Occurrence and persistence of Listeria spp. in the environment of ewe and cow's milk cheese dairies in Portugal unveiled by an integrated analysis of identification, typing and spatial–temporal mapping along production cycle

Chambel, Lélia; Sol, Manuela; Fernandes, Isabel; Barbosa, Maria Luisa; Zilhão, Isabel; Barata, Belarmino; Jordan, Suzanne J.; Perni, Stefano; Shama, Gilbert; Adrião, Andreia; Faleiro, Maria Leonor; Requena, Teresa; Peláez, Carmen; Andrew, Peter W.; Tenreiro, Rogério

doi:10.1016/j.ijfoodmicro.2006.12.035

Cited by 69 publications

(42 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pulsed-Field Gel Electrophoresis (PFGE) and Random Amplification of Polymorphic DNA (RAPD) subtyping showed that the clinical isolate was indistinguishable from those collected from the processing plant and that closely related isolates persisted for five months in the dairy (Gianfranceschi et al, 2006). Different subtyping methods proved that specific isolates of L. monocytogenes are able to establish persistent contaminations in dairy premises for months and sometimes even years (Chambel et al, 2007;Leite et al, 2006;Rückerl et al, 2014). The mechanisms leading to the persistence are poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Biofilm Formation and Disinfectant Susceptibility of Persistent and Nonpersistent Listeria monocytogenes Isolates from Gorgonzola Cheese Processing Plants

Costa

Bertolotti

Brito

et al. 2016

Foodborne Pathogens and Disease

View full text Add to dashboard Cite

The aim of this study was to investigate whether the biofilm-forming ability and/or the disinfectant susceptibility accounted for the persistence of Listeria monocytogenes in Gorgonzola cheese processing plants. For this purpose, a set of 16 L. monocytogenes isolates collected in the 2004-2007 period was analyzed, including 11 persistent isolates collected in different years, within the collection period, and displaying identical or highly correlated pulsotypes. The evaluation of biofilm-forming ability was assessed using crystal violet (CV) staining and the enumeration of viable cells on stainless steel coupons (SCC). Absorbance values obtained with CV staining for persistent and non-persistent isolates were not significantly different (rm-ANOVA p>0.05) and the cell counts from non-persistent isolates showed to be higher than of persistent isolates (rm-ANOVA p<0.05). A simulation of disinfectant treatments was performed on spot inoculated coupons in clean and dirty conditions, according to EN 13697, and on biofilms on SSC, grown in nutrient-rich (dirty) and limiting (clean) conditions using acid acetic-hydrogen peroxide (P3) and acid citric-hydrogen peroxide (MS) commercial disinfectants. The treatment was considered effective when a 4 Log reduction in viable cell count was observed. The Log reductions of persistent and non-persistent isolates, obtained with both the assays in clean and dirty conditions, were compared and no significant differences were detected (rm-ANOVA p>0.05). A greater influence of organic matter on MS could explain why P3 was efficient in reducing to effective levels the majority of the isolates at the lowest concentration suggested by the manufacturer (0.2% [v/v]), while the same purpose required an higher concentration (1% [v/v]) of MS.In conclusion, our results demonstrate that the persistence of these isolates in Gorgonzola cheese processing plants was linked neither to the biofilm forming ability nor to their susceptibility to hydrogen peroxide-based disinfectants, therefore other factors should contribute to the persistent colonization of the dairies.

show abstract

Section: Introductionmentioning

confidence: 99%

Biofilm Formation and Disinfectant Susceptibility of Persistent and Nonpersistent Listeria monocytogenes Isolates from Gorgonzola Cheese Processing Plants

Costa

Bertolotti

Brito

et al. 2016

Foodborne Pathogens and Disease

View full text Add to dashboard Cite

show abstract

“…Researches on the presence of L. monocytogenes on the surface of equipment and utensils, report its occurrence in meat and dairy processing industries (11,15,27). According to Chae et al.…”

mentioning

confidence: 99%

Biofilm formation by Listeria monocytogenes on stainless steel surface and biotransfer potential

Oliveira¹,

Brugnera²,

Alves³

et al. 2010

Braz. J. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Therefore, three oligomer primers which had been successfully applied in other studies were used simultaneously. The primers were M13 (5′-GAG GGT GGC GGT TCT-3′) [57], Eric2 (5′-AAG TAA GTG ACT GGG GTG AGC G-3′) [58] and inlA.F (5′-CAG GCA GCT ACA ATT ACA CA-3′) [25]. These primers address different genomic regions: M13 is directed to sections containing micro-or mini-satellites, Eric.2 focuses repeated regions and inlA.F targets internalin genes [25].…”

Section: Rapd Typing Of Listeria Sppmentioning

confidence: 99%

“…The primers were M13 (5′-GAG GGT GGC GGT TCT-3′) [57], Eric2 (5′-AAG TAA GTG ACT GGG GTG AGC G-3′) [58] and inlA.F (5′-CAG GCA GCT ACA ATT ACA CA-3′) [25]. These primers address different genomic regions: M13 is directed to sections containing micro-or mini-satellites, Eric.2 focuses repeated regions and inlA.F targets internalin genes [25]. RAPD PCR reactions were performed in a final volume of 25 µl containing 50-100 ng of template DNA, 3 µl of 10 × PCR Buffer IV (Thermo Fisher Scientific, Dreieich, Germany), 1 mM dNTP Mix (Thermo Fisher Scientific), 3.5 mM MgCl 2 , 6.25 pmol/µl of each primer and 0.5 U of Thermoprime Plus Taq Polymerase (Thermo Fisher Scientific).…”

Section: Rapd Typing Of Listeria Sppmentioning

confidence: 99%

“…Also, ready-to-eat food or dairy products that have not been pasteurized, such as raw milk cheese or smoked salmon pose significant risks to consumers. Due to its importance as a food-borne pathogen, numerous studies on this genus deal with the occurrence and tracing of L. monocytogenes in food processing plants and in farm environments [17,[25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Permanent colonization of creek sediments, creek water and limnic water plants by fourListeriaspecies in low population densities

Lang-Halter

Schober

Scherer

2016

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

During a 1-year longitudinal study, water, sediment and water plants from two creeks and one pond were sampled monthly and analyzed for the presence of Listeria species. A total of 90 % of 30 sediment samples, 84 % of 31 water plant samples and 67 % of 36 water samples were tested positive. Generally, most probable number counts ranged between 1 and 40 g were detected. Species differentiation based on FT-IR spectroscopy and multiplex PCR of a total of 1220 isolates revealed L. innocua (46 %), L. seeligeri (27 %), L. monocytogenes (25 %) and L. ivanovii (2 %). Titers and species compositions were similar during all seasons. While the species distributions in sediments and associated Ranunculus fluitans plants appeared to be similar in both creeks, RAPD typing did not provide conclusive evidence that the populations of these environments were connected. It is concluded that (i) the fresh-water sediments and water plants are year-round populated by Listeria, (ii) no clear preference for growth in habitats as different as sediments and water plants was found and (iii) the RAPD-based intraspecific biodiversity is high compared to the low population density.Keywords: biodiversity; fresh water; Listeria monocytogenes; Listeria spp.; sediment; water plants.Dedication: This work is dedicated to the memory of Professor Peter Böger. His continuous support and thoughtful guidance over many years encouraged the senior author to aim at an academic career in microbial ecology and evolutionary biology.

show abstract

Occurrence and persistence of Listeria spp. in the environment of ewe and cow's milk cheese dairies in Portugal unveiled by an integrated analysis of identification, typing and spatial–temporal mapping along production cycle

Cited by 69 publications

References 54 publications

Biofilm Formation and Disinfectant Susceptibility of Persistent and Nonpersistent Listeria monocytogenes Isolates from Gorgonzola Cheese Processing Plants

Biofilm Formation and Disinfectant Susceptibility of Persistent and Nonpersistent Listeria monocytogenes Isolates from Gorgonzola Cheese Processing Plants

Biofilm formation by Listeria monocytogenes on stainless steel surface and biotransfer potential

Permanent colonization of creek sediments, creek water and limnic water plants by fourListeriaspecies in low population densities

Contact Info

Product

Resources

About