Occurrence of cassava brown streak disease and associated <i>Cassava brown streak virus</i> and <i>Ugandan cassava brown streak virus</i> in the Comoros Islands

Azali, Hamza A; Maillot, Véronique; Cassam, Nathalie; Chesneau, Thomas; Soulezelle, Juliette; Scussel, Sarah; Karime, A. L. Abdoul; Hostachy, Bruno; Reynaud, Bernard; Roux-Cuvelier, Michel; Robène, Isabelle; Lett, Jean‐Michel

doi:10.5197/j.2044-0588.2017.036.019

Cited by 10 publications

(6 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Adams et al [ 36 ] assay was designed at a time when there were no sequences available from Comoros, but which were later identified to contain isolates that were phylogenetically distant from other East African isolates [ 51 ]. Next-generation sequencing offers a suitable approach in addressing challenges of false negative results such as the results obtained in this study where isolates of both CBSIs (CBSV and UCBSV) were detected, supporting earlier findings of the presence of CBSV and UCBSV in Comoros [ 30 , 51 ]. The sequences of CBSV isolates from the study reported here shared the highest nucleotide identity of 98.6% with the previously identified Comorian isolate with GenBank accession number MK103392 while that of UCBSV shared the highest nucleotide identity of 93.5% with isolate MK103391, also from Comoros.…”

Section: Discussionsupporting

confidence: 89%

“…Viral disease prevalence was high (greater than 80%) both for CBSD and CMD across the Comorian Archipelago. Although there is report of the presence of CBSD causing ipomoviruses (CBSIs) in Comoros [ 30 ] as well as CMD causing begomoviruses, there is no comprehensive data on the prevalence and incidences of these viral diseases published for Comoros. Isolates of CBSV and UCBSV were reported and confirmed by Sanger sequencing using PCR products obtained with primers designed by Mbanzibwa et al [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

“…Recent reports have revealed the occurrence of CBSD in the Comoros Islands [ 30 ]. Similar to CMD, there is very little information about CBSD spread in Comoros.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and Bemisia tabaci in the Comoros Islands

Shirima

Wosula

Hamza³

et al. 2022

Viruses

View full text Add to dashboard Cite

A comprehensive assessment of cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) was carried out in Comoros where cassava yield (5.7 t/ha) is significantly below the African average (8.6 t/ha) largely due to virus diseases. Observations from 66 sites across the Comoros Islands of Mwali, Ngazidja, and Ndzwani revealed that 83.3% of cassava fields had foliar symptoms of CBSD compared with 95.5% for CMD. Molecular diagnostics confirmed the presence of both cassava brown streak ipomoviruses (CBSIs) and cassava mosaic begomoviruses (CMBs). Although real-time RT-PCR only detected the presence of one CBSI species (Cassava brown streak virus, CBSV) the second species (Ugandan cassava brown streak virus, UCBSV) was identified using next-generation high-throughput sequencing. Both PCR and HTS detected the presence of East African cassava mosaic virus (EACMV). African cassava mosaic virus was not detected in any of the samples. Four whitefly species were identified from a sample of 131 specimens: Bemisia tabaci, B. afer, Aleurodicus dispersus, and Paraleyrodes bondari. Cassava B. tabaci comprised two mitotypes: SSA1-SG2 (89%) and SSA1-SG3 (11%). KASP SNP genotyping categorized 82% of cassava B. tabaci as haplogroup SSA-ESA. This knowledge will provide an important base for developing and deploying effective management strategies for cassava viruses and their vectors.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and Bemisia tabaci in the Comoros Islands

Shirima

Wosula

Hamza³

et al. 2022

Viruses

View full text Add to dashboard Cite

show abstract

“…About the same time, similar CBSD occurrences were observed across northern Tanzania and western Kenya, and over time in multiple eastern, central and southern African countries. This new epidemic in the Great Lakes region of East and Central Africa represents a major expansion of CBSD beyond the endemic zones in the coastal lowlands of eastern Africa 1,8,11–17 . The disease currently poses a major threat to cassava production in Central and West Africa including Nigeria, the world’s largest cassava producer.…”

Section: Background and Summarymentioning

confidence: 98%

Expansion of the cassava brown streak pandemic in Uganda revealed by annual field survey data for 2004 to 2017

et al. 2019

View full text Add to dashboard Cite

Cassava brown streak disease (CBSD) is currently the most devastating cassava disease in eastern, central and southern Africa affecting a staple crop for over 700 million people on the continent. A major outbreak of CBSD in 2004 near Kampala rapidly spread across Uganda. In the following years, similar CBSD outbreaks were noted in countries across eastern and central Africa, and now the disease poses a threat to West Africa including Nigeria - the biggest cassava producer in the world. A comprehensive dataset with 7,627 locations, annually and consistently sampled between 2004 and 2017 was collated from historic paper and electronic records stored in Uganda. The survey comprises multiple variables including data for incidence and symptom severity of CBSD and abundance of the whitefly vector (Bemisia tabaci). This dataset provides a unique basis to characterize the epidemiology and dynamics of CBSD spread in order to inform disease surveillance and management. We also describe methods used to integrate and verify extensive field records for surveys typical of emerging epidemics in subsistence crops.

show abstract

“…Later reports highlighted further westwards spread into parts of Central Africa ( Bigirimana et al 2011 ; Mulimbi et al 2012 ; Mulenga et al 2018 ), associated primarily with UCBSV. Further CBSD spread to the east has been reported in the Comoros Islands highlighting the spread of both CBSV and UCBSV ( Azali et al 2017 ). As opposed to the earlier spread of only UCBSV in Central Africa, more recently, mixed infections of CBSV and UCBSV have been reported in north-eastern Democratic Republic of Congo (DRC), albeit at low incidence ( Casinga et al 2019 ).…”

Section: Introductionmentioning

confidence: 97%

Genotype by environment cultivar evaluation for cassava brown streak disease resistance in Tanzania

et al. 2020

View full text Add to dashboard Cite

Highlights 17 cultivars from 5 countries evaluated for CBSD resistance at 9 sites in Tanzania. Site differences in disease pressure and vector abundance drove CBSD spread. Seven cultivars were identified with strong resistance to CBSD. CBSD-susceptible cultivars gave high yields at low disease pressure sites. CBSD control should combine host plant resistance with phytosanitary measures.

show abstract

Occurrence of cassava brown streak disease and associated Cassava brown streak virus and Ugandan cassava brown streak virus in the Comoros Islands

Cited by 10 publications

References 5 publications

Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and Bemisia tabaci in the Comoros Islands

Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and Bemisia tabaci in the Comoros Islands

Expansion of the cassava brown streak pandemic in Uganda revealed by annual field survey data for 2004 to 2017

Genotype by environment cultivar evaluation for cassava brown streak disease resistance in Tanzania

Contact Info

Product

Resources

About