Occurrence of extended-spectrum cephalosporinase producing Escherichia coli in kuroiler birds

Abstract

the present study was undertaken to detect the incidence of extended-spectrum-cephalosporinase gene possessing-Escherichia coli, its co-resistance pattern against other antimicrobials, and the clonal relationship of the isolates in healthy kuroiler birds. a total of 80 cloacal swabs from kuroilers were collected randomly from West Bengal, India. the use of costly antimicrobials (cephalosporins) was not practiced by farmers. Escherichia coli was isolated and identified by standard biochemical tests and 16SrRNA-PCR. All the E. coli isolates, including controls, were subjected to PCr for detection of bla CTX-M , bla TEM, bla SHV, and bla CMY-2 genes. By comparing the raPD-banding pattern, the phylogenetic relationship among the isolates was established. all the isolates were tested for phenotypical resistance against other antibiotics. In total, 60 isolates were identified as E. coli from the kuroilers studied (n = 80). among them, 12 (20%) isolates possessed one of the studied extended-spectrum cephalosporinase genes. among the studied genes, bla TEM and bla SHV were detected in 6 (10%) and 12 (20%) E. coli isolates, respectively. None of the E. coli isolates possessed bla CTX-M and bla In phylogenetic analysis, the strains isolated from same localities with similar genetic profile were grouped into the same cluster. resistance of extended-spectrum cephalosporinase gene possessing E. coli isolates was observed most frequently against ampicillin/cloxacillin, co-trimoxazole, amoxyclav, piperacillin, ceftriaxone, and tetracycline. Kuroiler birds with no cephalosporin usage profile may act as a reservoir of extended-spectrum cephalosporinase gene possessing E. coli. This is the first systematic study in kuroilers, to raise the awareness of consumers regarding the possibility of transmission of antimicrobial resistant E. coli from them.

Evaluation of the Relationship Between Virulence, Antibiotic Resistance Genes and Development of Biofilm in Escherichia Coli Isolated From Broiler Chicken

“…Among food animals that act as reservoirs of ESBLproducing E. coli, broilers were considered to be the most potent reservoir Pacholewicz et al (2015).In this study the percentage of blaTEM gene from the isolated E .coli strains was 100 % (10 out of 10 strains) which nearly go hand with the results of Maram (2014) who detected blaTEM gene in 18 out of 19 E.coli isolates with 94.73%. But these results disagreed with Ghosh et al (2017) who obtained lower percentage about 10%from isolated E. coli.…”

Extended-spectrum ß-lactamase-producing Escherichia coli among humans, chickens and poultry environments in Abuja, Nigeria

“…The mechanism of spread of antibiotic resistance from animals to humans and vice versa remains controversial. Studies have reported that E. coli which can be isolated from water, food and farm animals is associated with antimicrobial resistance [ 14 ].…”

Antimicrobial Resistance: A Menace to Food Chain

“…This investigation revealed that the studied birds harbour CTXC-M-producing Klebsiella spp. which might be transmitted to the food chain either directly or indirectly [13,14].…”