Occurrence and Location of Endophytic Bacteria in Garden and Wild Strawberry

Abstract

<jats:p>The occurrence of bacteria in different tissues was studied using field-grown strawberries, in vitro-grown strawberries, wild strawberries, and aseptically germinated strawberry seedlings. Strawberry has a number of endophytic bacteria in its the internal tissue, most of which appear to be nonpathogenic. In the in vitro-grown strawberries, all identified isolates were in the genus <jats:italic>Pantoea</jats:italic>. In field-grown garden and wild strawberries the most common genera were <jats:italic>Pantoea</jats:italic> and <jats:italic>Pseudomonas.</jats:italic> Location of eubacterial inhabitants within strawberry tissue sections was studied by in situ hybridization. Bacteria were detected in flower stalks, leaf stalks, leaves, stolons, berries and aseptically germinated seedlings. The existence of bacteria in seeds and seedlings suggests that bacteria are able to move up to the generative tissue and, ultimately, to the next generation, forming a symbiosis-like chain of plant-bacteria coexistence.</jats:p>

Papaya shoot tip associated endophytic bacteria isolated from in vitro cultures and host–endophyte interaction in vitro and in vivo

“…Pantoea spp. formed the most frequently isolated papaya endophyte as observed with citrus (Araújo et al 2002) and strawberry (Kukkurainen et al 2005). Pantoea has been isolated from other plant sources like grapevine (Bell et al 1995), rice (Feng et al 2006), and banana (P. Thomas, unpublished results).…”

Diversity of the spinach (Spinacia oleracea) spermosphere and phyllosphere bacterial communities

“…Numerous studies report the presence of seed microorganisms but few have examined their presence of these microbes post germination (Mundt & Hinkle, ; Cankar et al ., ; Kukkurainen et al ., ; López‐López et al ., ). In this study, pyrosequencing of 16s rRNA gene amplicons was used to examine the bacterial communities of spinach seeds and aerial tissues of spinach after germination (cotyledons) and plants (3–4 leaf stage).…”

“…The seed coat, embryo and endosperm of diverse crops such as alfalfa (Charkowski et al ., ), Norway spruce ( Picea abies L. H. Karst) (Cankar et al ., ), cereals and cucurbits (Mundt & Hinkle, ) yield culturable bacteria. Vertical transmission of bacteria from seed to germinated seedlings is reported in various species of Eucalyptus (Ferreira et al ., ), strawberry ( Fragaria × ananassa Duchesne) (Kukkurainen et al ., ), beans (López‐López et al ., ), rice (Cottyn et al ., ), Norway spruce (Cankar et al ., ) and tomato ( Lycopersicon esculentum L.) (Guo et al ., ).…”

Endophytic Bacillus and Pseudomonas spp. Modulate Apple Shoot Growth, Cellular Redox Balance, and Protein Expression Under in Vitro Conditions

“…However, several studies have shown that bacterial endophytes are common in plant tissues grown in vitro and that their beneficial effects on plant growth indicate that they may be useful as growth-promoting agents. In previous studies, a succession of bacterial communities that colonized pineapple microplant organs in vitro were characterized ( Abreu-Tarazi et al, 2010 ) Similarly, endophytic bacteria were isolated from strawberry tissues cultivated in vitro ( Kukkurainen et al, 2005 ; Dias et al, 2009 ), and their beneficial effect on the acclimatization of the seedlings under greenhouse conditions was demonstrated ( Dias et al, 2009 ). Recently, the effects of bacterial endophytes in different in vitro culture phases and in different plant organs of Prunus avium were studied; isolates of the endophytes Rhodopseudomonas palustris and Microbacterium testaceum promoted rooting in two difficult-to-propagate genotypes ( Quambusch et al, 2016 ).…”