Occurrence and molecular analysis of extended-spectrum β-lactamase-producing Proteus mirabilis in Hong Kong, 1999–2002

Ho, Pak‐Leung; Ho, Alex Yat-Man; Chow, Kin-Hung; Wong, Chi Fong; Duan, Ran; Ho, Wai-Yew; Mak, Gannon C.K.; Tsang, K. T.; Yam, Wing Cheong; Yuen, Kwok‐Yung

doi:10.1093/jac/dki135

Cited by 34 publications

(24 citation statements)

References 42 publications

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“…A diversity of ESBLs such as TEM-derived mutants was reported to be produced by P. mirabilis in Europe (Bonnet et al, 1999;Luzzaro et al, 2001;Biendo et al, 2005). CTX-M-2-, CTX-M-3-, CTX-M-13-and CTX-M-14-producing P. mirabilis has been reported in Hong Kong, Taiwan and Japan (Nagano et al, 2003;Ho et al, 2005;Wu et al, 2006). These data indicate a geographical feature of ESBL-producing P. mirabilis, with TEM types in Europe and CTX-M types in Eastern Asia.…”

Section: Discussionmentioning

confidence: 99%

“…Uniquely, the species isolated in Japan possessed the CTX-M-2 enzyme frequently and predominantly in the Enterobacteriaceae. Previous studies have reported that ESBL-producing P. mirabilis in hospitals always encoded additional b-lactamase genes (Ho et al, 2005;Wu et al, 2006), with the exception of nosocomial outbreaks by ESBL-producing clonal strains (Nagano et al, 2003;Kim et al, 2004). …”

Section: Discussionmentioning

confidence: 99%

“…Since the 1990s, P. mirabilis producing TEM-derived ESBLs (TEM-15, TEM-24, TEM-52 and TEM-66) has been reported (Bonnet et al, 1999;Luzzaro et al, 2001;Biendo et al, 2005). Non-TEM-derived ESBLs such as VEB-1 (Kim et al, 2004), CTX-M b-lactamases (CTX-M-1, CTX-M-2, CTX-M-13 and CTX-M-31) (Ho et al, 2005;Karapavlidou et al, 2005) and plasmid-borne AmpC blactamases (CMY-2 and CMY-4) (Verdet et al, 1998;Park et al, 2006) have also been reported infrequently. The prevalence of ESBL-positive strains has been reported at 6.9 % in France , 7.0 % in Argentina (Quinteros et al, 2003), 8.8 % in Italy (Luzzaro et al, 2001) and 9.5 % in the US (Saurina et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Regional outbreak of CTX-M-2 β-lactamase-producing Proteus mirabilis in Japan

Nakano

Abe

et al. 2012

Journal of Medical Microbiology

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Proteus mirabilis is a common cause of urinary tract infection. Wild-type P. mirabilis strains are usually susceptible to penicillins and cephalosporins, but occurrences of P. mirabilis producing extended-spectrum b-lactamases (ESBLs) have been recently reported. Here, we surveyed the prevalence of cefotaxime resistance among P. mirabilis strains at seven different hospitals in Kanagawa Prefecture, Japan, and investigated their molecular epidemiology to explain the mechanism of their spread. The prevalence of cefotaxime resistance among P. mirabilis increased annually, from 10.1 % in 1998 to 23.1 % in 2003, and increased drastically in 2004, exceeding 40 %. We collected 105 consecutive and non-duplicate cefotaxime-resistant P. mirabilis isolates (MIC 16 to .256 mg ml "1 ) from these hospitals from June 2004 to May 2005 and characterized their profile. PCR and sequence analysis revealed that all resistant strains produced exclusively CTX-M-2 b-lactamase. PFGE analysis identified 47 banding patterns with 83 % or greater similarity. These results indicated that a regional outbreak of P. mirabilis producing CTX-M-2 blactamase has occurred in Japan and suggest that the epidemic spread occurred within and across hospitals and communities by extended clonal strains. Plasmid analysis revealed that 44.8 % of plasmids harboured by bla CTX-M-2 isolates had common profiles, encoding ISEcp1, IS26 and Int1, and belonged to incompatibility group T. Spread of the resistant isolates in Japan resulted from dissemination of narrow-host-range plasmids of the IncT group encoding bla CTX-M-2 . These findings indicate the rapidly developing problem of treating the species to prevent dissemination of ESBL producers.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regional outbreak of CTX-M-2 β-lactamase-producing Proteus mirabilis in Japan

Nakano

Abe

et al. 2012

Journal of Medical Microbiology

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“…Class A β-lactamases from the TEM group have occurred at a particularly high frequency; in many surveillance studies, they have been identifi ed as the resistance determinants most frequently encountered (2)(3)(4)(5)(6)(7)(8)(9). The fi rst bla TEM allele, bla TEM-1 , is a plasmidic allele that was fi rst isolated in 1963 (10,11).…”

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confidence: 99%

High Rate of Mobilization forbla_CTX-Ms

Barlow

Reik

Jacobs

et al. 2008

Emerg. Infect. Dis.

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We constructed a phylogenetic analysis of class A β-lactamases and found that the bla CTX-M s have been mobilized to plasmids ≈10 times more frequently than other class A β-lactamases. We also found that the bla CTX-M s are descended from a common ancestor that was incorporated in ancient times into the chromosome of the ancestor of Kluyvera species through horizontal transfer. Considerable sequence divergence has occurred among the descendents of that ancestral gene sequence since that gene was inserted. That divergence has mainly occurred in the presence of purifying selection, which indicates a slow rate of evolution for bla CTX-M s in the pre-antimicrobial drug era.

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“…An obvious prediction for ESBL bla TEM alleles is that they would either co-occur with bla TEM-1 or replace it as the most frequently encountered allele in environments where cephalosporins are heavily used. However, neither of these patterns has been observed among clinical populations of microorganisms, and bla TEM-1 is still the most commonly occurring allele in many microbial populations where cephalosporin resistance has been selected for (1,3,9,12,13,17,19,20,30,34). The high frequency of bla TEM-1 in microbial populations is counterintuitive, because the alleles descended from bla TEM-1 confer both the advantageous cephalosporin resistance phenotypes and the ancestral penicillin resistance phenotype, which should promote their fixation in microbial populations.…”

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confidence: 99%

Fitness Trade-Offs inbla_TEMEvolution

Mroczkowska

Barlow

2008

Antimicrob Agents Chemother

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bla TEM-1 expression results in penicillin resistance, whereas expression of many bla TEM-1 descendants, called extended-spectrum ␤-lactamases (ESBLs), results simultaneously in resistance to penicillins and extendedspectrum cephalosporins. Despite the expanded resistance phenotypes conferred by many ESBLs, bla TEM-1 is still the most abundant bla TEM allele in many microbial populations. This study examines the fitness effects of the two amino acid substitutions, R164S and E240K, that have occurred repeatedly among ESBL bla TEM-1 descendants. Using a single-nucleotide polymorphism-specific real-time quantitative PCR method, we analyzed the fitness of strains expressing bla TEM-1 , bla TEM-10 , and bla TEM-12 . Our results show that bacteria expressing the ancestral bla TEM-1 allele have a fitness advantage over those expressing either bla TEM-10 or bla TEM-12 when exposed to ampicillin. This observation, combined with the fact that penicillins are the most prevalent antimicrobials prescribed worldwide, may explain why bla TEM-1 has persisted as the most frequently encountered bla TEM allele in bacterial populations.

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Occurrence and molecular analysis of extended-spectrum β-lactamase-producing Proteus mirabilis in Hong Kong, 1999–2002

Abstract: Our findings indicate the emergence of CTX-M enzymes among P. mirabilis in Hong Kong. More ESBL screening of this species is required to improve their recognition.

Cited by 34 publications

References 42 publications

Regional outbreak of CTX-M-2 β-lactamase-producing Proteus mirabilis in Japan

Regional outbreak of CTX-M-2 β-lactamase-producing Proteus mirabilis in Japan

High Rate of Mobilization forbla_CTX-Ms

Fitness Trade-Offs inbla_TEMEvolution

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Occurrence and molecular analysis of extended-spectrum β-lactamase-producing Proteus mirabilis in Hong Kong, 1999–2002

Abstract: Our findings indicate the emergence of CTX-M enzymes among P. mirabilis in Hong Kong. More ESBL screening of this species is required to improve their recognition.

Cited by 34 publications

References 42 publications

Regional outbreak of CTX-M-2 β-lactamase-producing Proteus mirabilis in Japan

Regional outbreak of CTX-M-2 β-lactamase-producing Proteus mirabilis in Japan

High Rate of Mobilization forblaCTX-Ms

Fitness Trade-Offs inblaTEMEvolution

Contact Info

Product

Resources

About

High Rate of Mobilization forbla_CTX-Ms

Fitness Trade-Offs inbla_TEMEvolution