Occurrence and Localization of Phycoerythrin in Symbiotic Nostoc of <i>Cycas revoluta</i> and in the Free-Living Isolated <i>Nostoc</i> 7422

Lindblad, Peter; Bergman, Birgitta

doi:10.1104/pp.89.3.783

Cited by 15 publications

(3 citation statements)

References 18 publications

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“…Our data are in contradiction with those of an earlier investigation using the small New Zealand species Gunnera albocarpa as test organism (Silvester 1976), but they are in agreement with the situation in other cyanobacterial symbioses. This means that phycobiliproteins have been found in representatives of all cyanobacterial-plant symbioses, from lichens to Gunnera (Bergman and Hallbom 1982, Lindblad et al 1985, Kaplan et al 1986, Perraju et al 1986, Bergman and Rai 1989, Lindblad and Bergman 1989). The potential for Nostoc in Gunnera to carry out oxygenic photosynthesis is obviously not diminished due to lack of antenna proteins.…”

Section: Discussionmentioning

confidence: 99%

The Nostoc-Gunnera magellanica symbiosis: Phycobiliproteins, carboxysomes and Rubisco in the cyanobiont

Söderbäck¹,

Bergman²

1992

Physiol Plant

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1992. The Nostoc-Gunnera magellanica symbiosis: Phycobiliproteins, carboxysomes and Rubisco in the cyanobiont. -Physioi. Plant. 84: 425-432.In the nitrogen fixing symbiosis between Nostoc and ttie angiosperm Gunnera. the eyanobiont is found in stem glands and is thought to have a heterotropbic mode of nutrition. To investigate whether the pbotosynthetic machinery in the cyanobiont is down-regulated in the symbiosis, the presence of the pbycobiliproteins, phycoerythrin and phyeocyanin, and ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco. EC 4.1.1.39) in cyanobionts of Gunnera magellanica Lam. and in a free-hving (cultured) isolate of tbe cyaoobacterium was studied by immunoelectron microscopy. Carboxysomes were numerous in all vegetative cells (ca 3.5 per ceil section), and on an area basis they showed a iiigh Rubisco label compared to tlie cytoplasm: bot recalculation on a volume basis demonstrated tbat the carboxysomal fraction of Rubisco decreased in the cyanobiont along the plant stem. Along the whole Gunnera stem both types of pbycobiliproteins were present in the symbiotic Nostoc and in amounts equivalent to or above those detected in the free-living isolate. As the symbiotic Nostoc is located intracellularly, out of reach of light in the plant stem, the findings indicate a lack of regulation of the pholosyntbetic protein synthesis in the symbiotic state.

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Section: Discussionmentioning

confidence: 99%

The Nostoc-Gunnera magellanica symbiosis: Phycobiliproteins, carboxysomes and Rubisco in the cyanobiont

Söderbäck¹,

Bergman²

1992

Physiol Plant

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“…Haselkorn (Chicago University, USA), Dr R. Oelmuller (Freiburg University, Germany) and Dr D. Guard-Friar (NY State Department of Health, USA), respectively. These antibodies have been tested, by western blotting, against a variety of cyanobacteria, including lichen cyanobionts, and have been found to be monospecific (Orr & Haselkorn, 1982;Lindblad & Bergman, 1986;Braun-Howland et al, 1988;Lindblad & Bergman, 1989;Bergman & Rai, 1989). We tested these antibodies against Calothrix sp.…”

Section: Antibodies Used and Their Specificitymentioning

confidence: 99%

“…These antibodies have been tested, by western blotting, against a variety of cyanobacteria, including lichen cyanobionts, and have been found to be monospecific (Orr & Haselkorn, 1982;BraunHowland et al, 1988;Lindblad & Bergman, 1989;. We tested these antibodies against Calothrix sp.…”

Section: Antibodies Used and Their Specificitymentioning

confidence: 99%

The marine lichen Lichina confinis (O. F. Müll.) C. Ag.: ultrastructure and localization of nitrogenase, glutamine synthetase, phycoerythrin and ribulose 1, 5‐bisphosphate carboxylase/oxygenase in the cyanobiont

1993

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SUMMARYThallus ultrastructure and the localization of proteins involved in photosynthesis, N^ fixation and animonia assimilation was studied in the Lichina confinis (O. F. Mull) C. Ag.-Calothrix symbiosis. The lichen thallus showed profuse branching. Within this, the cyanobiont was located in the cortical zone. Haustoria were frequently observed in contact with vegetative cells of the cyanobiont but not with heterocysts. An extensive mucilage layer containing numerous vesicles occurred around cyanobiont cells. Immunolabelling showed nitrogenase to be exclusively located in heterocysts of the cyanobiont and the free-living Calothrix sp. Glutamine synthetase (GS) levels in the cyanobiont were found to be drastically decreased compared to the levels in free-living (cultured) Calothrix sp. Such a decrease was more prominent in mature parts of the thallus than in the apical region. Rubisco was mostly located in carboxysomes of the vegetative cells of the cyanobiont. There were relatively low levels of Rubisco in tbe cytoplasm of vegetative cells, wbile heterocysts sbowed negligible levels. In free-living (cultured) Calothrix sp., pbycoerytbrin (PE) was found to be located along tbe tbylakoid membranes both in vegetative cells and beterocysts. Cyanobiont cells sbowed a similar pattern but tbe levels were relatively lower.

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Symbiotic Interactions

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The Ecology of Cyanobacteria

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Occurrence and Localization of Phycoerythrin in Symbiotic Nostoc of Cycas revoluta and in the Free-Living Isolated Nostoc 7422

Cited by 15 publications

References 18 publications

The Nostoc-Gunnera magellanica symbiosis: Phycobiliproteins, carboxysomes and Rubisco in the cyanobiont

The Nostoc-Gunnera magellanica symbiosis: Phycobiliproteins, carboxysomes and Rubisco in the cyanobiont

The marine lichen Lichina confinis (O. F. Müll.) C. Ag.: ultrastructure and localization of nitrogenase, glutamine synthetase, phycoerythrin and ribulose 1, 5‐bisphosphate carboxylase/oxygenase in the cyanobiont

Symbiotic Interactions

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