Occurrence of a stable nitrate reductase inActinidiaspp.

Abstract

Following development of an in vitro assay, a NADH/NADPH bispecific nitrate reductase stable for at least a month at 4°C, and thermostable at temperatures to at least 60°C, has been identified in crude and partially-purified extracts of leaves and roots of kiwifruit (Actinidia deliciosa). Stability characteristics were not confined to kiwifruit, but were found to extend to other members of the genus also.

Arginase from kiwifruit: Properties and seasonal variation

“…This brings into question just how the plant distributes and utilises its nitrogenous resources, and its impact on fruit quality. Apart from report of an unusual thermostable nitrate reductase extracted from the roots of kiwifruit (Godlewska & Clark 1996), the biochemistry of the enzymes associated with N assimilation and utilisation processes in kiwifruit have not been investigated.…”