Occurrence of antibiotic resistance genes in landfill leachate treatment plant and its effluent-receiving soil and surface water

Zhang, Xiaohua; Xu, Yanbin; He, Xiaolin; Huang, Lu; Ling, Jiayin; Zheng, Li; Du, Qingping

doi:10.1016/j.envpol.2016.08.081

Cited by 102 publications

(23 citation statements)

References 51 publications

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“…Antimicrobial resistance in aquatic environments has been linked to fecal waste, which is a source of antimicrobial-resistant bacteria and residual antimicrobial compounds ( 15 , 16 ). Runoff from landfills and sludge applied to land can also transport antimicrobial-resistant bacteria and residual antimicrobial compounds to coastal systems ( 17 ). The presence of resistance genes and residual antimicrobial compounds has been shown to select for the evolution and survival of resistant bacteria ( 15 ).…”

Section: Introductionmentioning

confidence: 99%

Relationship between Rainfall, Fecal Pollution, Antimicrobial Resistance, and Microbial Diversity in an Urbanized Subtropical Bay

Powers

Wallgren

Marbach

et al. 2020

Appl Environ Microbiol

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Urbanized bays are vulnerable to fecal bacterial pollution and the extent of this pollution, in marine recreational waters, is commonly assessed by quantifying enterococci concentrations. Recent reports have questioned the utility of enterococci as an indicator of fecal bacterial pollution in subtropical bays impaired by non-point source pollution, and enterococci data alone cannot identify fecal bacterial sources (i.e., hosts). The purpose of this study was to assess relationships between rainfall, fecal bacterial pollution, antimicrobial resistance, and microbial diversity in an urbanized subtropical bay. Thus, a comprehensive bacterial source tracking (BST) study was conducted using a combination of traditional and modern BST methodologies. Findings show that rainfall was directly correlated with elevated enterococci concentrations, including the increased prevalence of Enterococcus faecium, although it was not correlated with an increase in the prevalence of antimicrobial-resistant strains. Rainfall was also correlated with decreased microbial diversity. By contrast, neither rainfall nor enterococci concentrations were directly correlated with the concentrations of three omnipresent host-associated fecal markers (i.e., human, canine, and gull). Notably, the human fecal marker (HF183) was inversely correlated with enterococci concentrations, signifying that traditional enterococci data alone is not an accurate proxy for human fecal waste in urbanized subtropical bays. IMPORTANCE The presence of human enteric pathogens, stemming from fecal pollution, is a serious environmental and public health concern in recreational waters. Accurate assessments of fecal pollution are therefore needed to properly assess exposure risks and guide water quality policies and practices. In this study, the absence of a direct correlation between enterococci and source-specific human and animal markers disputes the utility of enterococci as an indicator of fecal pollution. Moreover, the inverse correlation between enterococci and the human-specific HF183 marker indicates that recreational beach advisories, triggered by elevated enterococci concentrations, are a misleading and ineffective practice. This study clearly demonstrates that a multiparameter approach that includes the quantitation of host-specific markers, as well as analyses of microbial diversity, is a more effective means of assessing water quality in urbanized subtropical bays.

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Section: Introductionmentioning

confidence: 99%

Relationship between Rainfall, Fecal Pollution, Antimicrobial Resistance, and Microbial Diversity in an Urbanized Subtropical Bay

Powers

Wallgren

Marbach

et al. 2020

Appl Environ Microbiol

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“…The ARGs will neutralize the benefit antibiotics once given to human beings (Baker ; Hiltunen et al ). Compared with drinking water and rivers, there are more resistant genes and types existing in reclaimed water (Barker‐Reid et al ; Fahrenfeld et al ; Wang et al ), urban waste water, leachate treatment plant and treatment plants (Schwartz et al ; Shakibaie et al ; Luprano et al ; Tang et al ; Zhang et al ), livestock and fishing water (Chen et al ; Frey et al ; Xiong et al ; He et al ; Jia et al ; Cui et al ). ARGs like tetA , tetB , tetC , tetW , tetX , tetG , tetM , tetO , tetS , tetQ , tetP , tetG , sul 1, sul2 , sul3 , blaTEM , qnrA , AmpC , mefA , aacC1 , strA , ermB , cmlA5 , vanA , dfrA1 , qepA , oqxA , oqxB , cat , cml , bla , aacA , aph , aadA , mecA , intI1 , might contribute to the dissemination of antibiotic resistance among pathogens.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Microbiomes and Resistomes in Two Karst Groundwater Sites in Chongqing, China

Zhang¹,

Kuang²,

Xu³

et al. 2019

Groundwater

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Karst groundwater is an important water resource, as it accounts for about 15% of the total landscape of the earth and supplies 20% of potable water worldwide. The antibiotics resistance is an emerging global concern, and antibiotics residual and increase of antibiotic resistance genes represent serious global concerns and emerging pollutants. There is no report on the antibiotic resistance genes in groundwater. To survey resistome and microbiome in karst groundwater, two karst water samples were chosen for metagenome and metatranscriptome study, namely the 37th spring (C) and Dongcao spring (R) in Beibei, Chongqing, China. The two sites differ significantly in sulfur content, geochemical parameters, community structure, antibiotic resistance genes, and mechanisms, and these results may be influenced by anthropogenic activities. Combining with the Antibiotic Resistance Genes Database, three types of resistance genes baca, sul2, sul1 are present in R and C, and ant3ia, ermc, tetpa are also present in R. The number of all resistance genes in R was more than C, and Proteobacteria, Bacteroidetes, Nitrospirae are the main sources of antibiotic resistance genes. In addition, a large number of genes related to antibiotic gene transmission and drug resistance were found in both samples. Karst groundwater is an important source of drinking water and a possible venue for the transmission of microbial antibiotic resistance genes. However, few studies addressed this issue in karst groundwater, despite its widespread and great importance to global ecosystem. Karst groundwater is a reservoir for antibiotic resistant genes, and measures to control these resistant genes are urgently needed.

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“…Similarly, Wang et al [12], Wu et al [13] and Song et al [14] reported the detection of ARGs in the metagenomic DNA fraction of leachate and refuse from landfill sites in China. Some of these studies have reported a positive correlation between the presence of metals and occurrence of antibiotic resistance genes in the leachate emanating from the investigated landfills pointing to possible co-selection pressure in the landfill ecosystem [15,16]. While these studies contributed immensely to establishing landfills as important reservoir of antibiotic resistance, they did not provide information on the taxonomic identity of the bacteria host and/or the genetic context of detected ARG.…”

Section: Introductionmentioning

confidence: 99%

Class 1 Integron, Sulfonamide and Florfenicol Resistance Genes in Bacteria from Three Unsanitary Landfills, Ibadan, Nigeria

Adelowo

Osuntade²

2019

Journal of Microbiology and Infectious Diseases

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Objective: Municipal solid waste (MSW) landfills recently emerged as potential reservoirs of antibiotic resistance. However, little is known about the potentials of unsanitary landfill sites which are common in developing countries as reservoir of antibiotic resistance. In this study, we investigated the antibiotic resistance pattern and presence of selected resistance genes in bacteria from three MSW landfills in Ibadan, southwestern Nigeria. Methodology: Fifty six antibiotic-and metal-resistant bacteria isolated from leachate and leachate-contaminated surface and groundwater collected from three MSW landfills in Ibadan, Southwestern Nigeria, were searched for sulfonamides, florfenicol and beta-lactams resistance genes and integrons by PCR. Results: None of the 27 β-lactamase genes tested was detected in the isolates. sul1, sul2 and floR were however detected in 12 (21.4%), 2 (3.6%) and 12 (21.4%) bacteria species identified as Alcaligenes faecalis, Advenella kashmirensis, Brevundimonas spp. and Ralstonia pickettii. The 12 bacteria carrying sul1/floR gene combination were also positive for class 1 integron. Analysis of the gene cassettes in the variable regions of the class 1 integrons (>1.5kb) of the 12 intl1-positive isolates revealed the presence of aadB and unknown genes. The deduced amino acid sequence of the unknown portion of the gene cassettes shared 42-65% identity with sequences of Class D βlactamase and OXA-2-like protein in the GenBank. Conclusions: Findings of this study suggest that the landfill ecosystem is a potential site for the evolution of novel resistance genes and are hence important reservoir of antibiotic resistant bacteria.

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Occurrence of antibiotic resistance genes in landfill leachate treatment plant and its effluent-receiving soil and surface water

Cited by 102 publications

References 51 publications

Relationship between Rainfall, Fecal Pollution, Antimicrobial Resistance, and Microbial Diversity in an Urbanized Subtropical Bay

Relationship between Rainfall, Fecal Pollution, Antimicrobial Resistance, and Microbial Diversity in an Urbanized Subtropical Bay

Comparison of Microbiomes and Resistomes in Two Karst Groundwater Sites in Chongqing, China

Class 1 Integron, Sulfonamide and Florfenicol Resistance Genes in Bacteria from Three Unsanitary Landfills, Ibadan, Nigeria

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