Occurrence and Potential Significance of Increased Numbers of T Cells with Fc Receptors in Myeloma<sup>1</sup>

Hoover, R G; Gebel, Howard M.; Dieckgraefe, Brian K.; Hickman, Scot; Rebbe, Neil F.; Hirayama, Norio; Óváry, Zoltán; Lynch, Richard G.

doi:10.1111/j.1600-065x.1981.tb01049.x

Cited by 77 publications

(18 citation statements)

References 22 publications

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“…More recently, Yodoi et al (10,11) reported that IgE induces expression of FcE receptors and production of IgE-suppressive factors by T cells. Similarly, on Lyt-2.3-positive T cells, Fca receptor can be induced either in vivo or in vitro by using purified monoclonal IgA (12).…”

mentioning

confidence: 99%

Isotype regulation of antibody production: T-cell hybrids can be selectively induced to produce IgG1 and IgG2 subclass-specific suppressive immunoglobulin-binding factors.

Löwy¹,

Brézin²,

Néauport–Sautès³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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T2D4, a T-cell hybrid, spontaneously secretes suppressive immunoglobulin factor(s); when incubated with purified monoclonal mouse immunoglobulins, this hybrid produces high levels of immunoglobulin-binding factors specific for the subclass of the inducing immunoglobulin. Thus, we were able to induce the production of IgGI-or IgG2-specific inhibitory factors by the same T2D4 T-cell hybrid. These subclass-specific suppressive factors bind selectively to the IgGI or IgG2 subclasses and inhibit specifically the secretion of antibodies of the corresponding subclass. Our results favor a model of negative regulation of isotype expression in which a given isotype triggers suppressor mechanism(s) specifically inhibiting its production.It has been established that B cells are not precommitted for the secretion of a given isotype (1, 2). In some experiments, 106 spleen cells from DNP-Ova-immunized animals were used, and results were similar. pfc were measured on day 5. IBF-containing samples were added at various dilutions at the initiation of culture. In some instances the suppressor effects were also tested in a cooperative response between poly(Glu,Ala,Tyr)-primed lymph node cells and DNPprimed B cells as described (6).IgGI, IgG2a, IgG2b, and IgA pfc Assays. Hemolytic plaque assays were performed as described (6). IgGI 2323The publication costs ofthis article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisenent" in accordance with 18 U. S. C. §1734 solely to indicate this fact.

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mentioning

confidence: 99%

Isotype regulation of antibody production: T-cell hybrids can be selectively induced to produce IgG1 and IgG2 subclass-specific suppressive immunoglobulin-binding factors.

Löwy¹,

Brézin²,

Néauport–Sautès³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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“…It has also been shown in mice bearing an IgE hybridoma that large numbers of T cells with Fc re ceptors for IgE (TeFcR cells) are induced [8;Hoover et al,in preparation]. Infection with Nippostrongylus brasiliensis could produce enrichment of lymphoid cells with receptors for IgE [9], In vitro exposure to monoclonal IgE also produced an induction of these receptors on lymphoid cells [9], Receptors for IgE in rats and humans are well documented [reviewed in 10]; however, this is not relevant to our study.…”

Section: Discussionmentioning

confidence: 99%

Studies on Immunity in Hybridoma-Bearing Mice. A. Immune Response to Antigens

Hirano¹,

Óváry²

1984

Int Arch Allergy Immunol

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When immunized with ovalbumin (OVA), mice bearing subcutaneously injected anti-dinitrophenyl (DNP) IgE-secreting hybridoma cells do not produce anti-OVA IgE antibodies as long as anti-DNP IgE is present in abundance in their sera. As soon as the titer of anti-DNP IgE antibody falls, anti-OVA IgE antibody appears. This inhibition of production of anti-OVA IgE is attributed to Fc_ε-bearing T cells which have a suppressive action of limited duration. Other isotypes of anti-OVA antibodies are produced in similar amounts as in controls which were immunized with OVA but not injected with hybridoma cells.

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“…It has been shown that Lyt-2+ FcR (receptor for Fc) cells appear in mice injected with myelomas or hybridomas and that the FcR cells are specific for the isotype of the immunoglobulin secreted by the in jected myeloma or hybridoma [21]. These T cells have the phenotype of suppressor T cells and it has been suggested that they function as isotype-specific sup pressor T cells [21].…”

Section: Suppression By T Cellsmentioning

confidence: 99%

Studies on Immunity in Hybridoma-Bearing Mice A. Immune Response to Antigens

Yin¹,

Furusawa²,

Hirano³

et al. 1987

Int Arch Allergy Immunol

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When BALB/c mice, which had rejected the anti-dinitrophenyl (DNP) IgE-producing hybridoma B 53, were immunized with DNP proteins, they produced much less anti-DNP antibodies than control (normal) mice. The anti-DNP plaque-forming cell (PFC) number was much less when spleen cells from mice immunized with DNP proteins were treated with sera of mice which had rejected the hybridoma B 53 than the PFC number from the same spleen cells not treated by the sera. The sera of mice which had rejected the hybridoma B 53 contained an inhibitor which was adsorbed and eluted from an anti-mouse immunoglobulin column and also a mouse anti-DNP IgG2a column. The inhibition of PFC was hapten-reversible. In Western blotting the eluates from the anti-DNP IgG2a column reacted as well with the blotted anti-DNP IgE B 53 as an anti-idiotypic antibody to anti-DNP IgE B 53. These criteria establish that the inhibitor in the sera of the mice which had rejected the B 53 tumor was an anti-idiotypic antibody of the type which mimics the epitope (DNP) of the immunizing antigen.

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Occurrence and Potential Significance of Increased Numbers of T Cells with Fc Receptors in Myeloma¹

Cited by 77 publications

References 22 publications

Isotype regulation of antibody production: T-cell hybrids can be selectively induced to produce IgG1 and IgG2 subclass-specific suppressive immunoglobulin-binding factors.

Isotype regulation of antibody production: T-cell hybrids can be selectively induced to produce IgG1 and IgG2 subclass-specific suppressive immunoglobulin-binding factors.

Studies on Immunity in Hybridoma-Bearing Mice. A. Immune Response to Antigens

Studies on Immunity in Hybridoma-Bearing Mice A. Immune Response to Antigens

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Occurrence and Potential Significance of Increased Numbers of T Cells with Fc Receptors in Myeloma1

Cited by 77 publications

References 22 publications

Isotype regulation of antibody production: T-cell hybrids can be selectively induced to produce IgG1 and IgG2 subclass-specific suppressive immunoglobulin-binding factors.

Isotype regulation of antibody production: T-cell hybrids can be selectively induced to produce IgG1 and IgG2 subclass-specific suppressive immunoglobulin-binding factors.

Studies on Immunity in Hybridoma-Bearing Mice. A. Immune Response to Antigens

Studies on Immunity in Hybridoma-Bearing Mice A. Immune Response to Antigens

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Occurrence and Potential Significance of Increased Numbers of T Cells with Fc Receptors in Myeloma¹