Occurrence and diversity of Pichia spp. in marine environments

Li, Jing; Chi, Zhen‐Ming; Wang, Xianghong; Wang, Lin; Sheng, Jun; Gong, Fang

doi:10.1007/s11802-008-0281-0

Cited by 5 publications

(2 citation statements)

References 20 publications

(21 reference statements)

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“…Previously, P. annomala was reported as a minor occasional component in the gut of rainbow trout (Gatesoupe, 2007). Li et al (2008b) also documented the presence of Pichia spp. in the gut of marine fishes.…”

Section: Discussionsupporting

confidence: 58%

Isolation of tannase-producing microbiota from the gastrointestinal tracts of some freshwater fish

Mandal

Ghosh

2012

Journal of Applied Ichthyology

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Summary Tannins are the most abundant among the plant‐derived antinutrients that bind readily with protein and other macromolecules to form indigestible complexes, thereby reducing the nutritional value of the plant feedstuffs. Presence of tannase‐producing gut microbiota in herbivorous animals has been suggested to overcome the antinutritional effects of tannins. However, this topic has been less investigated in herbivorous/omnivorous fish species. The present study was undertaken to evaluate the presence of tannase‐producing autochthonous microbiota in the gastrointestinal (GI) tracts of some culturable freshwater teleosts and to identify most promising tannase‐producing strains by molecular methods. Isolation and enumeration of tannase‐producing autochthonous microbiota have been carried out in the gut of ten culturable freshwater teleosts, namely catla (Catla catla), silver carp (Hypophthalmichthys molitrix), rohu (Labeo rohita), grass carp (Ctenopharyngodon idella), mrigal (Cirrhinus mrigala), common carp (Cyprinus carpio), bata (Labeo bata), kalbasu (Labeo calbasu), tilapia (Oreochromis mossambicus), and Nile tilapia (Oreochromis niloticus). Culturable heterotrophic and tannase‐producing microbial populations evaluated on tryptone soya agar and selective tannic acid agar media, respectively, revealed the maximum in the hindguts of all fish species studied. Out of 72 tannase‐producing colonies, 18 randomly selected isolates were maintained as pure cultures and evaluated quantitatively for tannase production. Among these, four most promising tannase producers were identified by 16S/26S rDNA sequencing following nucleotide blast and deposited in the National Centre for Biotechnology Information (NCBI) GenBank. The strain LR01 isolated from rohu was a bacterium, Enterobacter asburae (GenBank Accession No. ). However, the strains CM02, OM01 and LR03 isolated from mrigal, tilapia and rohu were yeasts and identified as Pichia kudriavzevii (GenBank Accession No. ), Candida tropicalis (GenBank Accession No. ) and Candida parapsilosis (GenBank Accession No. ), respectively. To the authors' knowledge, the present study is the first to report tannase‐producing autochthonous microbiota in the gut of freshwater teleosts. Tannin‐degrading microbiota detected in the present study may endow the fish with some ecological advantages by enabling them to overcome the anti‐nutritional effects of plant tannins.

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Section: Discussionsupporting

confidence: 58%

Isolation of tannase-producing microbiota from the gastrointestinal tracts of some freshwater fish

Mandal

Ghosh

2012

Journal of Applied Ichthyology

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“…is also frequently obtained from marine environments. For example, P. guilliermondii, P. ohmeri, P. fermentans, P. burtonii and P.chia anomala were obtained from surface of Sargassum pallidum, gut of Hexagrammos otakii, gut of yellow-fin tuna, gut of Lepidorigla microptera and gut of sea squirt [17]. In this study, 7.44% of the yeast strains obtained from the mangrove ecosystems were Pichia spp.…”

Section: Isolation and Routine Identification Of The Yeast Strainsmentioning

confidence: 63%

Occurrence and Diversity of Yeasts in the Mangrove Ecosystems in Fujian, Guangdong and Hainan Provinces of China

Chi

Liu

et al. 2012

Indian J Microbiol

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Mangrove wetland is a unique ecosystem and has rich bioresources. In this article, the roots, stems, branches, leaves, barks, fruits, and flowers from 12 species of the mangrove plants and six species of the accompanying mangrove plants, seawater and sediments in mangrove ecosystems in China were used as sources for isolation of yeasts. A total of 269 yeasts strains were obtained from the samples. The results of routine identification and phylogenetic analysis showed that they belonged to 22 genera and 45 species. Of all the 269 strains, Candida spp. was predominant with the proportion of 44.61%, followed by Kluyveromyces spp. (8.55%), Pichia spp. (7.44%), Kodamaea ohmeri (5.58%), Issatchenkia spp. (4.83%) and Debaryomyces hansenii (4.46%). We also found that strains N02-2.3 and ST3-1Y3 belonged to the undescribed species of Pichia sp. and Trichosporon sp. respectively while strain HN-12 was not related to any known yeast strains. This means that different yeast strains of Candida spp. especially C. tropicalis were widely distributed in the mangrove ecosystems and may have an important role in the mangrove ecosystems. The results also showed that some of them may have potential applications.

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High level lipid production by a novel inulinase-producing yeast Pichia guilliermondii Pcla22

Wang

Chi

Song

et al. 2012

Bioresource Technology

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Occurrence and diversity of Pichia spp. in marine environments

Cited by 5 publications

References 20 publications

Isolation of tannase-producing microbiota from the gastrointestinal tracts of some freshwater fish

Isolation of tannase-producing microbiota from the gastrointestinal tracts of some freshwater fish

Occurrence and Diversity of Yeasts in the Mangrove Ecosystems in Fujian, Guangdong and Hainan Provinces of China

High level lipid production by a novel inulinase-producing yeast Pichia guilliermondii Pcla22

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