Occurrence of
 
 satA
 and
 vgb
 Genes in Streptogramin-Resistant
 Enterococcus faecium
 Isolates of Animal and Human Origins in The Netherlands

Jensen, Lars; Hammerum, Anette M.; Aarestrup, Frank Møller; Bogaard, A E van den; Stobberingh, Ellen E.

doi:10.1128/aac.42.12.3330

Cited by 68 publications

(26 citation statements)

References 13 publications

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“…Percentages of 89% for vat(E) and 10% for vat(D) have been reported in other studies for streptogramin-resistant E. faecium isolates from broilers [37]. Both streptogramin resistance genes were also found in other studies in E. faecium isolates from animals and humans [27,38,39], with the vat(E) gene in general being more frequently detected than the vat(D) gene in E. faecium strains investigated in diverse countries in Europe [39,40]. Other mechanisms of resistance could be involved in those quinupristin/dalfopristin-resistant E. faecium strains with no genes detected, as is also suggested by other authors [41].…”

Section: Resultsmentioning

confidence: 57%

Antimicrobial resistance and the mechanisms implicated in faecal enterococci from healthy humans, poultry and pets in Portugal

Poeta

Costa

Rodrigues

et al. 2006

International Journal of Antimicrobial Agents

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Section: Resultsmentioning

confidence: 57%

Antimicrobial resistance and the mechanisms implicated in faecal enterococci from healthy humans, poultry and pets in Portugal

Poeta

Costa

Rodrigues

et al. 2006

International Journal of Antimicrobial Agents

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“…msrC, which confers resistance to streptogramin B antibiotics by active transport, is commonly found in E. faecium [33]. To date, there have not been reports of combination of 11 streptogramin A resistance gene in E. faecium; however, combinations of vatD-vgbA, vatD-ermB, and vatE-ermB have been reported [20,[34][35][36].…”

Section: Mechanisms Of Resistancementioning

confidence: 95%

“…vatD was initially found in an E. faecium isolate recovered from a hospitalized patient in Europe [16]. It has since been demonstrated in isolates of both animal and human origin outside of the hospital in Europe [17][18][19][20][21][22][23]. Transfer of vatD between isogenic E. faecium strains has also been demonstrated in vitro [19] and in vivo [24].…”

Section: Mechanisms Of Resistancementioning

confidence: 98%

Quinupristin‐Dalfopristin Resistance in Gram‐Positive Bacteria: Mechanism of Resistance and Epidemiology

et al. 2004

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Antimicrobial resistance in gram-positive bacteria is a continuing problem resulting in significant morbidity, mortality, and cost. Because of this resistance, new antimicrobial agents have been needed. Quinupristin-dalfopristin is a recently approved agent for treatment of these infections. Shortly after its introduction into clinical medicine, resistance was reported. Resistance can occur by one or more of several mechanisms, including enzymatic modification, active transport of efflux mediated by an adenosine triphosphate-binding protein, and alteration of the target site. Resistance is rare in isolates of staphylococci and Enterococcus faecium from humans. Resistance is common in isolates recovered from food animals and is related to the use of virginiamicin as a feed additive. Considering the effect antimicrobial resistance has on human health, as well as its economic impact, measures to preserve the usefulness of these agents and delay the development of resistance are urgently needed.

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“…Genes encoding resistance factors to streptogramin antibiotics and their metabolites, including the quinupristindalfopristin complex, have been identified, and their mechanisms of resistance have been determined (5)(6)(7)(8)(9)(10)(11). Resistance to the group B components of streptogramin mixtures is † This work was supported by Grant AI42154.…”

mentioning

confidence: 99%

Crystal Structure of Vat(D): An Acetyltransferase That Inactivates Streptogramin Group A Antibiotics^,

Sugantino

Roderick

2002

Biochemistry

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The streptogramin class of antibiotics act to inhibit bacterial protein synthesis, and their semisynthetic derivatives, such as dalfopristin-quinupristin (Synercid), are used to treat serious or life-threatening infections due to multiply antibiotic resistant bacteria. Acquired resistance of the nosocomial pathogen Enterococcus faecium to the group A component of natural and semisynthetic streptogramin mixtures is a prerequisite for the streptogramin resistance phenotype and is mediated by a streptogramin acetyltransferase. The crystal structure of Vat(D), a streptogramin acetyltransferase from a human urinary isolate of E. faecium, has been determined as an apoenzyme and in complex with either acetyl-CoA or virginiamycin M1 and CoA. These structures illustrate the location and arrangement of residues at the active site, and point to His 82 as a residue that may function as a general base. The structural similarity of Vat(D) to the xenobiotic acetyltransferase from Pseudomonas aeruginosa indicates similarities in the catalytic mechanism for these enzymes as well as several shared and distinctive antibiotic binding interactions between these enzymes and their respective substrates. These results reveal the molecular basis for a reaction by which Gram-positive cocci acquire resistance to a last resort antibiotic.

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Occurrence of satA and vgb Genes in Streptogramin-Resistant Enterococcus faecium Isolates of Animal and Human Origins in The Netherlands

Cited by 68 publications

References 13 publications

Antimicrobial resistance and the mechanisms implicated in faecal enterococci from healthy humans, poultry and pets in Portugal

Antimicrobial resistance and the mechanisms implicated in faecal enterococci from healthy humans, poultry and pets in Portugal

Quinupristin‐Dalfopristin Resistance in Gram‐Positive Bacteria: Mechanism of Resistance and Epidemiology

Crystal Structure of Vat(D): An Acetyltransferase That Inactivates Streptogramin Group A Antibiotics^,

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Occurrence of satA and vgb Genes in Streptogramin-Resistant Enterococcus faecium Isolates of Animal and Human Origins in The Netherlands

Cited by 68 publications

References 13 publications

Antimicrobial resistance and the mechanisms implicated in faecal enterococci from healthy humans, poultry and pets in Portugal

Antimicrobial resistance and the mechanisms implicated in faecal enterococci from healthy humans, poultry and pets in Portugal

Quinupristin‐Dalfopristin Resistance in Gram‐Positive Bacteria: Mechanism of Resistance and Epidemiology

Crystal Structure of Vat(D): An Acetyltransferase That Inactivates Streptogramin Group A Antibiotics,

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Crystal Structure of Vat(D): An Acetyltransferase That Inactivates Streptogramin Group A Antibiotics^,