“…By using a standard method (Comar and Zscheile, 1942) for determining Chi in plant tissue, a method which gives an acceptable value for spinach of about 70% Chi a (Comar and Zscheile, 1942; Leowenschuss and Wakelyn, 1972a), green bract was found to be 65% Chi a and green leaf to be 71% Chi a. Methanol, therefore, was used as the extracting solvent. By tic, the methanol extract contained three components |as yet unidentified; none of which were gossypol [gossypol was identified separately, under different conditions, as a component of dried bract (Loewenschuss and Wakelyn, 1972b)]} in addition to Chi a and b; after partitioning with CCI4, no Chi, only these three compounds, remain in the methanol. Traces of one of the additional components were found by tic along with Chi a and Chi b in the CCI4 layer.…”