Occurrence of a monocyte/macrophage colony‐stimulating factor in the continuous ambulatory peritoneal dialysis fluids and its chromatographic behaviors and antigenicity compared with human urinary colony‐stimulating factor

Abstract

Continuous ambulatory peritoneal dialysis (CAPD) fluid from three patients with chronic renal failure exhibited the activity of colony-stimulating factor (CSF) in amounts varying from 5 to 40 units per ml. Like the CSF obtained from normal human urine, the peritoneal CSF predominantly produced monocyte/macrophage colonies in soft-agar culture of mouse bone marrow cells. Semipurified peritoneal CSF showed its isoelectric point at pH 3.6 and 4.9 before and after the treatment with neuraminidase. Under the same conditions, the urinary CSF was focused at pH 3.1 and 4.6. The position of elution of the peritoneal and urinary CSF in ordinary gel-filtration chromatography corresponded to a molecular weight of 62,000 and 117,000, whereas both CSFs exhibited a molecular weight of 28,000 upon gel-filtration in the presence of 6 M guanidine HCl. Furthermore, the two CSFs from the human sources were neutralized by antimouse L cell CSF serum in the same manner. We conclude that the peritoneal CSF is a sialoglycoprotein which is nearly identical with the urinary CSF despite processing of the latter through kidneys.

Human macrophage colony‐stimulating factor heterogeneity results from alternative mRNA splicing, differential glycosylation, and proteolytic processing

“…M-CSF protein has been purified to apparent homogeneity from both MIA PaCa-2 cells and human urine. In each case, the purified M-CSF protein was reported to consist of a single species of 23 to 28 kd on reduced SDS-PAGE [9][10][11][12][13][14]16,18]. However, about half of the starting M-CSF activity was lost during lectin affinity chromatography.…”

IV-1 Growth Factors

“…Colony-stimulating factors (CSFs) have been found and purified from body fluids s93 and cells, i.e., urine by Motoyoshi et al (1978), Yoshida sarcoma cells by Ohno, Seki and Shikita (1978), L-P3 cells by Tsuneoka and Shikita (1980), carrageenininduced inflammatory cells by Shikita et al (1981), ambulatory peritoneal dialysis fluid by Sakai, Tsuneoka and Shikita (1984), long-term bone marrow cultures by Mori's group (Miyanomae et al, 1982;Tsurusawa et al, 1983), and mammary carcinoma cells by Ayusawa et al (1979). As CSFs are glycoproteins, a CSF derivative is produced in the presence of tunicamycin as detected by Hozumi's group (Ayusawa et al, 1979;Yamamoto et al, 1981) and by Shikita's group .…”