Occurrence of grapevine trunk diseases affecting the native cultivar Pedro Ximénez in southern Spain

Agustí-Brisach, Carlos; López-Moral, Ana; Raya-Ortega, Ma Carmen; Franco, Rosa; Roca-Castillo, Luis F; Trapero, A.

doi:10.1007/s10658-018-1585-6

Cited by 16 publications

(6 citation statements)

References 47 publications

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“…In general, black-foot, Petri, and Botryosphaeria dieback are the most common GTDs affecting young vineyards, whereas Esca (in the strict sense) and Eutypa dieback are the typical GTDs affecting old vineyards. In this second case, Petri disease (Esca complex) and Botryosphaeria dieback can also frequently occur [9].…”

Section: Introductionmentioning

confidence: 81%

Untargeted and Targeted LC-MS/MS Based Metabolomics Study on In Vitro Culture of Phaeoacremonium Species

Reveglia

Raimondo

Cimmino³

et al. 2022

JoF

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Grapevine (Vitis vinifera L.) can be affected by many different biotic agents, including tracheomycotic fungi such as Phaeomoniella chlamydospora and Phaeoacremonium minimum, which are the main causal agent of Esca and Petri diseases. Both fungi produce phytotoxic naphthalenone polyketides, namely scytalone and isosclerone, that are related to symptom development. The main objective of this study was to investigate the secondary metabolites produced by three Phaeoacremonium species and to assess their phytotoxicity by in vitro bioassay. To this aim, untargeted and targeted LC-MS/MS-based metabolomics were performed. High resolution mass spectrometer UHPLC-Orbitrap was used for the untargeted profiling and dereplication of secondary metabolites. A sensitive multi reaction monitoring (MRM) method for the absolute quantification of scytalone and isosclerone was developed on a UPLC-QTrap. Different isolates of P. italicum, P. alvesii and P. rubrigenum were grown in vitro and the culture filtrates and organic extracts were assayed for phytotoxicity. The toxic effects varied within and among fungal isolates. Isosclerone and scytalone were dereplicated by matching retention times and HRMS and MS/MS data with pure standards. The amount of scytalone and isosclerone differed within and among fungal species. To our best knowledge, this is the first study that applies an approach of LC-MS/MS-based metabolomics to investigate differences in the metabolic composition of organic extracts of Phaeoacremonium species culture filtrates.

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Section: Introductionmentioning

confidence: 81%

Untargeted and Targeted LC-MS/MS Based Metabolomics Study on In Vitro Culture of Phaeoacremonium Species

Reveglia

Raimondo

Cimmino³

et al. 2022

JoF

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“…Over the last two decades, a general rise of GTD diseases has been confirmed, not only of esca and Eutypa dieback, but also others such as Botryosphaeria dieback, Petri disease, and black-foot disease [ 9 , 10 ]. These last three seem to affect young vineyards, while esca and Eutypa dieback do so with older vineyards [ 11 ]. Petri pathogens are associated with esca in mature vineyards as well, and this co-infection is known as the esca complex [ 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…These last three seem to affect young vineyards, while esca and Eutypa dieback do so with older vineyards [ 11 ]. Petri pathogens are associated with esca in mature vineyards as well, and this co-infection is known as the esca complex [ 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Physiological and Molecular Responses of Vitis vinifera cv. Tempranillo Affected by Esca Disease

et al. 2022

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Esca is a multi-fungal disease affecting grapevines. The objective of the study was to evaluate the physiological and molecular response of the grapevine cv. Tempranillo to esca disease, carried out in a vineyard under Mediterranean climatic conditions in western Spain. The photosynthetic pigments in the leaves decreased, with a strong decrease in the photosynthetic efficiency. The proline content increased significantly in the early stages of affected leaves, being possibly involved in the maintenance of lipid peroxidation levels in leaves, which do not increase. The phenol, flavonoid, and phenylpropanoid content decreased in esca-affected leaves, as does the total antioxidant capacity (FRAP), while the polyphenol oxidase (PPO) activity suffers a strong increase with the development of the disease. In affected grapes, the lipid peroxidation and the total phenol content decrease, but not the anthocyanin content. The ascorbate pool decreases with the disease and with time. On the other hand, pool GSH + GSSG is lower in affected leaves, but increases with time. These alterations show a clear change in the redox homeostasis. The expression of genes phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), superoxide dismutase (SOD), and chalcone synthase (ChaS1 and ChaS3) become considerably higher in response to esca, being even higher when the infection time increases. The alteration of AsA and GSH levels, phenolic compounds, PPO activity, proline content, and FRAP, together with the increase of the PAL, PPO, SOD,ChaS1, and ChaS3 gene expression, are clearly implicated in the esca response in plants. The expression of these genes, similar to the PPO activity, can be used as markers of state in the development of the disease.

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“…Diseases caused by fungi are one of the limiting factors when it comes to vine cultivation, preventing the expansion of production and causing considerable losses from the replacement of affected vineyards (Hofstetter et al 2012). Grapevine trunk diseases (GTD) have been considered one of the most serious problems affecting vineyards, both young and old (Agust ı-Brisach et al 2019). The term GTD has been used to include symptoms observed in the foliage and vascular tissue of vines, caused by a group of fungi that mainly infect plants through pruning wounds, and then colonize their vascular tissues (Mugnai, 2011).…”

Section: Introductionmentioning

confidence: 99%

The efficacy of 8‐hydroxyquinoline derivatives in controlling the fungusIlyonectria liriodendri, the causative agent of black foot disease in grapevines

Souza

Chaves

Joaquim

et al. 2021

J of Applied Microbiology

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Aim: The purpose of this study was to evaluate the in vitro and in vivo efficiency of derivatives of 8-Hydroxyquinoline (8HQ) in controlling the fungus Ilyonectria liriodendri. Methods and Results: The in vitro tests consisted of assessing its susceptibility to the minimal inhibitory concentration (MIC) and the inhibition of mycelial growth. While the in vivo tests consisted of applying and assessing the most effective products for the protection of wounds, in both preventive + curative and curative forms. The MIC values for PH 151 (6Á25 µg ml À1 ) showed better results when compared to the fungicides tebuconazole (>50 µg ml À1 ) and mancozeb (12Á5 µg ml À1 for strain 176 and 25 µg ml À1 for strain 1117). PH 151 significantly inhibited mycelial growth, while mancozeb did not differ from the control. In in vivo tests, PH 151 again demonstrated excellent results in vitro, especially when applied preventively. Conclusions: The derivative of 8HQ PH 151 was effective in controlling the fungus I. liriodendri in vitro and proved to be a promising option for protecting wounds. Significance and Impact of the Study: This study points to the prospect of an effective and safe preventive antifungal product, which would enable the use of pesticides in vine culture to be reduced.

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Occurrence of grapevine trunk diseases affecting the native cultivar Pedro Ximénez in southern Spain

Cited by 16 publications

References 47 publications

Untargeted and Targeted LC-MS/MS Based Metabolomics Study on In Vitro Culture of Phaeoacremonium Species

Untargeted and Targeted LC-MS/MS Based Metabolomics Study on In Vitro Culture of Phaeoacremonium Species

Physiological and Molecular Responses of Vitis vinifera cv. Tempranillo Affected by Esca Disease

The efficacy of 8‐hydroxyquinoline derivatives in controlling the fungusIlyonectria liriodendri, the causative agent of black foot disease in grapevines

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