Occurrence and identification of a phytoplasma associated with Pinus brutia witches’ broom disease in Isfahan, Iran

Abstract

Your article is protected by copyright and all rights are held exclusively by Australasian Plant Pathology Society Inc.. This e-offprint is for personal use only and shall not be selfarchived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".

Molecular detection and identification of phytoplasmas associated with Catharanthus roseus, Pinus eldarica, and Petunia hybrida in southeastern Iran's urban green spaces

“…The ball‐shaped shoot proliferations on phytoplasma‐affected pine trees in urban green spaces of Kerman county were similar to those reported on affected pine trees in the Isfahan province (Babaei, Esmaeilzadeh‐Hosseini, Zandian, & Bertaccini, 2020). However, ball or disc‐like vegetative structures were found on the trunks of phytoplasma‐infected pine trees (Figure 2g,h), which appear to be unique.…”

Identification of a 16SrIX‐B phytoplasma strain associated with Daphne mucronata phyllody in Iran

“…Total DNA was extracted from 0.3 g midrib tissue of fresh leaves using the procedure of Zhang et al (1998). Total DNA extracted from a Ziziphus jujube plant infected with a phytoplasma strain 16SrVI‐A (Babaei et al, 2020) was used as positive control and a DNA sample from a healthy almond plant grown in the greenhouse was used as negative control. DNA samples were subjected to nested PCR, using primers P1/P7 (amplifying 16SrRNA gene, 16S‐23S spacer region and the 5′ end of the 23S rRNA gene) (Deng & Hiruki, 1991; Schneider et al, 1995) for direct amplification.…”