Occurrence and comparison of the expressed keratins in cultured human fibroblasts, endothelial cells and their sarcomas

Katagata, Yohtaro; Takeda, Hikaru; Ishizawa, Toshiyuki; Hozumi, Yasukazu; Kondô, Shigeo

doi:10.1016/s0923-1811(02)00039-7

Cited by 19 publications

(13 citation statements)

References 35 publications

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“…K17 expression in HepG2 cells were also found in this study. Although keratin expression in mesenchymal cells such as endothelial cells remains controversial, many reports reveal that keratins may be expressed by certain endothelial cells, such as http://bmbreports.org BMB reports cultured endothelial cells and endothelial cells of blood vessels in synovial and submucosal tissues (16)(17)(18)(19). These data are consistent with our RT-PCR and Western blot findings showing that keratin expression is present in cultured endothelial cells.…”

Section: Discussionsupporting

confidence: 91%

“…Cell migration is regulated by a network of intrinsic factors and external stimuli, involving changes in the cytoskeleton, cell-substrate adhesions and the extracellular matrix (20). Studies by Katagata showed that keratins in cultured fibroblasts and endothelial cells, including K17, only existed as the extremely thin fiber-like substances but not as the filamentous structures (17). This finding suggests that keratin IFs in cultured fibroblasts and endothelial cells may act as signal transducers rather than mechanical components.…”

Section: Discussionmentioning

confidence: 99%

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Keratin 17 identified by proteomic analysis may be involved in tumor angiogenesis

Zhang²,

Huang³

et al. 2009

BMB Reports

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Keratin 17 identified by proteomic analysis may be involved in tumor angiogenesis

Zhang²,

Huang³

et al. 2009

BMB Reports

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“…Fibroblast and endothelial cell culture show that some keratins also have non-structural roles in intracellular signaling and vascular growth through angiogenesis (Katagata et al, 2002;Xu et al, 2009). Therefore, it may be possible that a worse radiotherapy response in patients with low K17 expression is due to less oxygen permeating tumor cells and consequently a lower production of oxygen reactive species (ROS), which are necessary for lethal damage after radiotherapy (Karar and Maity, 2009).…”

Section: Discussionmentioning

confidence: 99%

Keratins 17 and 19 expression as prognostic markers in oral squamous cell carcinoma

Coelho¹,

Peterle²,

Santos³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Five-year survival rates for oral squamous cell carcinoma (OSCC) are 30% and the mortality rate is 50%. Immunohistochemistry panels are used to evaluate proliferation, vascularization, apoptosis, HPV infection, and keratin expression, which are important markers of malignant progression. Keratins are a family of intermediate filaments predominantly expressed in epithelial cells and have an essential role in mechanical support and cytoskeleton formation, which is essential for the structural integrity and stability of the cell. In this study, we analyzed the expressions of keratins 17 and 19 (K17 and K19) by immunohistochemistry in tumoral and non-tumoral tissues from patients with OSCC. The results show that expression of these keratins is higher in tumor tissues compared to non-tumor tissues. Positive K17 expression correlates with lymph node metastasis and multivariate analysis confirmed this relationship, revealing a 6-fold increase in lymph node metastasis when K17 is expressed. We observed a correlation between K17 expression with disease-free survival and disease-specific death in patients who received surgery and radiotherapy. Multivariate analysis revealed that low expression of K17 was an independent marker for early disease relapse and disease-specific death in patients treated with surgery and radiotherapy, with an approximately 4-fold increased risk when compared to high K17 expression. Our results suggest a potential role for K17 and K19 expression profiles as tumor prognostic markers in OSCC patients.

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“…To evaluate the K index (number of K subunits expressed in each cultured cell line), numerous types of K monoclonal antibodies were used for western blot analysis. In addition, 2D-PAGE was conducted as described previously (6,14).…”

Section: Western Blot Analysismentioning

confidence: 99%

Downregulation of keratins 8, 18 and 19 influences invasiveness of human cultured squamous cell carcinoma and adenocarcinoma cells

Uchiumi

Yamashita

Katagata

2011

Experimental and Therapeutic Medicine

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Abstract. Keratin (K) expression index has been reported to be related to cell invasion activity in adenocarcinoma. In a previous study, we observed a negative correlation between K expression and cell invasion activity; i.e., when many Ks are expressed in the cells, the cell activity is low. To further elucidate the correlation between Ks and invasion activity, RNA interference experiments of K8, K18 and K19 were carried out to clarify the essential role of Ks using T24 and HEC-1 as typical squamous cell carcinoma and adenocarcinoma cells, respectively. K8 small interfering RNA (siRNA) was most effective against K18 and K19 expression and demonstrated the strongest effect on relative invasion activity among the siRNAs used. These results suggest that K8/K18 or K8/K19 filaments may play roles in internal cell structure and invasion activity. Moreover, K18 and K19 were capable of substituting for each other, and K18 or K19 formed filaments with K8. In addition, cells treated with K8 siRNA demonstrated high invasion activity, which was approximately double that observed with control siRNA in HEC-1 cells. The order of effects was K8>K19>K18 in the two cell lines. The above results suggest that K8 may play a signifiant role in invasive functions in epithelial and metastatic cells. IntroductionAs inferred from the developmental origins of epithelial tissues, keratins (Ks) may be expressed in adenocarcinoma and urinary bladder carcinoma, as well as in squamous cell carcinoma (SCC). In fact, 28 type I Ks and 26 type II Ks as components of intermediate filaments (IFs) are known to be expressed in human epithelial cells (appendage-forming epithelia such as inner hair follicles) and structural epithelial cells (hard structures and appendages such as hair, nail and tongue). Moreover, Ks are differentially expressed as specific pairs of type I (smaller and relatively acidic, 40-63 kDa and pI 4.5-5.5) and type II (larger and more basic, 53-67 kDa and pI 4.5-7.5) proteins, both of which are necessary for filament formation: at least one pair of Ks is always expressed in every epithelial cell (1-3).The type-specific distribution of IF protein has been increasingly used as the basis for the differential histopathological diagnosis of tumors where morphological criteria have proven to be inconclusive. It has been reported that Ks are expressed in epidermal melanocytes derived from neural crest cells and neural ectoderm (4,5). The evidence presented above suggests that K expression may be related to further differentiation in epithelial tissues, and is not derived from original cells or tissues.In a previous study, we observed a negative correlation between K expression (the number of Ks expressed) and cell invasion capability in three cultured human adenocarcinoma cell lines (6). The number of expressed Ks increases inversely with the invasive index as follows: SW-13 (7) (K number, 2.5; invasion index, 1.781), RERF-LC-OK (K number, 6.5; invasion index, 1.219), and HEC-1 (8) (K number, 7.5; invasion index, 0.562). That is, the nu...

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Occurrence and comparison of the expressed keratins in cultured human fibroblasts, endothelial cells and their sarcomas

Cited by 19 publications

References 35 publications

Keratin 17 identified by proteomic analysis may be involved in tumor angiogenesis

Keratin 17 identified by proteomic analysis may be involved in tumor angiogenesis

Keratins 17 and 19 expression as prognostic markers in oral squamous cell carcinoma

Downregulation of keratins 8, 18 and 19 influences invasiveness of human cultured squamous cell carcinoma and adenocarcinoma cells

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