Occurrence and genetic diversity of Blastocystis in Korean cattle

Lee, Haeseung; Lee, Seung-Hun; Seo, Min-Goo; Kim, Ha-Young; Kim, Jong Wan; Lee, Yu-Ran; Kwon, Oh-Deog; Kwak, Dongmi

doi:10.1016/j.vetpar.2018.06.010

Cited by 46 publications

(47 citation statements)

References 22 publications

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“…has also been reported at prevalence rates ranging from 0 to 7.5% in captive (Alfellani et al 2013b;Cian et al 2017) and freeliving (Betts et al 2018) carnivore species, and from 0 to 2.6% in domestic dogs (Abe et al 2002;Moura et al 2018;Paulos et al 2018) in different geographical areas of the world. In contrast, the occurrence of the parasite has been identified at comparatively higher rates (6.0-54.1%) in domestic ruminants, particularly cattle and sheep (Lee et al 2018;Li et al 2018;Masuda et al 2018). Taken together, these findings seem to indicate that Blastocystis colonization/infection is less common in strict carnivore species than in ruminants.…”

Section: Resultsmentioning

confidence: 82%

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Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain

Calero‐Bernal

Santı́n

Maloney

et al. 2019

J Eukaryotic Microbiology

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The occurrence and molecular diversity of the stramenopile eukaryote Blastocystis sp. was investigated by PCR and sequencing (Sanger and NGS) methods in 380 faecal specimens of free‐living carnivores in Spain. Blastocystis sp. was confirmed in 1.6% (6/380) of the specimens analysed. Two samples from a common genet and a fox were successfully subtyped as ST7 by Sanger. Using NGS, ST14 was found in a fox and a European polecat, ST7 in a fox, and two additional foxes presented mixed infections of ST1/ST2/ST4 and ST1/ST2/ST7, respectively. Wild carnivore species could act as carriers of zoonotic Blastocystis subtypes.

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Section: Resultsmentioning

confidence: 82%

“…In contrast, the occurrence of the parasite has been identified at comparatively higher rates (6.0–54.1%) in domestic ruminants, particularly cattle and sheep (Lee et al. ; Li et al. ; Masuda et al.…”

Section: Resultsmentioning

confidence: 99%

Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain

Calero‐Bernal

Santı́n

Maloney

et al. 2019

J Eukaryotic Microbiology

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“…A total of 254 single dairy cattle samples collected from 37 villages and 55 farms located in two governorates of North Lebanon were screened in this study (Table 1) (Masuda et al, 2018), Thailand (50.0%) (Udonsom et al, 2018), Colombia (80.0%) (Ramirez et al, 2014) and Libya (41.7%) (Alfellani et al, 2013a) but was significantly higher than that reported in Korea (6.7%) (Lee et al, 2018), China (9.5% and 10.3%) (Zhu et al, 2017;Wang et al, 2018, respectively), Iran (9.6%) (Badparva et al, 2015), the United Kingdom (22.6%) (Alfellani et al, 2013a) and the United States (19.1% and 2.9%) (Fayer et al, 2012;Maloney et al, 2019, respectively).…”

Section: Resultsmentioning

confidence: 89%

“…in other animal groups, such as bovids and particularly livestock cattle. In the main recent molecular epidemiological surveys including a large number of specimens (Zhu et al, 2017;Lee et al, 2018;Masuda et al, 2018;Wang et al, 2018;Maloney et al, 2019), the prevalence of the parasite in domestic dairy/beef cattle varied between 2.9% and 54.1%, depending on the country. The potential significant occurrence of Blastocystis sp.…”

Section: Introductionmentioning

confidence: 99%

First report on the prevalence and subtype distribution of Blastocystis sp. in dairy cattle in Lebanon and assessment of zoonotic transmission

et al. 2019

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Blastocystis sp. is frequently identified in a wide range of animal hosts, including bovids. Because of its burden and zoonotic potential, this parasite has been sought in domestic cattle from various countries, since this livestock may also represent a possible reservoir of human infection. However, epidemiological data regarding the prevalence and ST distribution of Blastocystis sp. in this animal group is lacking in Lebanon. Therefore, faecal samples were collected from a total of 254 dairy cattle raised on 55 farms located in the North Lebanon region and screened for the presence of the parasite by quantitative realtime PCR. The overall prevalence of Blastocystis sp. was shown to reach 63.4% in cattle livestock. Sequence analysis of positive samples indicated the presence of seven STs, with predominance of ST10 (44.0%) and ST14 (36.8%) and lower proportions of ST2 (8.0%), ST1 (7.2%), ST5 (2.4%), ST3 and ST7 (0.8% each). This survey was the first conducted worldwide reporting ST2 and ST7 in domestic cattle and confirmed that ST10 and ST14 represent cattle-adapted STs in view of their high prevalence. Faecal samples from in-contact dairy farmers and patients hospitalised in the same Lebanese governorate who reported no contact with cattle livestock were also analysed for the presence of Blastocystis sp. The same three STs were identified in both human cohorts, with predominance of ST3, followed either by ST1 or ST2 depending of the group. No other STs, including ST10 or ST14, have been reported. Moreover, even though ST1, ST2 and ST3 were found to be common to dairy cattle and farmers cohorts, only one ST3 isolate showed 100% sequence identity between both hosts. Consequently, the presence and low prevalence of ST1, ST2, ST3, ST5 and ST7 identified herein in domestic cattle, most of which exhibit low host specificity, could be derived from occasional direct exposure to faecal material from human and non-human hosts or by ingestion of contaminated drinking water or food in the enclosure of the farms. 3 Together with the absence of ST10 and ST14 in the human population, these data suggest that cattle play a negligible role as zoonotic reservoirs of Blastocystis sp.

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“…(CH-II-7 IC, CH-II-7 IE, CH-II-8 IC, CH-II-1 SIE, PV-IV-3 IC, PV-V-9 IC, SS-III-1 IC) exhibited 100% identity with each other and corresponded to isolates identified in three different fish species (herring, saithe and mackerel) caught in two different geographical areas (Eastern English Channel and Northern North Sea). These sequences showed only 92% identity with those of ST10 isolates found in Artiodactyla and mainly bovid, which are considered as natural hosts of this ST [16,22,24,37,38], but were identical to that of the untypable isolate ZLC7 identified in a reptile (Boa constrictor) housed in the zoo of Lille in France [16]. As shown in our phylogenetic tree, these 7 sequences emerged at the basis of a large group including, more specifically, the reptilian cluster NMAST II with BP and BPP of 50% and 0.90 respectively, and formed a sister group with reptilian isolates belonging to NMAST III.…”

Section: St Distribution Of Blastocystis Sp In Edible Marine Fishmentioning

confidence: 93%

First Report on the Prevalence and Subtype Distribution of Blastocystis sp. in Edible Marine Fish and Marine Mammals: A Large Scale-Study Conducted in Atlantic Northeast and on the Coasts of Northern France

et al. 2020

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Blastocystis is frequently identified in humans and animal hosts and exhibits a large genetic diversity with the identification of 17 subtypes (STs). Despite its zoonotic potential, its prevalence and ST distribution in edible marine fish and marine mammals remain unknown. A large-scale survey was thus conducted by screening 345 fish caught in Atlantic Northeast and 29 marine mammals stranded on the coasts of northern France for the presence of the parasite using real-time Polymerase Chain Reaction PCR. The prevalence of the parasite was about 3.5% in marine fish. These animals were mostly colonized by poikilotherm-derived isolates not identified in humans and corresponding to potential new STs, indicating that fish are natural hosts of Blastocystis. Marine fishes are also carriers of human STs and represent a likely limited source of zoonotic transmission. 13.8% of the marine mammals tested were colonized and 6 different STs were identified including 3 potential new STs. The risk of zoonotic transmission through marine mammals is insignificant due to the lack of repeated contact with humans. The present survey represents the first data regarding the prevalence and ST distribution of Blastocystis in marine fish and marine mammals and provides new insights into its genetic diversity, host range and transmission.

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Occurrence and genetic diversity of Blastocystis in Korean cattle

Cited by 46 publications

References 22 publications

Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain

Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain

First report on the prevalence and subtype distribution of Blastocystis sp. in dairy cattle in Lebanon and assessment of zoonotic transmission

First Report on the Prevalence and Subtype Distribution of Blastocystis sp. in Edible Marine Fish and Marine Mammals: A Large Scale-Study Conducted in Atlantic Northeast and on the Coasts of Northern France

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