Occurrence of a novel tannase (tan B LP ) in endophytic Streptomyces sp. AL1L from the leaf of Ailanthus excelsa Roxb

Abstract

The tannase production ability by endophytic actinobacteria and the genetic identity of responsible tannase gene were determined. The studied strains were isolated from surface-sterilized leaf discs of Roxb. Four strains were found to hydrolyze tannic acid on solid media containing 0.4% tannic acid. The strain AL1L was found as indicating production of tannase with diverse of substrate affinity. The tannase production from the potential strain AL1L was performed in liquid tannic acid broth (0.4%, w/v). The strain was later identified as sp. AL1L on the basis of 16S rDNA homology. Highest enzyme activity was observed at 48 h of incubation at the exponential growth phase. The enzyme was purified by ammonium sulfate precipitation followed by dialysis (15 kD cut off). This enzyme, with molecular weight 180 kD shows highest catalytic activity at 35 °C, pH 6 with substrate concentration 0.1 g%. The purified enzyme possesses 1.4 × 10 and 11.15 U/ml as. The above study indicates high industrial prospective of endophytic actinobacteria as source of tannase of potential biotechnological applications.

Production of Bioactive Compounds with Broad Spectrum Bactericidal Action, Bio-Film Inhibition and Antilarval Potential by the Secondary Metabolites of the Endophytic Fungus Cochliobolus sp. APS1 Isolated from the Indian Medicinal Herb Andrographis paniculata

“…Plants and their endophytes are co-evolutionary partners and thus share valuable genes. They are factories of novel bioactive compounds with unique biotechnological importance in the control, prevention and amelioration of fatal diseases by producing antimicrobial, antioxidative, and anticancer compound sand industrially valuable enzymes [ 4 , 5 , 6 , 7 , 8 ]. Hydrocarbon derivatives from endophytic sources with bio-fuel potency are popularly known as myco-diesel and provide a glimpse into the ecology of fungus–plant and environmental relationship [ 9 ].…”

Studies on the Effect of Bacterial Tannase Supplementation to Biodegradation of Tannins in Tannery Wastewater

“…Streptomyces sp. from leaf disks of Ailanthus excelsa Roxb showed 6.96 U/mL of activity at 30 °C, 48 h, 150 rpm …”

“…from leaf disks of Ailanthus excelsa Roxb showed 6.96 U/mL of activity at 30 °C, 48 h, 150 rpm. 25 Bacillus licheniformis exhibited 0.356 U/mL tannase activity at 40 °C, pH 5.0, 21 h, 200 rpm. 26 Pseudomonas aeroginosa from Jamun and keekar leaves showed 13.65 U/mL at 37 °C, pH 7.0, 24 h, 200 rpm.…”

Morphological and physiological response of sour orange (Citrus aurantium L.) seedlings to the inoculation of taxonomically characterized bacterial endophytes

“…DNA was extracted by the CTAB method ( Wilson, 1987 ). Total genomic DNA of ten bacterial strains was subjected to PCR using 16S rDNA primers (universal) 27F (5′ AGAGTTTGATCMTGGCTCAG 3′), 1492R (5′ ACCTTGTTACGACTT 3′) ( Roy et al, 2018 ), and previously reported PCR conditions were applied. 55 °C was used as annealing temperature for 16S rRNA universal Primers amplification and 1500 bp PCR Product was obtained as described by ( Mushtaq et al, 2021 ).…”