Occurrence of a 16SrII-V Subgroup Phytoplasma Associated with Witches’-Broom Disease in <i>Melochia corchorifolia</i> in China

Yu, Shao-Shuai; Wu, Yuan; Song, Wei

doi:10.1094/pdis-07-21-1375-pdn

Cited by 4 publications

(7 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many plants could be infected by phytoplasmas belonging to different groups [ 27 , 29 , 30 ]. Trema tomentosa , showing witches’ broom disease, was infected by the phytoplasma strains belonging to the 16SrXXXII-D subgroup [ 29 ]; Melochia corchorifolia witches’ broom disease was caused by the phytoplasma strains belonging to the 16SrII group [ 27 ]; Waltheria indica virescence disease was associated with the 16SrI group phytoplasma strains [ 30 ]; areca palm yellow leaf disease was also induced by the phytoplasmas belonging to the 16SrI group [ 38 , 39 ]. Citrus Huanglongbing disease symptoms in citrus plants, such as Citrus maxima , Citrus sinensis and Citrus × limon , were also found on the island and were identified to be associated with the pathogen of Candidatus Liberibacter asiaticus [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…The plants of chinaberry, lettuce and mulberry could be infected by the phytoplasma strains belonging to the 16SrI group [ 14 ]. The plants of Melochia corchorifolia , Tephrosia purpurea and Emilia sonchifolia could be infected by the phytoplasma strains belonging to the 16SrII group [ 27 , 28 , 42 ]. From this study, it could be seen that the plants of Citrus maxima and Trema tomentosa could be infected by the phytoplasma strains belonging to the 16SrXXXII-D subgroup [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…Economic palm crops, such as areca palm, coconut and oil palm, were also planted on the farm. Moreover, there were many phytoplasma-infected plants reported previously, such as Melochia corchorifolia , Emilia sonchifolia , Trema tomentosa and Waltheria indica , which were also found on the farm [ 27 , 28 , 29 , 30 ]. Total DNA of the diseased plant samples was extracted based on the CTAB method, according to the rapid extraction kit for plant genomic DNA (Tiangen Biotech Co., Ltd., Beijing, China) [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

“…The disease symptoms of Citrus maxima are similar to the symptoms caused by Citrus Huanglongbing disease. Furthermore, some phytoplasma diseases were reported previously in the regions associated with the disease symptoms of Citrus maxima [ 27 , 28 , 29 , 30 ]. Therefore, the plants with disease symptoms were suspected to be associated with the pathogens of phytoplasma and Candidatus Liberibacter asiaticus, based on a field investigation and a previous study [ 17 , 18 , 19 , 23 , 27 , 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, some phytoplasma diseases were reported previously in the regions associated with the disease symptoms of Citrus maxima [ 27 , 28 , 29 , 30 ]. Therefore, the plants with disease symptoms were suspected to be associated with the pathogens of phytoplasma and Candidatus Liberibacter asiaticus, based on a field investigation and a previous study [ 17 , 18 , 19 , 23 , 27 , 28 , 29 , 30 ]. At present, there are no reports of mixed infection with phytoplasma and Candidatus Liberibacter asiaticus on the citrus plant hosts and even other plant hosts on Hainan Island in China.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Molecular Identification and Characterization of Two Groups of Phytoplasma and Candidatus Liberibacter Asiaticus in Single or Mixed Infection of Citrus maxima on Hainan Island of China

Zhu

Song

et al. 2022

Biology

Self Cite

View full text Add to dashboard Cite

The pathogens associated with citrus Huanglongbing symptoms, including yellowing and mottled leaves in Citrus maxima, an important economic crop on Hainan Island of China, were identified and characterized. In the study, detection, genetic variation and phylogenetic relationship analysis of the pathogens were performed based on 16S rRNA and β-operon gene fragments specific to phytoplasma and Candidatus Liberibacter asiaticus. The results indicated that the pathogens—such as phytoplasma strains of CmPII-hn belonging to the 16SrII-V subgroup and CmPXXXII-hn belonging to the 16SrXXXII-D subgroup, as well as Candidatus Liberibacter asiaticus strains CmLas-hn—were identified in the diseased plant samples, with numbers of 12, 2 and 6 out of 54, respectively. Among them, mixed infection with the 16SrII-V subgroup phytoplasma and Candidatus Liberibacter asiaticus was found in the study, accounting for 7.4% (four samples). The phytoplasma strains of CmPII-hn—Tephrosia purpurea witches’ broom, Melochia corchorifolia witches’ broom and Emilia sonchifolia witches’ broom—were clustered into one clade belonging to the 16SrII-V subgroup, with a 99% bootstrap value. The phytoplasma strains of CmPXXXII-hn and Trema tomentosa witches’ broom belonging to 16SrXXXII-D, and the other 16SrXXXII subgroup strains were clustered into one clade belonging to the 16SrXXXII group with a 99% bootstrap value. There were 16 variable loci in the 16S rRNA gene sequences of the tested 16SrXXXII group phytoplasma strains, of which two bases had an insertion/deletion. The strains of Candidatus Liberibacter asiaticus, identified in the study and the strains that had been deposited in GenBank, were in one independent cluster with a 99% bootstrap value. To our knowledge, this is the first report showing that Citrus maxima can be infected by 16SrII-V and16SrXXXII-D subgroup phytoplasmas in China. Moreover, this is also the first report in which the plants are co-infected by 16SrII-V subgroup phytoplasmas and Candidatus Liberibacter asiaticus. More comprehensive and detailed identification and characterization of the pathogens associated with the diseased symptoms in Citrus maxima on the island in China would be beneficial for epidemic monitoring and for the effective prevention and control of related plant diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular Identification and Characterization of Two Groups of Phytoplasma and Candidatus Liberibacter Asiaticus in Single or Mixed Infection of Citrus maxima on Hainan Island of China

Zhu

Song

et al. 2022

Biology

Self Cite

View full text Add to dashboard Cite

show abstract

First report of 16SrII-V phytoplasma associated with cowpea (Vigna unguiculata) phyllody in China

She

et al. 2023

Canadian Journal of Plant Pathology

View full text Add to dashboard Cite

The complete chloroplast genome sequence of Melochia corchorifolia Linnaeus, 1753 (Sterculiaceae)

Wang,

Wang

2024

Mitochondrial DNA Part B

View full text Add to dashboard Cite

Melochia corchorifolia Linnaeus, 1753, is a weedy tropical plant of the Sterculiaceae family and has medicinal value. We sequenced the complete chloroplast genome of M. corchorifolia using Illumina high-throughput sequencing and examined phylogenetic relationships with closely related species. The assembled chloroplast genome of M. corchorifolia was 163,693 bp long and contained a pair of inverted repeats of 29,729 bp, separated by a large single-copy sequence of 84,350 bp and a small single-copy region of 19,885 bp. A total of 136 genes were annotated across the entire chloroplast genome, including 37 transfer RNA, 8 ribosomal RNA, and 91 protein-coding genes. The GC content of the complete cp genome was 37.27%. The phylogenetic tree indicated that M. corchorifolia is closely related to Melochia pyramidata (Malvaceae). These results would provide useful information for future phylogenetic, taxonomic, and evolutionary studies on Sterculiaceae and Malvaceae.

show abstract

Occurrence of a 16SrII-V Subgroup Phytoplasma Associated with Witches’-Broom Disease in Melochia corchorifolia in China

Cited by 4 publications

References 3 publications

Molecular Identification and Characterization of Two Groups of Phytoplasma and Candidatus Liberibacter Asiaticus in Single or Mixed Infection of Citrus maxima on Hainan Island of China

Molecular Identification and Characterization of Two Groups of Phytoplasma and Candidatus Liberibacter Asiaticus in Single or Mixed Infection of Citrus maxima on Hainan Island of China

First report of 16SrII-V phytoplasma associated with cowpea (Vigna unguiculata) phyllody in China

The complete chloroplast genome sequence of Melochia corchorifolia Linnaeus, 1753 (Sterculiaceae)

Contact Info

Product

Resources

About