Occurrence of anti-Neospora caninum and anti-Toxoplasma gondii antibodies in dogs with visceral leishmaniasis

Ribeiro, Raul Rio; Silva, Manoel E.; Silva, Sydnei M.; Fulgêncio, Gustavo O; Pena, Hilda Fátima Jesus; Frézard, Frédéric; Michalick, Marilene Suzan Marques; Gennari, Solange Maria

doi:10.1590/s0100-736x2011000600012

Cited by 5 publications

(3 citation statements)

References 39 publications

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“…It is important to note that the majority of the CCZ dogs were domiciled and not stray dogs, and the owner took them to be sacrificed due to leishmaniasis. This result is in agreement with studies from Gennari et al (2006) with dogs from Araçatuba, SP, and from Ribeiro et al (2011), with dogs from Belo Horizonte, that found, respectively, no association (p > 0.05) and a tendency of association (p = 0.053), between Leishmania chagasi positive dogs and occurrence of T. gondii infection.…”

Section: Resultssupporting

confidence: 92%

Occurrence of antibodies anti-Toxoplasma gondii and anti-Neospora caninum in dogs from Natal, RN, Brazil

Lopes

Hernández

Lima

et al. 2015

Braz. J. Vet. Res. Anim. Sci.

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The occurrence of anti-Toxoplasma gondii and Neospora caninum in dogs from the municipality of Natal, RN, Brazil, was determined. Information about the presence of these coccidia in this species was not known. Blood samples were collected from 29 domiciled dogs which inhabit areas that surround two important protected areas of Atlantic Forest (Parque da Cidade and Parque das Dunas) and another 73 dogs that were sacrificed due to Leishmania spp. infection, in Center for Control of Zoonosis (CCZ) for the municipality of Natal. It was only possible to obtain information about the gender of dogs that live in the parks area. The presence of antibodies against each parasite was determined by indirect Fluorescence Antibody Test (IFAT), with a cutoff of 16 for T. gondii and 50 for N. caninum. Of the 102 dogs examined, 13 (12.7%, 95% CI 7.0-20.8%) were T. gondii positive and three (2.9%, CI 0.6-8.4%) for N. caninum. Association between: localities of obtaining samples (parks x CCZ) and sex of animals, with the occurrence of antibodies against each of the parasites, was determined by the Fisher exact test. For T. gondii association was found with males (p = 0.027) and dogs living close to parks (p = 0.008) had higher rates of infection. Associations were not observed in relation to N. caninum.Keywords: Dogs. Toxoplasma gondii. Neospora caninum. Serology. Natal. ResumoA ocorrência de anticorpos anti-Toxoplasma gondii e anti-Neospora caninum foi determinada em cães do município de Natal, RN, onde informações sobre a presença destes coccídios nessa espécie não era conhecida. Para tanto, foram utilizados 29 cães domiciliados, que habitam áreas que circundam duas importantes Unidades de Conservação de Mata Atlântica presentes no município (Parque da Cidade e Parque das Dunas), e outros 73 cães que foram sacrificados no Centro de Controle de Zoonoses de Natal por serem positivos a Leishmania spp. Somente em relação aos cães que vivem próximos aos parques foi possível a obtenção de informações sobre o sexo dos animais. A presença de anticorpos contra cada um dos coccídios foi determinada com a Reação de Imunofluorescência Indireta com ponto de corte de 16 para T. gondii e 50 para N. caninum. Dos 102 cães examinados, 13 (12,7%, IC 95% 7,8) foram positivos para T. gondii e 3 (2,9%, IC 0,6-8,4%) para N. caninum. Associação entre: localidades de obtenção das amostras (parques x CCZ) e sexo dos animais com a ocorrência de anticorpos contra cada um dos parasitos, determinada através do teste exato de Fisher, foram positivas para T. gondii, com os machos (p = 0,027) e cães que habitam próximos aos parques (p = 0,008) apresentando maiores taxas de infecção. Associações não foram observadas em relação a N. caninum.

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Section: Resultssupporting

confidence: 92%

Occurrence of antibodies anti-Toxoplasma gondii and anti-Neospora caninum in dogs from Natal, RN, Brazil

Lopes

Hernández

Lima

et al. 2015

Braz. J. Vet. Res. Anim. Sci.

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“…This puppy contained CDV RNA by RT-PCR, with additional positive immunolabelling for CDV, CAdV-2 and CPV-2, resulting in a quadruple infection; five infectious disease agents including T. gondii and CDV were diagnosed in puppy 10 . Moreover, coinfections of Leishmania chagasi , N. caninum , and T. gondii have been investigated in dogs, where it was suggested that the immunodepressive effects of L. chagasi might have influenced infections by N. caninum and T. gondii 58 . Therefore, one wonders if the known immunodepressive effects of CDV 4 might have favoured the development of the protozoan infection in this puppy.…”

Section: Discussionmentioning

confidence: 99%

Canine morbillivirus (canine distemper virus) with concomitant canine adenovirus, canine parvovirus-2, and Neospora caninum in puppies: a retrospective immunohistochemical study

Headley

Oliveira

Pereira

et al. 2018

Sci Rep

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A retrospective immunohistochemical study was designed to investigate the frequency of concomitant traditional infectious disease pathogens in puppies that died suddenly and review the aspects of associated pathogenesis. Fifteen puppies were evaluated; the pathology reports and histopathologic slides of these animals were reviewed to determine the pattern of histopathologic lesions. The intralesional identification of antigens of canine (distemper) morbillivirus (CDV), canine adenovirus-1 and -2 (CAdV-1 and -2), canine parvovirus-2 (CPV-2), Toxoplasma gondii, and Neospora caninum was evaluated by IHC within the histopathologic patterns observed. All puppies contained CDV nucleic acid by molecular testing. The most frequent histopathologic patterns were intestinal crypt necrosis (n = 8), white matter cerebellar demyelination (n = 7), necrohaemorrhagic hepatitis (n = 7), interstitial pneumonia (n = 7), and gallbladder oedema (n = 5). All puppies contained intralesional antigens of CDV in multiple tissues resulting in singular (n = 3), and concomitant dual (n = 3), triple (n = 5) and quadruple (n = 4) infections by CAdV-1, and -2, CPV-2, and N. caninum; T. gondii was not identified. Concomitant infections by CDV was observed with N. caninum (100%; 1/1), CPV-2 (100%; 8/8), CAdV-1 (100%; 8/8), and CAdV-2 (100%; 8/8). Intralesional antigens of CDV and not CAdV-1 were identified in cases of gallbladder oedema. The “blue eye” phenomenon was histologically characterized by corneal oedema and degenerative lesions to the corneal epithelium, without inflammatory reactions.

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“…Recent Brazilian surveys of foxes found seropositivity for N. caninum, T. gondii and L. infantum in 3.2%, 18.0% and 78.1%, respectively (CATENACCI et al, 2009). Coinfection has already been described with L. infantum and N. caninum (TARANTINO et al, 2001;CRINGOLI et al, 2002;ANDREOTTI et al, 2006) and with these parasites and T. gondii in dogs (GENNARI et al, 2006;RIBEIRO et al, 2011). In addition, some studies suggest that immunosuppression caused by Leishmania spp.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence rates of antibodies againstLeishmania infantum and other protozoan and rickettsial parasites in dogs

Paulan

Lins

Tenório

et al. 2013

Rev. Bras. Parasitol. Vet.

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Canine visceral leishmaniasis (CVL) is caused by the protozoan Leishmania infantum, which infects dogs and humans in many regions of Brazil. The present study involved an indirect fluorescent antibody test (IFAT) to analyze L. infantum, Ehrlichia spp., Babesia canis, Toxoplasma gondii and Neospora caninum infection rates in serum samples from 93 dogs in a rural settlement in Ilha Solteira, SP, Brazil. The seroprevalence rates of anti-L. infantum, anti-Ehrlichia, anti-B. canis, anti-T. gondii and anti-N. caninum antibodies were 37.6%, 75.3%, 72%, 47.3% and 6.4%, respectively. In addition to IFAT, direct microscopic examination of popliteal lymph node aspirates revealed 26.9% of CVL positive dogs. Serological tests revealed that 17.2% of the dogs were seropositive for a single parasite, 29% for two parasites, 33% for three, 16.1% for four, and 1.1% for five parasites, while 3.2% were seronegative for five parasites. The presence of antibodies against these parasites in serum samples from dogs confirmed their exposure to these parasites in this rural area. Because of the potential zoonotic risk of these diseases, mainly leishmaniasis, ehrlichiosis and toxoplasmosis, special attention should focus on programs for the improvement of diagnostic assays and control measures against these parasites.

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Occurrence of anti-Neospora caninum and anti-Toxoplasma gondii antibodies in dogs with visceral leishmaniasis

Cited by 5 publications

References 39 publications

Occurrence of antibodies anti-Toxoplasma gondii and anti-Neospora caninum in dogs from Natal, RN, Brazil

Occurrence of antibodies anti-Toxoplasma gondii and anti-Neospora caninum in dogs from Natal, RN, Brazil

Canine morbillivirus (canine distemper virus) with concomitant canine adenovirus, canine parvovirus-2, and Neospora caninum in puppies: a retrospective immunohistochemical study

Seroprevalence rates of antibodies againstLeishmania infantum and other protozoan and rickettsial parasites in dogs

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