Occurrence of blaNDM-7 and association with blaKPC-2, blaCTX-M15, aac, aph, mph(A), catB3 and virulence genes in a clinical isolate of Klebsiella pneumoniae with different plasmids in Brazil

Alves, Weverton de Oliveira; Scavuzzi, Alexsandra Maria Lima; Beltrão, Elizabeth Maria Bispo; Oliveira, Érica Maria de; Vasconcelos, Crhisllane Rafaele dos Santos; Rezende, Antônio Mauro; Lópes, Ana Catarina de Souza

doi:10.1007/s00203-022-03051-0

Arch Microbiol

2022

DOI: 10.1007/s00203-022-03051-0

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Occurrence of blaNDM-7 and association with blaKPC-2, blaCTX-M15, aac, aph, mph(A), catB3 and virulence genes in a clinical isolate of Klebsiella pneumoniae with different plasmids in Brazil

Weverton de Oliveira Alves

Alexsandra Maria Lima Scavuzzi

Elizabeth Maria Bispo Beltrão

et al.

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Occurrence of high-risk clones of Klebsiella pneumoniae ST11, ST340, and ST855 carrying the blaKPC-2, blaNDM-1, blaNDM-5, and blaNDM-7 genes from colonized and infected patients in Brazil

de Oliveira,

Beltrão,

Pimentel

et al. 2023

Journal of Applied Microbiology

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Aims Determine which ST clones were carrying the blaKPC, blaNDM, blaVIM, blaIMP and blaGES genes and their variants in clinical isolates of multidrug resistant K. pneumoniae. Methods and Results Ten K. pneumoniae isolates were obtained from the colonized and infected patients in a public hospital in the city of Recife-PE, in northeastern Brazil, and were further analyzed. The detection of carbapenem resistance genes and the 7 housekeeping genes (for MLST detection) were done with PCR and sequencing. The blaKPC and blaNDM genes were detected concomitantly in all isolates, with variants being detected blaNDM-1, blaNDM-5, blaNDM-7 and blaKPC-2. The blaKPC-2 and blaNDM-1 combination being the most frequent. Molecular typing by MLST detected three types of high-risk ST clones, associated with the clonal complex 258, ST11/CC258 in eight isolates, and ST855/CC258 and ST340/CC258 in the other two isolates. Conclusions These findings are worrying, as they have a negative impact on the scenario of antimicrobial resistance, and show the high genetic variability of K. pneumoniae and its ability to mutate resistance genes and risk of dissemination via different ST clones.

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Occurrence of high-risk clones of Klebsiella pneumoniae ST11, ST340, and ST855 carrying the blaKPC-2, blaNDM-1, blaNDM-5, and blaNDM-7 genes from colonized and infected patients in Brazil

de Oliveira,

Beltrão,

Pimentel

et al. 2023

Journal of Applied Microbiology

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Virulent Klebsiella pneumoniae ST11 clone carrying blaKPC and blaNDM from patients with and without COVID-19 in Brazil

Pimentel,

Beltrão,

de Oliveira

et al. 2024

Journal of Applied Microbiology

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Aims Investigated and compared the occurrence of virulence genes fimH, mrkD, irp2, entB, cps, rmpA and wabG, resistance genes blaKPC and blaNDM, and the genetic variability and clonal relationship of 29 Klebsiella pneumoniae clinical isolates of patients with and without COVID-19, from a hospital in Brazil. Methods and Results All isolates were resistant to beta-lactams. The genes were investigated by PCR, and for molecular typing, ERIC-PCR and MLST were used. The detection of blaNDM was greater (n = 23), when compared to that of blaKPC (n = 14). The virulence genes that most occurred were fimH, entB, cps and wabG, which are responsible for adhesins, siderophore enterobactin, capsule and lipopolysaccharides, respectively. Among the isolates, 21 distinct genetic profiles were found by ERIC-PCR, with multiclonal dissemination. Four isolates belonged to the ST11 clone. Conclusions The occurrence of the ST11 is worrying as it is a high-risk clone involved in the dissemination of virulent strains throughout the world.

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Occurrence of blaNDM-7 and association with blaKPC-2, blaCTX-M15, aac, aph, mph(A), catB3 and virulence genes in a clinical isolate of Klebsiella pneumoniae with different plasmids in Brazil

Cited by 2 publications

References 32 publications

Occurrence of high-risk clones of Klebsiella pneumoniae ST11, ST340, and ST855 carrying the blaKPC-2, blaNDM-1, blaNDM-5, and blaNDM-7 genes from colonized and infected patients in Brazil

Occurrence of high-risk clones of Klebsiella pneumoniae ST11, ST340, and ST855 carrying the blaKPC-2, blaNDM-1, blaNDM-5, and blaNDM-7 genes from colonized and infected patients in Brazil

Virulent Klebsiella pneumoniae ST11 clone carrying blaKPC and blaNDM from patients with and without COVID-19 in Brazil

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