Occurrence, identification and pathogenicity of <i>Fusarium</i> species associated with guava wilt disease in India

Gangaraj, R.; Arumugam, Natarajan; Gaba, Swati; Das, Amrita; Prameeladevi,; Debbarma, Rubin; Choudhary, Shiv Pratap; Kumari, Anjali; Kamil, Deeba

doi:10.1080/03235408.2021.2005368

Cited by 10 publications

(4 citation statements)

References 17 publications

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“…The characteristics of the F. verticillioides colonies coincided with those described by Guo et al (2021); while, the features of microconidia and macroconidia were similar to those reported by Yilmaz et al (2021). Several studies have recorded F. verticillioides causing wilt symptoms in yams, root rot in tobacco and wilt in guava (Dongzhen et al 2020;Gai et al 2021;Gangaraj et al 2021); however, this is the rst report of F. verticillioides as the causal agent of chickpea wilt in Mexico and worldwide.…”

Section: Discussionsupporting

confidence: 81%

Phylogeny, distribution, and pathogenicity of fusarioid fungi associated with chickpea wilt in Sinaloa and Sonora, Mexico

Cota-Barreras¹,

García‐Estrada²,

León‐Félix³

et al. 2023

Preprint

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Wilt disease complex, is the most important disease of chickpeas (Cicer arietinum L.) in the production areas from Mexico. Disease symptoms include root rot, yellowing, wilting, poor growth, discoloration of vascular tissues, and death of plants. This study aimed to identify the fusarioid species associated with chickpea wilt in northwest Mexico by the combination of phylogenetic analyses and morphological characterization, as well as to determine their pathogenicity and virulence on chickpea seedlings. A total of 80 isolates of fusarioid fungi were obtained from symptomatic plants of 16 chickpea fields distributed in Sinaloa and Sonora, Mexico. Subsequently, a subset of 41 isolates representing the range of geographic origin was selected for further morphological characterization, phylogeny, and pathogenicity tests. Phylogenetic analyses of partial fragments of the translation elongation factor 1-alpha (tef1-α) and RNA polymerase second largest subunit (rpb2) genes were used to determine the identity of 26 Fusarium isolates and 15 Neocosmospora isolates to species level. Pathogenicity tests were performed on chickpea seedlings (cv. Blanco Sinaloa) under greenhouse conditions. Phylogenetic analyses of 41 fungal isolates of this study allowed the identification of Fusarium languescens (51.2%), Neocosmospora falciformis (36.6%), F. nirenbergiae (7.3%), and F. verticillioides (4.9%). All fungal isolates were found to be pathogenic on chickpea seedlings and a significant difference in virulence was observed. To our knowledge, F. languescens and F. nirenbergiae, belonging to the Fusarium oxysporum species complex, as well as F. verticillioides belonging to the Fusarium fujikuroi species complex are recorded for the first time as causal agents of chickpea wilt in Mexico and worldwide.

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Section: Discussionsupporting

confidence: 81%

Phylogeny, distribution, and pathogenicity of fusarioid fungi associated with chickpea wilt in Sinaloa and Sonora, Mexico

Cota-Barreras¹,

García‐Estrada²,

León‐Félix³

et al. 2023

Preprint

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“…Fusarium oxysporum f. sp. psidii strain (ITCC-8288) was deposited in the Indian Type Culture Collection (ITCC), New Delhi, and NCBI GenBank accession numbers (MN972593) were obtained (Gangaraj et al, 2022 ). In total, 11 strains of A. niger were collected, and isolated and pure cultures were obtained through the hyphal tip method by culturing on water agar medium, sub-cultured, and maintained on PDA slants at 26 ± 1°C ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…Among them, Fusarium oxysporum f. sp. psidii (FOP) is predominant, and its pathogenicity is also confirmed under field conditions (Gupta et al, 2010;Gangaraj et al, 2022). Since it is a soil-borne disease, once established in the field, it is very difficult to control.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic profiling and its association with the bio-efficacy of Aspergillus niger strain against Fusarium wilt of guava

et al. 2023

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Bio-control agents are the best alternative to chemicals for the successful management of plant diseases. The fungus Aspergillus niger is known to produce diverse metabolites with antifungal activity, attracting researchers to exploit it as a bio-control agent for plant disease control. In the present study, 11 A. niger strains were isolated and screened for their antagonism against the guava wilt pathogen under in vitro and in planta conditions. Strains were identified morphologically and molecularly by sequencing the internal transcribed spacer (ITS), β-tubulin, and calmodulin genes. The strains were evaluated through dual culture, volatile, and non-volatile methods under an in vitro study. AN-11, AN-6, and AN-2 inhibited the test pathogen Fusarium oxysporum f. sp. psidii (FOP) at 67.16%, 64.01%, and 60.48%, respectively. An in planta study was conducted under greenhouse conditions with 6 months old air-layered guava plants (var. Allahabad Safeda) by pre- and post-inoculation of FOP. The AN-11 strain was found to be effective under both pre- and post-inoculation trials. Furthermore, gas chromatography–mass spectrometry (GC–MS) analysis was carried out to characterize the volatile compounds of the most potential strain, A. niger. The hexane soluble fraction showed the appearance of characteristic peaks of hexadecenoic acid methyl ester (4.41%), 10-octadecanoic acid methyl ester (3.79%), dodecane (3.21%), undecane (3.19%), gibepyrone A (0.15%), 3-methylundecane (0.36%), and citroflex A (0.38%). The ethyl acetate fraction of the bio-control fungi revealed the occurrence of major antifungal compounds, such as acetic acid ethyl ester (17.32%), benzopyron-4-ol (12.17%), 1,2,6-hexanetriol (7.16%), 2-propenoic acid ethanediyl ester (2.95%), 1-(3-ethyloxiranyl)-ethenone (0.98%), 6-acetyl-8-methoxy dimethyl chromene (0.96%), 4-hexyl-2,5-dihydro dioxo furan acetic acid (0.19%), and octadecanoic acid (1.11%). Furthermore, bio-control abilities could be due to hyper-parasitism, the production of secondary metabolites, and competition for sites and nutrients. Indeed, the results will enrich the existing knowledge of metabolomic information and support perspectives on the bio-control mechanism of A. niger.

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“…Pathogenicity tests were only performed on Fusarium chlamydosporum and confirmed it as a pathogen of guava wilt. Recently, Gangaraj et al [59] reported Fusarium oxysporum f. sp. psidii and Fusarium falciforme as the causal pathogens of guava wilt in India.…”

Section: Wilt Diseasementioning

confidence: 99%

Fungal and Oomycete Diseases of Minor Tropical Fruit Crops

Zakaria

2022

Horticulturae

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Minor tropical fruits are grown on a small scale and provide income to smallholder farmers. The cultivation of these fruit crops indirectly contributes to the economy of producing countries as well as to food and crop security. Dragon fruits, guava, passionfruit, lychee, longan, mangosteen, durian, and rambutan are common minor fruit crops. In recent years, the international trade of some of these minor tropical fruits, particularly dragon fruit, passionfruit, guava, and lychee, has increased due to their nutritional value, with various health benefits. Similar to other crops, minor fruit crops are susceptible to fungal and oomycete diseases. These diseases negatively affect the yield and quality of fruit crops, leading to substantial losses. In this context, the knowledge of disease types and causal pathogens is fundamental to develop suitable disease management practices in the field as well as appropriate post-harvest treatments.

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Occurrence, identification and pathogenicity of Fusarium species associated with guava wilt disease in India

Cited by 10 publications

References 17 publications

Phylogeny, distribution, and pathogenicity of fusarioid fungi associated with chickpea wilt in Sinaloa and Sonora, Mexico

Phylogeny, distribution, and pathogenicity of fusarioid fungi associated with chickpea wilt in Sinaloa and Sonora, Mexico

Metabolomic profiling and its association with the bio-efficacy of Aspergillus niger strain against Fusarium wilt of guava

Fungal and Oomycete Diseases of Minor Tropical Fruit Crops

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