Occurrence of four species of algae in the marine water of Hong Kong

Chai, Y.M.; Deng, Wen-Jing; Xing, Qin; Xu, Xiangrong

doi:10.1016/j.marpolbul.2016.12.043

Cited by 8 publications

(5 citation statements)

References 20 publications

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“…Several studies on the DNA analyses of microalgae have shown that molecular techniques have promising applications for bioassays in aquatic ecosystems, especially for detecting HAB species. Several molecular methods have been developed for the rapid and accurate detection of harmful microalgae, including cell-targeting techniques, such as fluorescence in situ hybridization (FISH) (Hosoi-Tanabe and Sako 2005;Hattenrath-Lehmann et al 2016), and cell-free techniques, such as sandwich hybridization assay (Zhen et al 2009, Zhang et al 2014, qualitative PCR (qPCR) (Chai et al 2017;Engesmo et al 2018), lab on a chip (Loukas et al 2017), multiplex PCR (mPCR) (Sun et al 2019), and digital PCR (Lee et al 2020). However, the aforementioned molecular techniques are time-consuming and rely on special instruments, high-cost testing reagents, and professional operators.…”

Section: Introductionmentioning

confidence: 99%

Recombinase-aided amplification combined with lateral flow dipstick for the rapid detection of Amphidinium carterae

Zhang

Liu

et al. 2021

J Appl Phycol

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In the context of global climate change, the frequency and duration of harmful algal blooms (HABs) due to eutrophic coastal waters have increased. HABs can cause marine ecological imbalance, massive socio-economic damage, and potential public health problems. Therefore, accurate and specific techniques that can rapidly detect harmful algae are critical to expedite early monitoring and prediction of HABs. Although some methods based on amplification have previously been described, limitations associated with limited popularity and high costs still exist. In this study, the partial sequence of nuclear ribosomal DNA from Amphinidium carterae was selected as the target region. The biotin-labeled recombinase-aided amplification (RAA) products and the 6-carboxyfluorescein-labeled probe were specifically conjugated, which formed double-labeled RAA products analyzed by lateral flow dipstick (LFD). A rapid RAA-LFD assay aimed at visually detecting A. carterae was established and optimized. The specificity and sensitivity of RAA-LFD were determined. The nucleic acid test with other control algal species yielded negative results, which indicated that RAA-LFD assay can specifically detect A. carterae. The detection limit of RAA-LFD assay for A. carterae was 8.49 ng μL −1 genomic DNA and 1 cell mL −1 . The whole detection process of RAA-LFD could be completed within 50 min. In conclusion, the proposed RAA-LFD assay may be promising for the efficient, specific, and rapid detection of A. carterae in monitoring efforts.

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Section: Introductionmentioning

confidence: 99%

Recombinase-aided amplification combined with lateral flow dipstick for the rapid detection of Amphidinium carterae

Zhang

Liu

et al. 2021

J Appl Phycol

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“…Harmful algal blooms (HABs), also referred to as red tides, arise from the rapid multiplication of microalgae that are toxic or harmful to marine animals such as fish, shellfish, marine mammals, and seabirds, and their toxins may cause diseases in humans who consume contaminated water or food [1]. HABs show adverse impacts in many aspects, such as marine biodiversity, aquafarming, and marine recreational activities [2]. Some HAB species produce toxins that damage fish gills or mucus, both of which affect breathing, as problems in mucus could lead to clogged gills [3,4].…”

Section: Introductionmentioning

confidence: 99%

“…Protein spots starting with B showed at least two-fold changes at both LT 50 and LT 90 but not LT 25 , while those starting with C showed at least two-fold changes at LT 90 only 2. Scores greater than 58 are significant (p < 0.05) 3.…”

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confidence: 97%

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Proteome Analysis of Whole-Body Responses in Medaka Experimentally Exposed to Fish-Killing Dinoflagellate Karenia mikimotoi

Kwok

Lai

Lam

et al. 2021

IJMS

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Karenia mikimotoi is a well-known harmful algal bloom species. Blooms of this dinoflagellate have become a serious threat to marine life, including fish, shellfish, and zooplanktons and are usually associated with massive fish death. Despite the discovery of several toxins such as gymnocins and gymnodimines in K. mikimotoi, the mechanisms underlying the ichthyotoxicity of this species remain unclear, and molecular studies on this topic have never been reported. The present study investigates the fish-killing mechanisms of K. mikimotoi through comparative proteomic analysis. Marine medaka, a model fish organism, was exposed to K. mikimotoi for a three-part time period (LT25, LT50 and LT90). Proteins extracted from the whole fish were separated by using two-dimensional gel electrophoresis, and differentially expressed proteins were identified with reference to an untreated control. The change in fish proteomes over the time-course of exposure were analyzed. A total of 35 differential protein spots covering 19 different proteins were identified, of which most began to show significant change in expression levels at the earliest stage of intoxication. Among the 19 identified proteins, some are closely related to the oxidative stress responses, energy metabolism, and muscle contraction. We propose that oxidative stress-mediated muscle damage might explain the symptoms developed during the ichthyotoxicity test, such as gasping for breath, loss of balance, and body twitching. Our findings lay the foundations for more in-depth studies of the mechanisms of K. mikimotoi’s ichthyotoxicity.

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“…The frequent occurrence of harmful algal blooms (HABs), which happens due to increasing water pollution and climate changes, is harmful to the environment, economy, and public health [1,2,3]. HABs can directly or indirectly poison aquatic animals and humans, discolor coastal waters and harm water quality, form fetid foam with unpleasant smell, damage light-dependent aquatic ecosystems, and hinder the tourism industry [4].…”

Section: Introductionmentioning

confidence: 99%

The Antialgal Mechanism of Luteolin-7-O-Glucuronide on Phaeocystis globosa by Metabolomics Analysis

Zhu

Yang

Duan

et al. 2019

IJERPH

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Antialgal compounds from plants have been identified as promising candidates for controlling harmful algal blooms (HABs). In our previous study, luteolin-7-O-glucuronide was used as a promising algistatic agent to control Phaeocystis globosa (P. globose) blooms; however, its antialgal mechanism on P. globosa have not yet been elaborated in detail. In this study, a liquid chromatography linked to tandem mass spectrometry (LC-MS/MS)-based untargeted metabolomic approach was used to investigate changes in intracellular and extracellular metabolites of P. globosa after exposure to luteolin-7-O-glucuronide. Significant differences in intracellular metabolites profiles were observed between treated and untreated groups; nevertheless, metabolic statuses for extracellular metabolites were similar among these two groups. For intracellular metabolites, 20 identified metabolites showed significant difference. The contents of luteolin, gallic acid, betaine and three fatty acids were increased, while the contents of α-Ketoglutarate and acetyl-CoA involved in tricarboxylic acid cycle, glutamate, and 11 organic acids were decreased. Changes in those metabolites may be induced by the antialgal compound in response to stress. The results revealed that luteolin played a vital role in the antialgal mechanism of luteolin-7-O-glucuronide on P. globosa, because luteolin increased the most in the treatment groups and had strong antialgal activity on P. globosa. α-Ketoglutarate and acetyl-CoA were the most inhibited metabolites, indicating that the antialgal compound inhibited the growth through disturbed the tricarboxylic acid (TCA) cycle of algal cells. To summarize, our data provides insights into the antialgal mechanism of luteolin-7-O-glucuronide on P. globosa, which can be used to further control P. globosa blooms.

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Occurrence of four species of algae in the marine water of Hong Kong

Cited by 8 publications

References 20 publications

Recombinase-aided amplification combined with lateral flow dipstick for the rapid detection of Amphidinium carterae

Recombinase-aided amplification combined with lateral flow dipstick for the rapid detection of Amphidinium carterae

Proteome Analysis of Whole-Body Responses in Medaka Experimentally Exposed to Fish-Killing Dinoflagellate Karenia mikimotoi

The Antialgal Mechanism of Luteolin-7-O-Glucuronide on Phaeocystis globosa by Metabolomics Analysis

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