Occurrence, Distribution and Characterization of Rice Yellow Mottle Virus Isolates Genus Sobemovirus in Southwest Nigeria

Onasanya, R.O.; Olufolaji, D. B.; Onasanya, A.; Séré, Y.; Nwilene, F. E.; Wopereis, M.C.S.; Kiepe, Paul

doi:10.3923/tasr.2011.1301.1323

Cited by 5 publications

(8 citation statements)

References 12 publications

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“…Onasanya et al (2011) obtained similar results that showed that rymv 1-2 and rymv 1-3 were able to protect rice against Nigerian strains and only one strain out of 15 was able to attack TOG 5672 (rymv 1-4 and RYMV 2). Twenty strains collected in Nigeria (A. Oludare, unpublished data) and tested on the same accessions gave similar results (data not shown).…”

Section: Discussionmentioning

confidence: 66%

“…Twenty strains collected in Nigeria (A. Oludare, unpublished data) and tested on the same accessions gave similar results (data not shown). Onasanya et al (2011) obtained similar results that showed that rymv 1-2 and rymv 1-3 were able to protect rice against Nigerian strains and only one strain out of 15 was able to attack TOG 5672 (rymv 1-4 and RYMV 2). By opposition, other reports indicated that strains able to attack these accessions exist in Mali, Niger, Burkina Faso (Traor e et al 2006;Issaka et al 2012).…”

Section: Discussionmentioning

confidence: 66%

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Diversity of Rice yellow mottle virus in Benin and Togo and Screening for Resistant Accessions

Oludare

Tossou

Kini

et al. 2016

Journal of Phytopathology

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Surveys were conducted in rice fields in Benin, Cote d'Ivoire, Mali, Nigeria, Togo and Niger to assess the importance of Rice yellow mottle virus (RYMV). Diseased leaf samples were collected. In Togo, surveys were made mainly in the southern part of the country, and in Benin, all areas where rice is grown were covered. Leaf samples were serologically confirmed by ACP ELISA as bearing RYMV, propagated and all conserved. One hundred and forty‐eight (148) Beninese and 27 Togolese strains were serotyped. They were also phenotyped on three susceptible accessions including IR 64, four resistant lines with known alleles on RYMV 1 gene namely TOG 5681 (rymv 1‐3), TOG 5672 (rymv 1‐4 and RYMV 2), TOG 5674 (rymv 1.5) and Gigante (rymv 1‐2). RYMV spots with 9–100% incidences were identified. Serotyping by triple antibody sandwich (TAS) ELISA indicated that two main groups S1 and S2 coexisted in Benin with S1 being prevalent. In Togo, 26 strains were S1 and only one was S2. Phenotyping of the 148 Beninese and 27 Togolese indicated that they all attacked the three susceptible accessions, while TOG 5681 (rymv 1‐3), TOG 5672 (rymv 1‐4 and RYMV 2), TOG 5674 (rymv 1‐5) and Gigante (rymv 1‐2) remained symptomless. The use of the genes/alleles above in these countries against the disease is discussed. Three representative Beninese strains were selected to screen 48 accessions for disease resistance. Nine accessions were as susceptible as IR 64 to all strains while six (NERICA 9, NERICA 12, NERICA 13, TOG 7291, WAB56‐50, CG 14 and Moroberekan) were very resistant. Susceptibility of the six ARICA and resistance of some NERICA lines could be explained by the fact that one or both parental lines were, respectively, susceptible or resistant to the strains.

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Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 66%

Diversity of Rice yellow mottle virus in Benin and Togo and Screening for Resistant Accessions

Oludare

Tossou

Kini

et al. 2016

Journal of Phytopathology

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“…Discussion Viral disease due to RYMV had been a major challenge in rice production in Nigeria since 1976 when it was reported by Rossel et al (1982). Areas of rice production in Nigeria where the virus had been detected included Badeggi, Ibadan, Abeokuta, Alamala (Rossel et al, 1982;Onasanya et al, 2011) in Ogun State, but this is the first report of RYMV occurring on rice plants at Obafemi Owode and Ewekoro…”

Section: Resultsmentioning

confidence: 94%

“…During the two years survey, symptoms and severities varied in both locations alongside with incidence. The incidence of RYMV observed by Onasanya et al 2011 on farmer's field ranged between 15-70%. This is in contrast to this study as an incidence of 7.7% was observed in 2012 at Ewekoro while the highest incidene of 90.0 % was noticed in 2013 at Obafemi Owode LGA.…”

Section: Resultsmentioning

confidence: 99%

Prevalence of Rice Yellow Mottle Virus (RYMV) on Rice Plants Grown in Selected Farms in Ogun State: Preliminary Results

Odedara¹,

Ademolu²,

Ayo-John³

2016

Nig. J. Biotechnol

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Incidence of Rice yellow mottle virus (RYMV) on rice plants (ofada) grown in two local government areas (LGAs) of Ogun State had been evaluated during a two year field survey. Six month old rice plants were observed for symptom expression and leaf samples collected for serological indexing. Of the 60 leaf samples collected for both years, 96.7 % were presented with symptoms and severity ranged from 1 to 5 in 2012. Symptoms of brown blotches, chlorosis, leaf yellowing and necrosis were observed on rice plants from which samples were collected at Ewekoro LGA. Similar symptoms were also seen on rice plants at Obafemi Owode. Disease incidence due to RYMV was 7.7 % at Ewekoro in 2012 while it was 13.3 % for plants surveyed at Obafemi owode in the same year. In 2013, incidence varied from 30.0 to 90.0 % and the higher incidence was observed on samples at Obafemi owode. Serodiagnosis of Grasshopper (Zonocerus variegatus) and Locust (Locusta migratoides) trapped at the rice fields during the survey revealed that they were not implicated in virus transmission as their absorbance values at spectrophotometric wavelength of 405 nm were not up to one and half times the values of the healthy controls.Keywords: rice, ELISA, incidence, virus symptom, survey Correspondence: sdara@hotmail.com IntroductionWith increase in world population, there is emphasis on increasing level of food production, among which is rice. Rice (Oryza spp.) is grown widely in many parts of the world. It is the major food source for about 40 % of the world's human population (Ortiz, 2011) Production of rice in Nigeria is being threatened by infections from various pathogens such as Rice yellow mottle virus (RYMV) and others such as Maize streak virus (MSV) (Alegbejo, 2013). The virus, Rice yellow mottle virus (RYMV) is a non-enveloped isometric virus belonging to the genus Sobemovirus. Its particle size is 25 nm in diameter with conspicuous capsomere arrangement. The biochemical properties of RYMV include its virions containing 23 % nucleic acid, 77 % protein and 0 % lipid. It is a RNA virus, single stranded and its total genome size is 4.2 kb (kilobase).

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“…In Nigeria, RYMV has being the most prevalent causing a major challenge in rice production since 1976 when it was first reported (Rossel et al, 1982). Earlier studies done in the Southern part of Nigeria show the incidence of RYMV is up to 70% (Onasanya et al, 2011;Odedara et al, 2016). The adverse effect of viruses on rice production in Nigeria as well as limited information and literature on rice viruses in Abuja, necessitated this research with the objective to evaluate the incidence and severity of RYMV and MSV infecting rice and identify insects associated with rice production in Abuja.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the incidence and severity of rice yellow mottle virus and maize streak virus on rice (Oryza sativa L.) and associated insects in the Federal Capital Territory, Abuja, Nigeria

Folarin¹,

Asala²,

Oyerinde³

et al. 2020

Afr. J. Plant Sci.

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Rice is an important staple food inNigeria, affected by several diseases especially viruses. This study was carried out to evaluate the incidence and severity of two important viruses on rice plants and to identify associated insects in the Federal Capital Territory (F.C.T), Abuja in 2019. Field experiment was carried out from June to October, 2019 at the Teaching and Research Farm of the Faculty of Agriculture, University of Abuja, Nigeria, where ten rice varieties were assessed for incidence and severity. The seed and leaf samples were collected for serological indexing. Data collected was subjected to statistical analysis using SPSS and mean separation was done with Duncan Multiple Range Test. Of the 210 leaf samples collected, FARO 61 and 44 had the lowest incidence (19%), while FARO 65 recorded the highest (25.3%). FARO 52 recorded the highest severity (46%) while FARO 61 and FARO 60 had the lowest severity (30.1%) for rice yellow mottle sobemo-virus (RYMV) and maize streak geminivirus (MSV). Insects such as Spittle bug (Locris rubens and Poophilus costalis), Ladybird beetle (Cheilomenes sulphurea) and Groundhopper (Paratettix sp) were trapped on the field. All rice seed and leaf samples collected did not test positive to RYMV and MSV using Enzyme-Linked immunosorbent assay (ELISA). This study provides the first research work on rice viruses in the FCT and further studies are recommended.

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Occurrence, Distribution and Characterization of Rice Yellow Mottle Virus Isolates Genus Sobemovirus in Southwest Nigeria

Cited by 5 publications

References 12 publications

Diversity of Rice yellow mottle virus in Benin and Togo and Screening for Resistant Accessions

Diversity of Rice yellow mottle virus in Benin and Togo and Screening for Resistant Accessions

Prevalence of Rice Yellow Mottle Virus (RYMV) on Rice Plants Grown in Selected Farms in Ogun State: Preliminary Results

Evaluating the incidence and severity of rice yellow mottle virus and maize streak virus on rice (Oryza sativa L.) and associated insects in the Federal Capital Territory, Abuja, Nigeria

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