Occurrence of Giardia and Cryptosporidium in water supplies of Russia and Bulgaria

Karanis, Panagiotis; Sotiriadou, Isaia; Kartashev, Vladimir; Kourenti, C.; Tsvetkova, Nina; Stojanova, K.

doi:10.1016/j.envres.2006.05.005

Cited by 95 publications

(46 citation statements)

References 29 publications

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“…Use of PCR allowed investigators to detect and genotype 8.4% of Giardia in Portugal [59] and 33.8% in Spain [51] . Using IFA, detection rates of 9.6% and 45.6% were reported in Russia [61] and Spain [51] , respectively. In the Russian study, the investigators attributed the lower detection rate to the low sensitivity of the immunofluorescent staining methods used.…”

Section: Discussionmentioning

confidence: 99%

Detection rates of waterborne protozoa in water sources from Fayoum Governorate

Sakran

El-Shahawy

Shalaby

et al. 2017

Parasitologists United Journal

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Background: Waterborne protozoal infections are common health problems in many parts of the world especially in developing countries. Water is a major vehicle for transmission of protozoa such as G. lamblia, Cryptosporidium spp. as well as pathogenic and opportunistic free living amoeba (FLA). Objective: This study aims to detect the presence of protozoal agents in tap water and storage water tanks at Fayoum Governorate, Egypt. Material and Methods: A total of ninety five water samples were collected from different water sources, taps (65) and tanks (30), from 6 Fayoum districts. The samples were processed to detect the presence of G. lamblia cysts by Lugol's iodine stain, Cryptosporidium oocysts by modified Ziehl-Neelsen stain and FLA by cultivation. After cultivation, Acanthamoeba spp. were identified according to their morphological features and flagellation test was performed to detect amoeboflagellates. Results: All water samples collected from tanks (100%) were contaminated by protozoa of medical importance, while only 6 (9.2%) of the tap water samples were pathogen free. The majority of water samples were contaminated with mixed protozoal infections. The overall detection rates of contaminants in water sources were 86.3%, 52.6%, 13.7% by FLA, Cryptosporidium spp. and G. lamblia, respectively. Conclusions: The recorded detection rates of waterborne protozoa present a hazard to the community resulting in silent morbidities and mortalities. It is strongly recommended to adopt proper water safety measures.

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Section: Discussionmentioning

confidence: 99%

Detection rates of waterborne protozoa in water sources from Fayoum Governorate

Sakran

El-Shahawy

Shalaby

et al. 2017

Parasitologists United Journal

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“…The collected water from each sample was decanted to dark glass bottle to perform Al 2 (SO 4 ) 3 flocculation. All collected water samples around Ordu at the Middle Black Sea area from different sources were concentrated by Al 2 (SO 4 ) 3 -flocculation and concentrated as described by Karanis and Kimura (2002) and as it has been applied by Kourenti et al (2003) and Karanis et al (2006).…”

Section: Water Sampling Sitesmentioning

confidence: 99%

“…IFT was subsequently applied in all collected water samples and it was performed in Eppendorf tubes according to the modified protocol of Karanis et al (2006). Briefly, defined 50 ml pellet aliquots out of 1 ml pellet, corresponding to 5% of the whole water sample, were once washed with distilled water and concentrated by centrifugation at 8000 rev/minute for 5 minutes.…”

Section: Microscopic Detection and Identification Of Cryptosporidium mentioning

confidence: 99%

Investigations and comparative detection ofCryptosporidiumspecies by microscopy, nested PCR and LAMP in water supplies of Ordu, Middle Black Sea, Turkey

Kolören

Sotiriadou

Karanis

2011

Annals of Tropical Medicine & Parasitology

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A total of 70 water samples, including tap, river, fountain and well water were collected in the Ordu province, Middle Black Sea, Turkey and investigated for the detection of Cryptosporidium oocysts. The samples were directly screened microscopically for Cryptosporidium oocysts' detection by immunofluorescence test and subsequently DNA was extracted for the molecular detection by loop-mediated isothermal amplification (LAMP) and nested polymerase chain reaction (PCR). Eighteen out of the 70 (25.7%) water samples were found positive for Cryptosporidium spp. by immunofluorescence test and 19 (27.1%) were found positive by LAMP. Nested PCR products were not generated in any of the investigated water samples. A total of 16 randomly selected pellets were spiked with 10 Cryptosporidium oocysts to test the efficiency of the applied method. All the samples were found positive by LAMP for the presence of Cryptosporidium DNA, while the nested PCR assay was positive in only seven (43.75%) out of the 16 examined spiked samples. This is the first report on the occurrence of Cryptosporidium species in environmental and drinking water supplies in the Black Sea area.

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“…Five litters of water samples from investigated area were collected and they were flocculated with treatment in Aluminum Sulfate [Al2(SO4)3] and concentrated by sucrose gradient centrifugation for water pellets as described by Karanis and Kimura [6] and Kourenti et al [7] and Karanis et al [5] Koloren et al [8] …”

Section: Aluminum Sulfate Flocculation and Sucrose Gradient Centrifugmentioning

confidence: 99%

LAMP DETECTION of Cryptosporidium SPECIES in SURFACE WATER SAMPLES COLLECTED from RIVER YESILIRMAK and STREAM TERSAKAN (Samsun-Amasya)

Kolören¹

2017

ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology

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Occurrence of Giardia and Cryptosporidium in water supplies of Russia and Bulgaria

Cited by 95 publications

References 29 publications

Detection rates of waterborne protozoa in water sources from Fayoum Governorate

Detection rates of waterborne protozoa in water sources from Fayoum Governorate

Investigations and comparative detection ofCryptosporidiumspecies by microscopy, nested PCR and LAMP in water supplies of Ordu, Middle Black Sea, Turkey

LAMP DETECTION of Cryptosporidium SPECIES in SURFACE WATER SAMPLES COLLECTED from RIVER YESILIRMAK and STREAM TERSAKAN (Samsun-Amasya)

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