Milk and dairy products are important for health because they contain many vitamins and minerals in addition to essential nutrients such as proteins, fats, and carbohydrates. However, information on the pantothenic acid and pyridoxine contents of milk and dairy products distributed in Korea is limited. Therefore, we used high-performance liquid chromatography to determine the pantothenic acid and pyridoxine contents of these products. The pantothenic acid content range was 0.000∼0.296 mg/100 g, and that of pyridoxine was 0.000∼0.198 mg/100 g in milk. Also, the pantothenic acid and pyridoxine contents in dairy products ranged between 0.076∼5.252 mg/kg and 0.000∼0.826 mg/kg, respectively. This study provides information on the pantothenic acid and pyridoxine contents of milk and dairy products. We believe that the results of this study will contribute to improving public health.