Occurrence of Haemolytic Escherichia Coli Serogroups and Their Relationship to Pig Diseases in Queensland

Abstract

Summary Serological examination of 177 strains of haemolytic Escherichia coil from pigs from 95 piggeries showed that 50 isolates from 28 piggeries were agglutinated by serums prepared against 0141:K85ab, 45 from 20 piggeries by 0141:K85ac, 32 isolates from 19 piggeries by 08 and four isolates from two piggeries by 0139:K82. Forty‐six strains from 26 piggeries were unclassified. Ninety strains were isolated from intestine and/or mesenteric lymph nodes and 87 from other organs. Of the 95 outbreaks associated with haemolytic E. coli, 41 were diagnosed as oedema disease, 40 as coli‐enteritis and the remainder were miscellaneous conditions. The clinical and pathological features of outbreaks associated with colibacillosis and septicaemic salmonellosis were compared and discussed.

Studies on Escherichia coli in Pigs II. Serological Investigations1)

“…SODERLIND Distribution of E. coli strains in different organs and herds From a large number of pigs E. coli strains belonging to the same 0-group were found in the intestinal tract and in other internal organs. These findings are in agreement with the reports of several other authors, namely that the isolation of E. coli strains from both the intestinal tract and other internal organs is common (MANSSON, 1962;SCHULMAN et al, 1965;ELDER et al, 1969;SHREEVE and THOMLINSON, 1970). Contrary to this, others (GORDON and LUKE, 1958;SMITH and JONES, 1963;WIT-TIG, 1965, CALLEAR andSMITH, 1966;MOON et al, 1966) generally found the growth of E. coli bacteria only in the intestinal tract.…”

“…1967; SWEENEY, 1968;ELDER et al, 1969;KOSTAKIEV and DANCHEV, 1969;BERTSCHINGER, 1970;KNOX and DAM, 1970; have studied only haemolytic E. coli strains. In material A, non-haemolytic E. coli strains belonging to one 0-group were isolated from the intestinal tract and other internal organs in 4 pigs, all of them belonging to age-group I.…”

Rapid identification of virulence genes in enterotoxigenic Escherichia coli isolates associated with diarrhoea in Queensland piggeries

“…Surprisingly, there are few reports of which ETEC pathotypes and serotypes are present in Queensland piggeries, although early studies indicated the predominance of serotypes O8, O149 and O141 in both ND and PWD. 1,17,18 Early studies focused on the identification of fimbriae and enterotoxins by the use of serological tests and biological assays. 19 When applied to large-scale studies, these methods can be time consuming, expensive, depend very much on the expression of virulence factors on synthetic media, and in some cases require euthanasia of animals.…”