Occurrence of Bis(β-Amino-β-Carboxy-ethyl) Trisulphide in the Hydrolysates of Wool and other Proteins

The occurrence of bis-(2-amino-2-carboxyethyl) trisulphide in hydrolysates of wool and other proteins

“…It appeared as a double peak when chromatographed in analytical amounts under the conditions given in Fig. 1 of Fletcher & Robson (1962a). Peaks 6 and 7 on this chromatogram are both S 1, and are believed to be stereoisomers.…”

“…Proteins. Insulin, bovine plasma albumin, lysozyme and Merino 64s quality wool were the samples used by Fletcher & Robson (1962a). Ribonuclease was obtained from British Drug Houses Ltd., and ,-lactoglobulin from L. Light and Co. Ltd.…”

“…Determinations. Thiol and disulphide were determined by the phosphotungstic acid reduction method (Fletcher & Robson, 1962a). Total S was determined by the method of Jones & Letham (1956).…”

“…Reduction of phosphotungstic acid and the reaction between bis-(2-amino-2-carboxyethyl) trisuiphide and sodium bi8ulphite. (a) Under the standard conditions described by Fletcher & Robson (1962a) for the reduction of phosphotungstic acid in the presence of sodium bisulphite, 1 4mole of Cy2S5 gave an extinction of 0-75, compared with the value 0-92 for cystine.…”

“…9 of Fig. 1 of Fletcher & Robson (1962a) is chromatographed on paper, three spots are obtained, two of which were identified with Cy2S3 and Cy2S4 by chromatographing these singly and in admixture. This gives an upper limit of 0-022 % of sulphur in the form of the tetrasulphide in a 48 hr.…”