Occurrence of Cucumber vein yellowing virus in cucurbitaceous species in southern Portugal

Host Range Studies for Tomato chlorosis virus, and Cucumber vein yellowing virus Transmitted by Bemisia tabaci (Gennadius)

“…Additionally all sequences shared 99% sequence identity with GenBank accession number AY290865-CP sequence, 99% identity with accession number AJ301640-CP sequence and 96% identity with accession number AF233429-CP sequence. A Spanish isolate of CVYV was used in this study and the sequence identity found was in agreement with that previously reported by Louro et al (2004) for a CVYV isolate from cucumber in Portugal, which also utilised the primers of Cuadrado et al (2001) and reported 95.6% identity to the sequence published for a CVYV isolate from Israel AF233429-CP sequence, and 99% identity with an isolate from Spain, AY290865.…”

“…The consensus sequences for all amplicons obtained were 445 bp in length, 4 bp smaller than that reported by Louro et al (2004) due to the sequencing reaction terminating this number of bases shorter than that reported. 100% homology was found for all sequenced amplicons both with each other as expected since one CVYV isolate was employed, and with the CVYV capsid protein (CP) sequence recorded on the GenBank accession number AY424869, (Louro et al, 2004). Additionally all sequences shared 99% sequence identity with GenBank accession number AY290865-CP sequence, 99% identity with accession number AJ301640-CP sequence and 96% identity with accession number AF233429-CP sequence.…”

“…CVYV causes severe disease of cucumbers and other cucurbits in the eastern Mediterranean basin (Lecoq et al, 2000), and considerable losses in cucumber infected with CVYV have been reported (Cuadrado et al, 2001). Symptoms reported on naturally infected cucurbits in southern Portugal (Louro et al, 2004) comprise vein yellowing, stunting and sudden plant death in protected melons (Cucumis melo), mild leaf chlorosis and split fruits with internal necrosis in watermelons (Citrullus lanatus) and vein clearing and mottling on leaves of cucumbers (C. sativus) and squashes (Cucurbita pepo). CVYV has been recorded in Spain, Israel, Jordan, Turkey (Jones, 2003), and southern Portugal (Louro et al, 2004) and included on the EPPO A2 list.…”

“…Symptoms reported on naturally infected cucurbits in southern Portugal (Louro et al, 2004) comprise vein yellowing, stunting and sudden plant death in protected melons (Cucumis melo), mild leaf chlorosis and split fruits with internal necrosis in watermelons (Citrullus lanatus) and vein clearing and mottling on leaves of cucumbers (C. sativus) and squashes (Cucurbita pepo). CVYV has been recorded in Spain, Israel, Jordan, Turkey (Jones, 2003), and southern Portugal (Louro et al, 2004) and included on the EPPO A2 list. CVYV-Isr and CVYV-Jor are two recognised strains of CVYV from Israel and Jordan respectively (Lecoq et al, 2000).…”

Low genetic diversity among Cucumber vein yellowing virus isolates from Spain

“…Although CVYV was reported only in the Middle East for many years [10,13,14], it was found for the first time in cucumber crops in Spain during autumn of 2000 [15] and readily spread into cucurbitaceous crops throughout the area during spring of 2001 [16,17]. In addition, the virus reached neighboring Portugal in 2003 and has been also recently described in Cyprus [18,19].…”