Occurrence of antibiotic resistance in bacteria isolated from seawater organisms caught in Campania Region: preliminary study

Abstract

BackgroundEnvironmental contamination by pharmaceuticals is a public health concern: drugs administered to humans and animals are excreted with urine or faeces and attend the sewage treatment. The main consequences of use and abuse of antibiotics is the development and diffusion of antibiotic resistance that has become a serious global problem. Aim of the study is to evaluate the presence of antimicrobial residues and to assess the antimicrobial resistance in bacteria species isolated from different wild caught seawater fish and fishery products.ResultsThree antibiotic substances (Oxytetracicline, Sulfamethoxazole and Trimethoprim) were detected (by screening and confirmatory methods) in Octopus vulgaris, Sepia officinalis and Thais haemastoma. All Vibrio strains isolated from fish were resistant to Vancomycin (VA) and Penicillin (P). In Vibrio alginolyticus, isolated in Octopus vulgaris, a resistance against 9 antibiotics was noted.ConclusionsWild caught seawater fish collected in Gulf of Salerno (Campania Region), especially in marine areas including mouths of streams, were contaminated with antibiotic-resistant bacteria strains and that they might play an important role in the spread of antibiotic-resistance.

Antibiotics and Antibiotic Resistance in Agroecosystems: State of the Science

“…In free-living wildlife, the occurrence of ARB is well documented (e.g., Allen et al, 2010;Greig et al, 2015;Janecko et al, 2015;Smaldone et al, 2014), but we know little about the effect of this resistance on wildlife health. Some have proposed that ingested ARB could affect the intestinal microbiomes of wildlife species, leading to changes in the functional properties of these bacterial communities and perhaps to health consequences for the animal (Gillings, 2015).…”

Enumeration of <em>Escherichia coli</em> and determination of <em>Salmonella</em> spp. and verotoxigenic <em>Escherichia coli</em> in shellfish (<em>Mytilus galloprovincialis</em> and <em>Ruditapes decussatus</em>) harvested in Sardinia, Italy

“…This highlights the role of shellfish as vehicle for several hazards that could result in products that are unsuitable to guarantee the safety of consumers, particularly if live bivalve molluscs are consumed raw or lightly cooked (Rubini et al , 2018 ; Sferlazzo et al , 2018 ). Bivalve molluscs transmitted illness can be either due to indigenous bacteria as Vibrio species (Smaldone et al , 2014 ; Leoni et al , 2016 ) or from non-indigenous bacteria, usually enteric bacteria, derived from fecal contamination (mostly Salmonella spp., E. coli VTEC , Shigella spp.) (Marceddu et al , 2017 ).…”

Antibacterial efficacy of silver nanoparticles and ethyl acetate’s metabolites of the potent halophilic (marine) bacterium, Bacillus cereus A30 on multidrug resistant bacteria

“…Our susceptibility test on selected pathogens against AgNPs revealed the resistance-deterioration of the multidrug resistance-gene of the parental/resistance species (that transfers the genetic information), like the antibiotic resistance property of various offsprings. Radha et al [24] have also reported that E. coli and P. aeruginosa were resistant against most antibiotics. Frank [62] has demonstrated that these challenging resistant pathogens were characterized to exterminate their virulence as well as to inhibit the respective enzymes by superimposing AgNPs and ethyl acetate extract of strain A30.…”

“…Then each antibiotic disc was evenly dispensed and lightly pressed onto the agar surface. The test antibiotics did immediately begin to diffuse outwards from the discs, creating a gradient of antibiotic concentration in the agar such that the highest concentration was found close to the disc with decreasing concentrations further away from the disc [24,25]. After an overnight incubation at 37 °C, the zone of inhibition was measured.…”