Occurrence of Estrogen Receptor α in Bovine Placentomes Throughout Mid and Late Gestation and at Parturition1

Schuler, Gerhard; Wirth, C; Teichmann, Ursula; Failing, Klaus; Leiser, Rudolf; Thole, Hubert; Hoffmann, Bernd

doi:10.1095/biolreprod66.4.976

Cited by 45 publications

(44 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Observations in placentomes from a cow after induction of parturition and in a cow at normal term indicates that prepartal up-regulation of aromatase activity (Tsumagari et al 1993) follows a pattern similar to the one identified for P450c17. The up-regulation of P450arom and oestrogen receptor b (Schuler et al 2005) concomitant with TGC differentiation points to an autocrine regulatory role of placental oestrogens during this process in addition to their previously postulated functions as local caruncular growth factor (Schuler et al 2002). Accordingly, a similar role of placental oestrogens has been suggested previously in the differentiation of syncytiotrophoblast from cytotrophoblast in the human placenta (Bukovsky et al 2003, Rama et al 2004.…”

Section: Day 90supporting

confidence: 67%

“…In cattle, our preceding studies suggest a particular role of placental progesterone and oestrone as local regulators of placental growth and differentiation (Hoffmann et al 1997, Schuler et al 1999, 2005, Hoffmann & Schuler 2002. Sites of placental steroid production are the fetal cotyledons (Hoffmann et al 1979, Conley & Ford 1987, Schuler et al 1994 which, together with the maternal caruncular tissue, form multiple discrete sites of placentation, the placentomes (Leiser & Kaufmann 1994).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Reciprocal expression of 17α-hydroxylase-C17,20-lyase and aromatase cytochrome P450 during bovine trophoblast differentiation: a two-cell system drives placental oestrogen synthesis

Schuler¹,

Özalp²,

Hoffmann³

et al. 2006

Reproduction

View full text Add to dashboard Cite

No definitive information is yet available on the steroidogenic capacity of the two morphologically distinct cell types forming the bovine trophoblast, the uninucleated trophoblast cells (UTCs) and the trophoblast giant cells (TGCs). Hence, in order to localise 17a-hydroxylase-C17,20-lyase (P450c17) on a cellular level and to monitor its expression as a function of gestational age, placentomes from pregnant (days 80 -284; n 5 19), prepartal (days 273 -282; 24 -36 h prior to the onset of labour; n 5 3) and parturient cows (n 5 5) were immunostained for P450c17 using an antiserum against the recombinant bovine enzyme. At all stages investigated, P450c17 was exclusively found in the UTCs of chorionic villi (CV), where staining was ubiquitous between days 80 and 160, but was largely restricted to primary CV and the branching sites of secondary CV between days 160 and 240. Thereafter, a distinct ubiquitous staining reoccurred in the UTCs of all CV in late pregnant, prepartal and parturient animals. Using an antiserum against human aromatase cytochrome P450 (P450arom), specific cytoplasmic staining was observed in TGCs. In placentomes from pregnant cows, staining intensity was higher in mature compared with immature TGCs and was more pronounced in the trophoblast covering big stem villi compared with the trophoblast at other sites of the villous tree. In placentomes of a parturient cow, specific staining was only found in mature TGCs that survived the normal, but substantial, prepartal decline in TGC numbers. These results clearly showed that bovine UTCs and TGCs exhibit different steroidogenic capacities, constituting a 'two-cell' organisation for oestrogen synthesis. P450c17 expression appears to be quickly down-regulated and P450arom is up-regulated when UTCs enter the TGC differentiation pathway.

show abstract

Section: Day 90supporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Reciprocal expression of 17α-hydroxylase-C17,20-lyase and aromatase cytochrome P450 during bovine trophoblast differentiation: a two-cell system drives placental oestrogen synthesis

Schuler¹,

Özalp²,

Hoffmann³

et al. 2006

Reproduction

View full text Add to dashboard Cite

show abstract

“…Western blotting This was carried out according to Schuler et al (2002) with modifications. Samples (50 µg protein in 20 µl buffer) were boiled in 3 loading buffer (10 mM Tris-HCl, pH 6·8 including 3% SDS, 5% -mercaptoethanol, 20% glycerol and 0·6% bromophenol blue for 3 min and separated on a commercially available 4-12% Bis-Tris gel (Invitrogen, Karlsruhe LMA, Germany) under reducing conditions (for 1 h at 190 V), and transferred to nitrocellulose membranes (Millipore PVDF, 0·45 µm; Millipore, Bedford, USA).…”

Section: Western Blotmentioning

confidence: 99%

Expression and localisation of oestrogen and progesterone receptors in the bovine mammary gland during development, function and involution

Schams¹,

Kohlenberg²,

Amselgruber³

et al. 2003

Journal of Endocrinology

View full text Add to dashboard Cite

It is now well established that oestrogen and progesterone are absolutely essential for mammary gland development. Lactation can be induced in non-pregnant animals by sex steroid hormone treatment. Most of the genomic actions of oestrogens are mediated by two oestrogen receptors (ER)-and ER , and for gestagens in ruminants by the progesterone receptor (PR). Our aim was the evaluation of mRNA expression and protein (localisation and Western blotting) during mammogenesis, lactogenesis, galactopoiesis (early, middle and late) and involution (8, 24, 28,(96)(97)(98)(99)(100)(101)(102)(103)(104)(105)(106)(107)(108) h and 14-28 days after the end of milking) in the bovine mammary gland (total no. 53). During these stages, the mRNA was assessed by means of real-time RT-PCR (LightCycler). The protein for ER , ER and PR was localised by immunohistochemistry and Western blotting. The mRNA expression results indicated the existence of ER , ER and PR in bovine mammary gland. Both ER and PR are expressed in fg/µg total RNA range. The highest mRNA expression was found for ER and PR in the tissue of non-pregnant heifers, followed by a significant decrease to a lower level at the time of lactogenesis with low concentrations remaining during lactation and the first 4 weeks of involution. In contrast, the expression of ER was about 1000-fold lower (ag/µg total RNA) and showed no clear difference during the stages examined, with a significant increase only 2-4 weeks after the end of milking. Immunolocalisation for ER revealed a strong positive staining in nuclei of lactocytes in non-pregnant heifers, became undetectable during pregnancy, lactogenesis and lactation, and was again detectable 14-28 days after the end of milking. In contrast, PR was localised in the nuclei of epithelial cells in the mammary tissue of non-pregnant heifers, in primigravid animals, and during late lactation and involution. During lactogenesis, peak and mid lactation, fewer nuclei of epithelial cells were positive, but increased staining of the cytoplasm of epithelial cells was obvious. ER and ER protein was found in all mammary gland stages examined by Western blotting. In contrast to mRNA expression, the protein signal for ER was weaker in the tissue of non-pregnant heifers and during involution (4 weeks). ER protein showed a stronger signal (two isoform bands) in non-pregnant heifers and 4 weeks after the end of milking. This correlated with the mRNA expression data. Three isoforms of PR (A, B and C) were found by Western blotting in the tissue of non-pregnant heifers, but only isoform B remained during the following stages (lactogenesis, galactopoiesis and involution). In conclusion, the mRNA expression and protein data for ER and PR showed clear regulatory changes, suggesting involvement of these receptors in bovine mammary gland development and involution.

show abstract

“…Although we do not have data of the expression on the protein level, these findings suggest a higher responsiveness to estrogen during early pregnancy (up to 20 days), around the time embryonic implantation and placental development begins in dogs. In many mammalian species, estrogen is synthesized by the placenta and it is autocrinally responsible for placental growth and differentiation (Schuler et al, 2002). Therefore, we can speculate that estrogen plays also a role in the development and growth of the canine placenta, in a more likely endocrine manner.…”

Section: Discussionmentioning

confidence: 97%

Correlation of Oxytocin (OTR) and Estrogen Receptor (ER) mRNA in the Canine Placenta with the Detected Circulating Levels of Oxytocin and Estrogen during Pregnancy and Parturition

Veiga¹,

Milazzotto²,

Nichi³

et al. 2016

American Journal of Animal and Veterinary Sciences

View full text Add to dashboard Cite

Placenta is considered an important endocrine organ during gestation, as well as can also serve as a target organ for sex steroids and pituitary hormones, mainly for the cascade of events that trigger parturition. In order to initiate studies on the canine placental endocrinology regarding estrogen and oxytocin, the present study verified the correlation among the Estrogen Receptor alpha (ERα) and Oxytocin Receptor (OTR) gene expression in the placenta tissue with the serum concentrations of their respective hormones (estrogen and oxytocin) during canine pregnancy and parturition. Real-time PCR was performed to quantify the levels of ERα mRNA and OTR mRNA in the placenta tissue of bitches (n=46) during Early (up to 20 days of gestation; n=11), Mid (20 to 40 days; n=12) and Late Pregnancy (40 to 60 days; n=12) and Parturition (first stage of labor; n=11). Serum samples were collected for the evaluation of estradiol and oxytocin (OT) concentrations by radioimmunoassay. Placental ERα mRNA expression showed a progressive decrease along gestation towards parturition. Placental ERα mRNA expression was significantly higher until 40 days of pregnancy in comparison to Parturition. Regarding OTR mRNA expression, no statistical differences among groups were verified. The Parturition Group presented higher estrogen concentrations in relation to Early and Mid Pregnancy Groups. Serum oxytocin concentrations were not different among pregnancy and parturition. During late pregnancy, a positive correlation was observed between placental ERα mRNA and oxytocin serum concentration. In conclusion, ERα mRNA in the canine placental tissue varies in a time-dependent manner, especially during the period of placentation and placental growth. Conversely, serum estrogen concentrations do not regulate directly the changes in OT receptors expression in the canine placenta.

show abstract

Occurrence of Estrogen Receptor α in Bovine Placentomes Throughout Mid and Late Gestation and at Parturition1

Cited by 45 publications

References 26 publications

Reciprocal expression of 17α-hydroxylase-C17,20-lyase and aromatase cytochrome P450 during bovine trophoblast differentiation: a two-cell system drives placental oestrogen synthesis

Reciprocal expression of 17α-hydroxylase-C17,20-lyase and aromatase cytochrome P450 during bovine trophoblast differentiation: a two-cell system drives placental oestrogen synthesis

Expression and localisation of oestrogen and progesterone receptors in the bovine mammary gland during development, function and involution

Correlation of Oxytocin (OTR) and Estrogen Receptor (ER) mRNA in the Canine Placenta with the Detected Circulating Levels of Oxytocin and Estrogen during Pregnancy and Parturition

Contact Info

Product

Resources

About