Occurrence of Gluconacetobacter diazotrophicus in tropical and subtropical plants of Western Ghats, India

Madhaiyan, Munusamy; Saravanan, Venkatakrishnan Sivaraj; Jovi, D.Bhakiya Silba Sandal; Choi, Yongrok; Thenmozhi, R.; Hari, K.; Sa, Tongmin

doi:10.1016/j.micres.2004.04.001

Cited by 65 publications

(43 citation statements)

References 23 publications

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“…The samples were macerated in 1% sucrose solution using a low-speed blender for 30 s. The homogeneous mixture obtained was serially diluted from 10 -1 to 10 -3 ; then, 100 μL aliquots were plated in triplicate in 6-mL vials containing 3 mL of semisolid LGI-P medium (Cavalcante and Döbereiner, 1988;Madhaiyan et al, 2004).…”

Section: Isolation Of Gluconacetobacter Sppmentioning

confidence: 99%

“…For this reaction, the universal primers RB and RM (Madhaiyan et al, 2004) were used; the expected fragment was approximately 800 bp. The final volume of the PCR reaction was adjusted to 25 µL (1X PCR buffer, 0.2 mM dNTPs, 1 mM MgCl2, 0.5 μM primers, 1 U of polymerase taq DNA recombinant polymerase by Invitrogen and 100 ng of bacterial DNA).…”

Section: Pcr Identification Of Isolatesmentioning

confidence: 99%

“…This bacterium has been isolated from sugarcane cultivars in different countries (Bellone et al, 1997;Fuentes et al, 1993;Li and Macrae, 1991). Likewise, it has been recovered from other crops like coffee (Jiménez et al, 1997), pineapple (Tapia et al, 2000), carrot (Madhaiyan et al, 2004), and rice (Muthukumarasamy et al, 2005). G. diazotrophicus is a subject of great interest among nitrogen-fixing bacteria, as it fixes nitrogen under aerobic growth conditions (Eskin et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of plant-growth promoting properties of Gluconacetobacter diazotrophicus and Gluconacetobacter sacchari isolated from sugarcane and tomato in West Central region of Colombia

Franco¹,

Marulanda²,

Galeano³

et al. 2017

Afr. J. Biotechnol.

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The genus Gluconacetobacter comprises different species with agricultural and industrial importance. This study aims at determining the presence of Gluconacetobacter diazotrophicus and Gluconacetobacter sacchari in sugarcane and tomato crops considering their potential biotechnological applications. Bacteria were isolated from roots, stems and leaf tissues and identified using phenotypic and biochemical evaluations, and molecular and phylogenetic analyses. Isolates were characterized by the production of indolic compounds, nitrogenase activity and phosphate solubilization. For this, G. diazotrophicus ATCC 49037 was used as the reference strain. The results showed that all isolates of native G. diazotrophicus exhibited equal or better phosphate solubilization index (SI) for CaCO 3 and AlPO 4 than reference strain. For G. sacchari, GIBI031 isolate displayed better SI for both phosphate sources and GIBI014 isolate had better SI only for AlPO 4 . G. diazotrophicus GIBI029 had a greater production of indolic compounds than ATCC 49037 strain in the presence of tryptophan. All isolates except G sacchari GIBI031, showed better nitrogenase activity than the control. These results constitute the first report confirming the presence of G. diazotrophicus and G. sacchari associated with sugarcane in Colombia. In addition, this is the first report on the presence of G. sacchari in tomato under natural conditions. Finally, one G. sacchari isolate presented nitrogenase activity despite the fact that this is a differential characteristic between G. diazotrophicus and G. sacchari. These findings have ecological significance and will advance research towards the evaluation of plant-soil interactions involving these bacteria in crops other than sugarcane. The isolates found are potential candidates for the development of novel biotechnological processes for production of new alternative biofertilizers considering the significant plant-growth promotion properties determined in this work.

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Section: Isolation Of Gluconacetobacter Sppmentioning

confidence: 99%

Section: Pcr Identification Of Isolatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of plant-growth promoting properties of Gluconacetobacter diazotrophicus and Gluconacetobacter sacchari isolated from sugarcane and tomato in West Central region of Colombia

Franco¹,

Marulanda²,

Galeano³

et al. 2017

Afr. J. Biotechnol.

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“…Among the different microorganisms used in sweet corn cultivation, the nitrogen fixing microorganisms like Acetobacter, phosphorous solubilizing bacteria and potassium solubilizing bacteria are used extensively in single, double and triple inoculant formulation however the native efficient isolates are not concentrated much in sweet corn production. Madhaiyan et al, (2004) Conceptual design is important in developing new technologies and also utilization of different beneficial plant growth promoting rhizomicroorganisms for sustainable vegetable production. The basis of conceptual design is simply to first convince a model and then to devise a strategy and method for achieving the reality.…”

mentioning

confidence: 99%

Development and Evaluation of Native Plant Growth Promoting Rhizomicrobial Consortia on Growth Parameters of Sweet Corn

Nandish¹,

Suchitha²,

Madhuri³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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“…Several diazotrophs have been isolated and characterized as nitrogen-fixing endophytes in plants, including Gluconacetobacter diazotrophicus 3,22) , Herbaspirillum spp. 9,33,38,48) , and Azoarcus sp.…”

mentioning

confidence: 99%

Broad Distribution and Phylogeny of Anaerobic Endophytes of Cluster XIVa Clostridia in Plant Species Including Crops

et al. 2008

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Endophytic clostridia present on various plants as obligate anaerobes were surveyed by terminal restriction fragment length polymorphism (TRFLP) analysis specific to the clostridial 16S rRNA gene. Endophytic clostridia were detected in 10 plant types: sugarcane, cultivated rice, corn, tobacco, soybean, bermuda grass, tall fescue, and three mangrove species. Phylogenetically, cluster XIVa clostridia were detected more frequently than cluster I clostridia in aerial parts. Isolation of clostridia from surface-sterilized sugarcane stem validated the TRFLP results. Plant-derived clostridia occupied two unique phylogenetic positions (groups I and II) within cluster XIVa. Most of cluster XIVa clostridia from other sources (e.g., human, animal, and insect intestines) were located outside these groups. Thus two unique groups of cluster XIVa clostridia are widely distributed in plants, including crops. In field-grown soybeans, TRFLP analysis revealed clostridia only in a non-nodulating mutant. Ribosomal intergenic spacer analysis (RISA) showed that the bacterial community in soybean shoot depended partly on the soybean nodulation genotype.Key words: Clostridium, cluster XIVa, endophyte, sugarcane, soybean Endophytes are microorganisms that can colonize living plant tissues without causing any apparent damage to the host 3,23,24,35) . Several diazotrophs have been isolated and characterized as nitrogen-fixing endophytes in plants, including Gluconacetobacter diazotrophicus 3,22) , Herbaspirillum spp. 9,33,38,48) , and Azoarcus sp.19,31) . Novel anaerobic endophytic clostridia and their anaerobic nitrogen-fixing consortium (ANFICO) were recently isolated and characterized in grasses during attempt to overcome a problem with culturing nitrogen-fixing microbes 26) . ANFICO consists of nitrogenfixing clostridia and diverse non-diazotrophic bacteria and expresses nitrogen-fixing activity associated with low oxygen tension and interspecies inducers 26) . Phylogenetic analysis indicated that the endophytic clostridia fell exclusively into clusters XIVa and I, as defined by Collins et al. 8) , and were further subdivided into five groups 26) . Miyamoto et al.27) developed a terminal restriction fragment length polymorphism (TRFLP) detection system specific for these clusters and groups of plant clostridia, and demonstrated that the group II clostridia in cluster XIVa 8)dominated the populations of diazotrophs and clostridia in a gramineous grass, Miscanthus sinensis. Inoculation examinations indicate that endophytic clostridia colonize plant tissues, alleviating the damage caused by salt stress 47) and potentially fixing nitrogen in the plant 34) . Thus, more attention should be paid to the endophytic clostridia in terms of their distribution, phylogeny, and function.Recently, cluster XIVa clostridia have been detected and isolated from human faeces 12,18,28) , animal intestines 2,20,21,25,44) , termite guts 40,41) , soil 7,17,45) , and plants 26,27) using culture-independent and culture-dependent methods. However, the relations...

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Occurrence of Gluconacetobacter diazotrophicus in tropical and subtropical plants of Western Ghats, India

Cited by 65 publications

References 23 publications

Evaluation of plant-growth promoting properties of Gluconacetobacter diazotrophicus and Gluconacetobacter sacchari isolated from sugarcane and tomato in West Central region of Colombia

Evaluation of plant-growth promoting properties of Gluconacetobacter diazotrophicus and Gluconacetobacter sacchari isolated from sugarcane and tomato in West Central region of Colombia

Development and Evaluation of Native Plant Growth Promoting Rhizomicrobial Consortia on Growth Parameters of Sweet Corn

Broad Distribution and Phylogeny of Anaerobic Endophytes of Cluster XIVa Clostridia in Plant Species Including Crops

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