Occurrence, identification, and host range of Pectobacterium brasiliense causing soft rot on seed potato tubers in Turkey

Öztürk, Murat; Umar, Ahmet Resul

doi:10.1007/s41348-022-00675-8

Cited by 3 publications

(4 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is known that the genus Pectobacterium includes heterogonous strains characterized by diverse biochemical, physiological, and genetic properties even within the same species [ 54 ]. Based on the rep-PCR results, the Pectobacterium spp.…”

Section: Discussionmentioning

confidence: 99%

Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia

et al. 2023

View full text Add to dashboard Cite

The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Bačka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.

show abstract

Section: Discussionmentioning

confidence: 99%

Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Bacterial colonies were suspended in Luria–Bertani broth (LB; Merck, Darmstadt, Germany) and shaken at 180 rpm for 48 h. Genomic DNA was extracted from 1 mL of liquid culture's pelleted cells using a genomic DNA purification kit (GeneJET; Thermo Fisher Scientific) following the manufacturer's guidelines. The purity and concentration of extracted DNA were measured as described by Ozturk and Umar (2023).…”

Section: Methodsmentioning

confidence: 99%

“…For the identification of P. brasiliense, primers Br1f/L1r were employed (Duarte et al, 2004), and for P. parmentier, the PhF/R primer set was utilized (De Boer et al, 2012). PCR mixtures were prepared in a final volume of 50 μL, comprising 25 μL of 2 × master mix (MyTaq; Bioline, England), 2.5 μL of each primer (10 pmol), 17.5 μL of sterile dH 2 O, and 2.5 μL of template DNA (Ozturk & Umar, 2023). The PCR parameters were set as described by Aksoy, Kaya, et al (2017).…”

Section: Dna Extraction and Pcr Analysismentioning

confidence: 99%

Isolation, identification, and characterization of Pectobacterium actinidiae isolated from infected sugar beet leaves

Öztürk

2024

Journal of Phytopathology

View full text Add to dashboard Cite

During the sugar beet growing seasons of 2019 and 2020, symptomatic leaf samples displaying necrotic spots were collected for the isolation of pectinolytic bacteria. Seven bacterial isolates were obtained from these infected leaves on crystal violet pectate (CVP) medium. Two of these isolates exhibited the ability to macerate potato tuber slices and induced disease symptoms on petioles of sugar beet plants. Initially, their identity was determined to be Pectobacterium spp. through a polymerase chain reaction (PCR) that produced a 434 bp amplicon using Y1/Y2 primers. Subsequently, they generated a 550 bp PCR product with the EXPCCF/EXPCCR primers, classifying them as P. carotovorum. Further analysis of both strains involved the examination of the dnaX and mdh genes to confirm their taxonomic status. BLASTn analysis revealed the highest sequence similarity with P. actinidiae strains in GenBank for dnaX (99.39%–100%) and mdh (100%) genes. Phylogenetic analysis, based on sequences from dnaX, mdh, and a concatenation of both genes, conducted alongside 21 species of Pectobacterium spp., unequivocally confirmed that strains Pact1 and Pact2 belong to P. actinidiae. This study marks the first‐ever report of the presence of P. actinidiae in sugar beet worldwide.

show abstract

“…Initially suggested as a new subspecies of P. carotovorum, it was later classified as a separate species (Nabhan et al, 2012;Portier et al, 2019). P. brasiliense has recently gained worldwide attention as a significant threat to global potato production (Öztürk and Umar, 2022).…”

mentioning

confidence: 99%

First Report of Pectobacterium brasiliense Causing Bitter Melon Soft Rot Disease in Korea

Park,

Ten,

Hong

et al. 2023

Res. Plant Dis

View full text Add to dashboard Cite

In the Goesan region, located in Chungcheongbuk-do, Korea, a significant outbreak of soft rot infections was documented in August 2021, affecting fruits of Momordica charantia, commonly known as bitter melon or bitter gourd. The symptoms included a noticeable transition to yellowing in the affected fruits, eventually leading to their collapse. The bacterial strain KNUB-09-21 was isolated from the diseased fruits. Molecular analysis, using the sequences of the 16S rRNA region and three housekeeping genes (dnaX, recA, and leuS), along with the results of compound utilization in the API ID 32 GN system, provide strong evidence for the identification of the isolate KNUB-09-21 as Pectobacterium brasiliense. The pathogenicity of strain KNUB-09-21 on M. charantia was confirmed through a controlled inoculation test. Within two days, inoculated fruits displayed soft rot symptoms closely resembling those observed in naturally affected fruits. This is the first report of soft rot on M. charantia in Korea.

show abstract

Occurrence, identification, and host range of Pectobacterium brasiliense causing soft rot on seed potato tubers in Turkey

Cited by 3 publications

References 64 publications

Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia

Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia

Isolation, identification, and characterization of Pectobacterium actinidiae isolated from infected sugar beet leaves

First Report of Pectobacterium brasiliense Causing Bitter Melon Soft Rot Disease in Korea

Contact Info

Product

Resources

About