Occurrence of antimicrobial resistance among bacterial pathogens and indicator bacteria in pigs in different European countries from year 2002 – 2004: the ARBAO-II study

Hendriksen, René S.; Mevius, Dik; Schroeter, Andreas; Teale, Christopher; Jouy, Éric; Butaye, Patrick; Franco, Alessia; Utinane, Andra; Amado, A.; Moreno, Miguel A.; Greko, Christina; Stärk, Katharina D.C.; Berghold, C; Myllyniemi, Anna-Liisa; Hoszowski, A.; Sunde, Marianne; Aarestrup, Frank Møller

doi:10.1186/1751-0147-50-19

Cited by 74 publications

(65 citation statements)

References 17 publications

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“…The growing interest in this emerging pathogen is reflected by recent whole-genome sequencing studies of selected isolates directed at elucidating the occurrence and evolution of the genetic determinants of its pathogenicity and drug resistance (7,18,43). High rates of tetracycline resistance have been reported in both human and pig isolates of S. suis (17,22,41,44). Before our report (23), tet(M) and tet(O) were the only tetracycline resistance determinants identified in S. suis (8,35,38,42); quite recently, tet(L) (18), mosaic tet(O/W/32/O) (25), and tet(W) itself (25,43) have also been reported.…”

Section: Tet(w)mentioning

confidence: 99%

Different Genetic Elements Carrying the tet (W) Gene in Two Human Clinical Isolates of Streptococcus suis

Palmieri

Princivalli

Brenciani

et al. 2011

Antimicrob Agents Chemother

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The genetic support for tet(W), an emerging tetracycline resistance determinant, was studied in two strains of Streptococcus suis, SsCA and SsUD, both isolated in Italy from patients with meningitis. Two completely different tet(W)-carrying genetic elements, sharing only a tet(W)-containing segment barely larger than the gene, were found in the two strains. The one from strain SsCA was nontransferable, and aside from an erm(B)-containing insertion, it closely resembled a genomic island recently described in an S. suis Chinese human isolate in sequence, organization, and chromosomal location. The tet(W)-carrying genetic element from strain SsUD was transferable (at a low frequency) and, though apparently noninducible following mitomycin C treatment, displayed a typical phage organization and was named ⌽SsUD.1. Its full sequence was determined (60,711 bp), the highest BLASTN score being Streptococcus pyogenes ⌽m46.1. ⌽SsUD.1 exhibited a unique combination of antibiotic and heavy metal resistance genes. Besides tet(W), it contained a MAS (macrolide-aminoglycoside-streptothricin) fragment with an erm(B) gene having a deleted leader peptide and a cadC/cadA cadmium efflux cassette. The MAS fragment closely resembled the one recently described in pneumococcal transposons Tn6003 and Tn1545. These resistance genes found in the ⌽SsUD.1 phage scaffold differed from, but were in the same position as, cargo genes carried by other streptococcal phages. The chromosome integration site of ⌽SsUD.1 was at the 3 end of a conserved tRNA uracil methyltransferase (rum) gene. This site, known to be an insertional hot spot for mobile elements in S. pyogenes, might play a similar role in S. suis. tet(W)is an emerging tetracycline resistance determinant whose host range, including Gram-positive, Gram-negative, aerobic, and anaerobic bacteria, is second only to that of tet(M) among ribosomal protection tet genes (26). tet(W) was first identified in the rumen anaerobe Butyrivibrio fibrisolvens (3), where it was associated with a transposable chromosomal element. It was subsequently detected in several other bacteria of the animal and human gastrointestinal tract, associated with either conjugative or nonconjugative elements (1, 5, 13, 14, 19, 29-31, 33, 37).In 2008 we first described tet(W) in Streptococcus suis, in a human isolate from a sporadic case of meningitis in Italy (23). S. suis, a major swine pathogen worldwide, is emerging as a zoonotic agent, the most common human clinical manifestations being meningitis and sepsis (20,39). Most cases of human S. suis infection have originated in Southeast Asia, where pig rearing is widespread, and large outbreaks have occurred in China (20). In industrialized countries human infections are rare, albeit probably underdiagnosed, and usually arise as sporadic cases. The growing interest in this emerging pathogen is reflected by recent whole-genome sequencing studies of selected isolates directed at elucidating the occurrence and evolution of the genetic determinants of its pathogenicity and dru...

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Section: Tet(w)mentioning

confidence: 99%

Different Genetic Elements Carrying the tet (W) Gene in Two Human Clinical Isolates of Streptococcus suis

Palmieri

Princivalli

Brenciani

et al. 2011

Antimicrob Agents Chemother

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“…This humanveterinary information gathering initiative introduced scientific rigour to the surveillance process, addressing many of the variables that could otherwise confound interpretation of data (Wallmann et al 2003). Within a veterinary framework, Hendriksen et al (2008aHendriksen et al ( , 2008b) surveyed resistance across Europe among bacterial pathogens and indicator bacteria for pigs and cattle. While their work did not incorporate harmonisation efforts into the data collection process, it did provide a positive step forward towards the creation of national surveillance schemes.…”

Section: Monitoring Programmes: Points To Considermentioning

confidence: 99%

Antimicrobial Drug Resistance

Martinez

Silley²

2010

Comparative and Veterinary Pharmacology

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This chapter provides an overview of our current understanding of the mechanisms associated with the development of antimicrobial drug resistance, international differences in definitions of resistance, ongoing efforts to track shifts in drug susceptibility, and factors that can influence the selection of therapeutic intervention. The latter presents a matrix of complex variables that includes the mechanism of drug action, the pharmacokinetics (PK) of the antimicrobial agent in the targeted patient population, the pharmacodynamics (PD) of the bacterial response to the antimicrobial agent, the PK/PD relationship that will influence dose selection, and the integrity of the host immune system. Finally, the differences between bacterial tolerance and bacterial resistance are considered, and the potential for non-traditional anti-infective therapies is discussed.

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“…S. suis is an important zoonotic pathogen that leads to economic losses and severe invasive diseases in humans, with meningitis being the most common clinical presentation [4,12,21]. It has been reported that 421 isolates of S. suis from clinically healthy sows in China exhibited high level resistance to commonly used antibiotics, including tetracycline, sulfisoxazole, clindamycin, erythromycin and trimethoprim/sulfamethoxazole [10,26,28]. Novel antibiotics can be developed, but the pathogens ultimately become resistant to such drugs.…”

Section: Introductionmentioning

confidence: 99%

Combined Antibacterial Activity of Phage Lytic Proteins Holin and Lysin from Streptococcus suis Bacteriophage SMP

Shi

Yan

et al. 2012

Curr Microbiol

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Development of novel antibacterial agents is required to control infection with multidrug-resistant Streptococcus suis. HolSMP and LySMP, the holin and lysin of S. suis serotype 2 bacteriophage, named SMP, are responsible for lysis of host cells and release of progeny phage. HolSMP and LySMP expressed in Escherichia coli BL21(DE3) exerted efficient activity at 37 °C, pH 5.2, with addition of 0.8 % β-mercaptoethanol. Lytic spectra of purified HolSMP, LySMP or HolSMP + LySMP mixture were investigated. HolSMP, exhibiting a narrow lytic spectrum, was effective against Staphylococcus aureus and Bacillus subtilis, which were insensitive to LySMP. Moreover, HolSMP was identified as a promising antibacterial agent which was able to extend the spectrum of LySMP. The data suggest that combined use of holin and lysin could be a candidate strategy for resolution of drug resistance.

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Occurrence of antimicrobial resistance among bacterial pathogens and indicator bacteria in pigs in different European countries from year 2002 – 2004: the ARBAO-II study

Cited by 74 publications

References 17 publications

Different Genetic Elements Carrying the tet (W) Gene in Two Human Clinical Isolates of Streptococcus suis

Different Genetic Elements Carrying the tet (W) Gene in Two Human Clinical Isolates of Streptococcus suis

Antimicrobial Drug Resistance

Combined Antibacterial Activity of Phage Lytic Proteins Holin and Lysin from Streptococcus suis Bacteriophage SMP

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