to populational-levels, notwithstanding promoting an understanding of parasites set within evolutionary time. Today, the 2030 Sustainable Development Goals (SDGs) help frame much of contemporary parasitological research, since parasites can be found in all ecosystems, blighting human, animal and plant health. In recognition of the multi-disciplinary nature of parasitological research, the 2017 Autumn Symposium of the British Society for Parasitology was held in London to provide a forum for novel exchange across medical, veterinary and wildlife fields of study. Whilst the meeting was devoted to the topic of parasitism, it sought to foster mutualism, the antithesis perhaps of parasitism, by forging new academic connections and social networks to exchange novel ideas. The meeting also celebrated the longstanding career of Professor David Rollinson, FLS in the award of the International Federation for Tropical Medicine Medal for his efforts spanning 40 years of parasitological research. Indeed, David has done so much to explore and promote the fascinating biology of parasitism, as exemplified by the 15 manuscripts contained within this Special Issue.

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Assessment of schistosomiasis transmission in the River Nile at Greater Cairo using malacological surveys and cercariometry

et al. 2022

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Continuous eld studies on the abundance and distribution of freshwater snails and cercarial populations are important for schistosomiasis control programs. In the present work, snail surveys and cercariometry were conducted for four successive seasons at 12 sites on the Nile River banks in the area of Greater Cairo to identify potential transmission foci for schistosomiasis. In addition, water physicochemical parameters were recorded. The results showed that the electrical conductivity, total dissolved solids, dissolved oxygen, and pH were within the permissible levels, except that the water temperature showed an increase, especially in the spring season. Malacological surveys identi ed 10 native snail species at the studied sites of the Nile River, namely Bulinus truncatus, Biomphalaria alexandrina, Lymnaea natalensis, Lanistes carinatus, Cleopatra bulimoides, Melanoides tuberculata, Helisoma duryi, Bellamya unicolor, Physa acuta, Thedoxus niloticus, and one invasive snail species, Thiara scabra. The calculated diversity index indicated that the structure of snails' habitats was poor, while Evenness index indicated that the individuals were not distributed equally. Results of natural infection identi ed no schistosome cercariae in B. truncatus and B. alexandrina. However, results of the cercariometry recovered Schistosoma cercariae in all the surveyed sites during all seasons with variable distribution. The preceding data suggest that, there are still some active transmission foci for schistosomiasis infection in the Nile River. Moreover, the present nding highlights the importance of cercariomety as a complementary approach to snail samplings for the identi cation of transmission foci for schistosomiasis.

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Occurrence ofSchistosoma bovison Pemba Island, Zanzibar: implications for urogenital schistosomiasis transmission monitoring

Cited by 20 publications

References 50 publications

Development of a Molecular Snail Xenomonitoring Assay to Detect Schistosoma haematobium and Schistosoma bovis Infections in their Bulinus Snail Hosts

Development of a Molecular Snail Xenomonitoring Assay to Detect Schistosoma haematobium and Schistosoma bovis Infections in their Bulinus Snail Hosts

Advancing the multi-disciplinarity of parasitology within the British Society for Parasitology: studies of host–parasite evolution in an ever-changing world

Assessment of schistosomiasis transmission in the River Nile at Greater Cairo using malacological surveys and cercariometry

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