Occurrence and Mechanism of Exocytosis in Adrenal Medulla and Sympathetic Nerve

Winkler, Hans

doi:10.1007/978-3-642-46625-0_2

Cited by 29 publications

(12 citation statements)

References 446 publications

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“…These cells have served as a biological model for the study of the stimulus-secretion coupling process of neural tissues because the mechanisms involved in secretion, the released products and their neuroectodermic origin are similar to those of adrenergic neurons (Winkler 1988;Ungar and Phillips 1983;Borges 1997).…”

Section: Introductionmentioning

confidence: 99%

Morphological and functional characterization of beige mouse adrenomedullary secretory vesicles

Borges

Jaén

Freire

et al. 2001

Cell and Tissue Research

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We tested whether the giant secretory granules observed in the mast cells of the naturally occurring mutant beige mouse (BM) (C57BL/6N-bg) were also present in the adrenal chromaffin cells. The presence of large chromaffin granules (CG) would be a valuable tool for the study of exocytosis in neuronal tissues. Conversely, the observation of large vesicles within chromaffin cells that are different from CG could indicate that CG are of a different origin than granules of mast cells. Ultrastructural analysis demonstrated the presence of large lysososmal-like vesicles in the BM, and also a discrete increase in the number of CG with diameters larger than 240 nm but not of giant CG. In addition, amperometric measurements of single-event exocytosis, using carbon fiber microelectrodes, showed no differences between the quantal size of secretory events from BM and wildtype or bovine chromaffin cells. Minor but significant differences were found between the kinetics of exocytosis in BM cells andwild-type mouse cells. We conclude that CG, but not the abnormal-sized vesicles found in BM chromaffin cells contribute to the catecholamine secretion and that abnormal secretory granules are not present in adrenergic cell lineage.

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Section: Introductionmentioning

confidence: 99%

Morphological and functional characterization of beige mouse adrenomedullary secretory vesicles

Borges

Jaén

Freire

et al. 2001

Cell and Tissue Research

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“…Finally, one membrane receptor can be assigned directly to the A, subclass on the basis of the rank-order potency in the binding of different ligands; the main distinctive characteristic with respect to A, or A, is the similar potency displayed by R-PIA and NECA in the A, system Sebastiao, 1986, 1988). The granular content of catecholamines and ATP is released from chromaffin cells during exocytosis (Burgoyne, 1984;Winkler, 1988). Inhibition of catecholamine secretion from chromaffin cells by ATP, ADP, and adenosine has been described (Chern et al, 1987).…”

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confidence: 99%

The Adenosine Receptors Present on the Plasma Membrane of Chromaffin Cells Are of the A_2b Subtype

Casadó¹,

Casillas

Mallol³

et al. 1992

Journal of Neurochemistry

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The adenosine receptors in the plasma membrane of chromaffin cells from bovine adrenal medulla were characterized. The presence of A1 receptors was discounted owing to the absence of R-[3H]phenylisopropyladenosine (R-PIA) and [3H]8-cyclopentyl-1,3-dipropylxanthine ([3H]-DPCPX) binding. The binding of the specific A2a ligand CGS-21680 was low. In contrast, the binding of 5'-(N-[3H]-ethylcarboxamidoadenosine ([3H]NECA) was relatively high (1.7 pmol/mg of protein at a ligand concentration up to 90 nM). This binding did not correspond to non-adenosine receptor NECA binding sites because the specific [3H]-NECA binding was similar when unlabeled adenosine, NECA, or R-PIA was used to measure the nonspecific binding. The rank order of potency of different ligands for the displacement of specific [3H]NECA binding was DPCPX greater than NECA greater than chloroadenosine greater than R-PIA greater than theophylline = CGS-21680. These results indicate that the receptors present on the plasma membrane of chromaffin cells are exclusively of the A2b subtype.

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“…Depolarisation of the nerve cell plasma membrane causes an influx of calcium into the nerve terminal, with subsequent fusion of the vesicle with the plasma membrane and exocytosis of the content of the vesicles into the junction between the nerve terminal and the smooth muscle cell. The mechanism of exocytosis has recently been reviewed by Winkler (1988). The released noradrenaline then activates a-and P-adrenoceptors in the membrane of the smooth muscle cell (postjunctional adrenoceptors) or adrenoceptors located in the nerve terminal plasma membrane (prejunctional adrenoceptors) (see Adrenoceptors).…”

Section: The Sympathetic Neurovascular Junctionmentioning

confidence: 99%

Accumulation and release of adrenaline, and the modulation by adrenaline of noradrenaline release from rabbit blood vessels in vitro

Abrahamsen

1991

Pharmacology & Toxicology

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The accumulation of (-)-3H-adrenaline (3H-A) by rabbit isolated aorta was studied. In all experiments, monoamine oxidase and catechol-O-methyltransferase were inhibited by treatment with pargyline and 3',4'-dihydroxy-2-methyl-propiophenone, respectively. The relationship between the accumulation of 3H derived from 3H-A and the duration of incubation was linear. The 3H-accumulation after 3 h incubation was 22.5 ml/g. In reserpine-treated tissue, the 3H-accumulation levelled off after 30 min and was 8.5 ml/g after 3 h. The concentration of 3H-A or (-)-3H-noradrenaline (3H-NA) and the 3H-accumulation (ml/g) were inversely related. At 10(-8) M, the 1-hour accumulation of 3H derived from 3H-A and 3H-NA was 7.8 and 15.2 ml/g, respectively. With increasing concentrations the accumulation values approached each other. The accumulation of 3H derived from 3H-A by reserpine-treated tissue also showed an inverse relationship with concentration. The accumulation of 3H derived from 3H-A was dependent on the bath temperature. Storage of tissue (0-5 days in salt solution without equilibration with 95% O2/5% CO2; 4 degrees C) did not affect the accumulation of 3H derived from 3H-A. Thereafter (7-14 days), the accumulation decreased. The inhibitory potency (IC50; -log M) of desipramine, cocaine, propranolol, isoprenaline, and normetanephrine on accumulation of 3H derived from 3H-A was found to be 8.26; 6.50; 5.48; 4.88, and 4.02, respectively. The maximal degree of inhibition was almost the same for these drugs, while that of clonidine and corticosterone was 50 and 20%, respectively. In the presence of desipramine, either clonidine, corticosterone or isoprenaline reduces the accumulation of 3H derived from 3H-A. Ouabain and iodoacetic acid, but not sodium cyanide and 2,4-dinitrophenol, reduced the accumulation of 3H derived from 3H-A. Anoxia (95% N2/5% CO2; 37 degrees C; 1-24 h) did not alter the accumulation of 3H derived from 3H-A. Glucose deprivation alone or combined with anoxia markedly reduced the 3H-accumulation. The release of 3H-A from rabbit isolated aorta was studied. This release was compared with that of 3H-NA. The stimulation-evoked 3H-overflow from aorta preloaded with 3H-A decreased with repeated stimulation. In contrast, prestimulation enhanced subsequent stimulation-evoked 3H-overflows. For both 3H-amines, the 3H-overflow increased concomitantly to the same degree with the number of pulses. The time course of 3H-overflows with either 3H-A or 3H-NA was compared.(ABSTRACT TRUNCATED AT 400 WORDS)

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Occurrence and Mechanism of Exocytosis in Adrenal Medulla and Sympathetic Nerve

Cited by 29 publications

References 446 publications

Morphological and functional characterization of beige mouse adrenomedullary secretory vesicles

Morphological and functional characterization of beige mouse adrenomedullary secretory vesicles

The Adenosine Receptors Present on the Plasma Membrane of Chromaffin Cells Are of the A_2b Subtype

Accumulation and release of adrenaline, and the modulation by adrenaline of noradrenaline release from rabbit blood vessels in vitro

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Occurrence and Mechanism of Exocytosis in Adrenal Medulla and Sympathetic Nerve

Cited by 29 publications

References 446 publications

Morphological and functional characterization of beige mouse adrenomedullary secretory vesicles

Morphological and functional characterization of beige mouse adrenomedullary secretory vesicles

The Adenosine Receptors Present on the Plasma Membrane of Chromaffin Cells Are of the A2b Subtype

Accumulation and release of adrenaline, and the modulation by adrenaline of noradrenaline release from rabbit blood vessels in vitro

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The Adenosine Receptors Present on the Plasma Membrane of Chromaffin Cells Are of the A_2b Subtype