Occurrence of Helicobacter Pylori in Specimens of Chronic Gastritis and Gastric Adenocarcinoma Patients: A Retrospective Study at University Teaching Hospital, Kigali, Rwanda

Abstract

Introduction: Helicobacter pylori (H. pylori) infection is the major cause of gastroduodenal diseases in populations of different ages.We conducted aretrospective studyusing archived tissue samples to determine the prevalence of H. pylori infection among patients diagnosed with gastritis and gastric adenocarcinoma by histopathology cases in one hospital in Rwanda. Materials and methods: Cases of chronic gastritis and gastric adenocarcinoma histologically diagnosed in a tertiary hospital in Rwanda over the period of 2016-2018 were studied for the presence of H. pylori using immunohistochemistry. Diagnosis of positive cases considered immunoreactivity as well as bacterial morphology, including spiral, rod-shaped, angulated and coccoid forms. Results: Three hundred and seven cases were included in this study; chronic gastritis and gastric adenocarcinoma representing 39% and 61%, respectively. The overall frequency of H. pylori infection was 77.5% (80% among chronic gastritis cases versus 76% among gastric adenocarcinoma cases). Prevalence of H. pylori infection in chronic gastritis and adenocarcinoma did not significantly associate with age and sex. Conclusion: The prevalence of H. pylori was high among chronic gastritis and gastric adenocarcinoma cases in Rwanda. Pathologists should investigate the presence of H. pylori in gastric biopsies. Our data shows immunohistochemistry method is feasible and adequate to facilitate detection of H. pylori, which may guide timely treatment.

Awareness, attitude, and practice of pediatricians in relation to helicobacter pylori infection, diagnosis, and management in Rwanda

“…Regardless, the key factors that were reported to increase the risk for HPI in both HIC and LMIC were often correlated with the socioeconomic status of the households, overcrowding in housing, the number of people in the family, and older siblings [8,9]. In Rwanda, it was found that 80% of adult patients aged between 15 and 92 years with gastritis at CHUK from 2016 to 2018 [10]. Two approved methods for diagnosing HPI are usually categorized as non-invasive, minimally invasive, or invasive methods with upper gastro-intestinal endoscopy with a sample for biopsy [11].…”

Utilidad de la coloración de Giemsa para diagnosticar Helicobacter pylori en pacientes con lesiones preneoplásicas

“…Los segundos están basados en la realización de endoscopia digestiva superior (EDS) con toma de biopsias e incluyen la prueba de la ureasa rápida (S = 80%-95% y E = 95%-100%), el cultivo microbiológico (S = 70% y E = 100%), las pruebas moleculares (S = 91% y E = 100%) e histopatología (S = 90%-95% y E = 95%-98%) (11) . Esta última tiene varias ventajas porque permite el diagnóstico de la infección, determina el grado de inflamación de la mucosa gástrica y permite evaluar la presencia de LPN (12) .…”