Occurrence of a Mucin-type Glycoprotein in Nidamental Gland Mucosubstance from the Squid &lt;i&gt;Illex argentinus&lt;/i&gt;

Kimura, Shigeru; Sugiura, Yoko; Mizuno, Haruo; Kato, Noboru; Hanaoka, Yutaka

doi:10.2331/fishsci.60.193

Cited by 8 publications

(4 citation statements)

References 1 publication

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“…Mucin-like proteins have also been reported in protozoans (16,17). A secretory mucin involved in squid egg mass formation was identified from the nidamental gland (18,19). A glycoprotein from Drosophila melanogaster cultured cells, was reported to be a mucin-like protein (20).…”

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confidence: 99%

Molecular Cloning and Sequencing of a Novel Invertebrate Intestinal Mucin cDNA

Wang¹,

Granados²

1997

Journal of Biological Chemistry

117

103

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The first invertebrate intestinal mucin, termed insect intestinal mucin (IIM), was recently identified from Trichoplusia ni larvae (Wang, P., and Granados, R. R. (1997) Proc. Natl. Acad. Sci. U. S. A., in press). We report the cDNA cloning and sequencing of IIM, which is only the second completely sequenced intestinal mucin after human intestinal mucin, MUC2. To clone and sequence the cDNA for IIM, a T. ni larval midgut cDNA expression library was constructed and screened with an anti-IIM antiserum. Two full-length cDNA clones for IIM were identified and sequenced. The deduced proteins from the two cDNA clones contained 807 and 788 amino acid residues, respectively. The structural organization of IIM is similar to that of MUC2, containing a 25-amino acid signal leading sequence and two threonine/proline/ alanine-rich tandem repeat domains flanked by cysteine-rich sequences. One tandem repeat domain contained two repeating units, TTTQAP and AATTP, and the other contained one repeating unit, TAAP. The cysteine-rich regions showed potential chitin binding features. By immunolocalization in tissue sections, it was determined that IIM is expressed in midgut tissues. The IIM mRNA is abundant in the midgut tissue, and Northern blot analysis indicated that IIM transcripts were not polydispersed as is found in mammalian mucin transcription.

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confidence: 99%

Molecular Cloning and Sequencing of a Novel Invertebrate Intestinal Mucin cDNA

Wang¹,

Granados²

1997

Journal of Biological Chemistry

117

103

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“…Furthermore, the ratio of L-Histidine was higher than that of the other amino acids. L-Histidine shows more fa-tigue-alleviation effects than other amino acids 13 . For example, imidazole dipeptides such a L-Carnosine or L-Anserine also exhibit L-Histidine residue and antioxidant activities.…”

Section: Analysis Of Amino Acids By Uhplcmentioning

confidence: 93%

Evaluation of Antioxidant Activity and Amino Acids in the Mucus of Mackerel for Cosmetic Applications

Tagami

Kuwahara

2020

J. Oleo Sci.

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IntroductionThe stratum corneum of the skin is located at the boundary between the inside of the skin and outside environment, functioning as a barrier against stimulation from the outside environment. When barrier function decreases, the water retained in the stratum corneum evaporates, leading to dry skin, which can cause inflammation and itching. This study is based on interview survey results that revealed improvement in symptoms such as atopic dermatitis and rough, itchy hands in people who had been handling mackerel for a long time. Previous studies of fish mucus reported the structure and functions of glycoprotein lectins, mucin, antioxidant, and antimicrobial peptides 1 4 . Glycoprotein, lectins, and mucin are high-molecular-weight materials, whereas antioxidants and antimicrobial peptides are low-molecular-weight materials. The mucus of fish is predominantly composed of the mucins of glycoprotein, whose amino acid compositions differ depending on the species 5 . Antioxidants and antimicrobial peptides are less susceptible to heat denaturation than proteins such as lectins and mucins.

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Section: Introductionmentioning

confidence: 99%

“…Chemically, the outer jelly of the egg mass is composed of a mucosubstance secreted by the nidamental gland [ 18 ]. The water-soluble mucin-type glycoprotein component of the mucosubstance is thought to generate the density and viscosity of the outer jelly of the egg mass [ 18 – 21 ]. The jelly also acts as a physical barrier between the eggs and the surrounding water, protecting the eggs from infestation [ 13 , 14 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Structure and properties of the egg mass of the ommastrephid squid Todarodes pacificus

et al. 2017

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The Japanese flying squid, Todarodes pacificus, is thought to spawn neutrally buoyant egg masses that retain a specific location in the water column by floating at the interface between water layers of slightly different densities. It is important to understand the physical process that determines the vertical distribution of the egg masses to predict their horizontal drift in relation to embryo survival and subsequent recruitment. Here, mesocosm experiments were conducted in a 300 m3 tank by creating a thermally stratified (17–22°C) water column to obtain egg masses. A cage net methodology was developed to sustain egg masses for detailed observation. We measured the density of the egg masses of T. pacificus, and used this information to infer the vertical distribution patterns of the egg masses at the spawning grounds (Tsushima Strait, Japan). When measured separately, the density of the outer jelly of each egg mass was 2.7 σ units higher than that of the surrounding water. The outer jelly and the specific gravity of embedded individual eggs (~1.10) cause the egg masses to have very slight negative buoyancy relative to the water in which they are formed. Analysis of the vertical profile of the spawning ground showed that water density (σθ) increased sharply at ~30 m depth; thus, egg masses might settle above the pycnocline layer. In conclusion, we suggest that T. pacificus egg masses might retain their location in the water column by floating at the interface between water layers of slightly different densities, which happen to be above the pycnocline layer (actual depth varies seasonally/annually) in the Tsushima Strait between Korea and Japan.

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Occurrence of a Mucin-type Glycoprotein in Nidamental Gland Mucosubstance from the Squid <i>Illex argentinus</i>

Cited by 8 publications

References 1 publication

Molecular Cloning and Sequencing of a Novel Invertebrate Intestinal Mucin cDNA

Molecular Cloning and Sequencing of a Novel Invertebrate Intestinal Mucin cDNA

Evaluation of Antioxidant Activity and Amino Acids in the Mucus of Mackerel for Cosmetic Applications

Structure and properties of the egg mass of the ommastrephid squid Todarodes pacificus

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