Occurrence and Characterization of mcr-1-Positive Escherichia coli Isolated From Food-Producing Animals in Poland, 2011–2016

Zając, Magdalena; Sztromwasser, Paweł; Bortolaia, Valeria; Leekitcharoenphon, Pimlapas; Cavaco, Lina; Ziętek-Barszcz, Anna; Hendriksen, René S.; Wasyl, Dariusz

doi:10.3389/fmicb.2019.01753

Cited by 66 publications

(62 citation statements)

References 60 publications

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“…These findings are consistent with a German study where the prevalence of E. coli with the mcr-1 gene was higher in turkey (8.4%) than in chicken meat (4.3%) (Irrgang et al, 2016). Furthermore, this is in accordance with the higher observed prevalence of mcr-1-positive bacteria in turkeys from Poland, Germany, France, and Italy in comparison with broilers (Irrgang et al, 2016;Perrin-Guyomard et al, 2016;Alba et al, 2018;Zajac et al, 2019).…”

Section: Discussionsupporting

confidence: 92%

Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products

et al. 2019

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The global food chain may significantly promote the dissemination of bacteria resistant to antibiotics around the world. This study was aimed at determining the prevalence and genetic characteristics of Enterobacteriaceae with mcr-mediated colistin (CT) resistance in retail meat of different origins. Bacteria of the Enterobacteriaceae family carrying the mcr-1 gene were detected in 21% (18/86) of the examined samples, especially in turkey meat and liver originating from EU and non-EU countries (19%) and in rabbit meat imported from China (2%). The examined samples of the meat and liver of chicken and other poultry and of pork and beef were negative for the presence of bacteria carrying the mcr-1 to mcr-5 genes. A huge number of isolates belonging to Escherchia coli (n = 54), Klebsiella pneumoniae (n = 6), and Citrobacter braakii (n = 1) carrying the mcr-1 gene were obtained. Despite the high heterogeneity of the tested isolates, the mcr-1 gene was localized on only three types of plasmids (IncX4, IncHI2, and IncI2). The most frequent type of plasmid was IncX4, which carried the mcr-1 gene in 77% of E. coli and K. pneumoniae isolates from turkey meat and liver samples from the Czechia, Germany, Poland, and Brazil. Our findings indicate highly probable interspecies transfer of IncX4 and IncI2 plasmids within one meat sample. The co-resistance of plasmid-mediated CT resistance encoded by the mcr-1 and ESBL genes was detected in 18% of the isolates. Another noteworthy finding was the fosA3 gene coding for fosfomycin resistance in a multidrug-resistant isolate of E. coli from rabbit meat imported from China. The observed high level of Enterobacteriaceae with plasmids carrying the mcr-1 gene in retail meat reflects the need for Europe-wide monitoring of mcr-mediated CT resistance throughout the whole food chain.

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Section: Discussionsupporting

confidence: 92%

Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products

et al. 2019

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“…The mcr-1 flanking areas described herein have also been reported in studies elsewhere for the three-replicon families (Hadjadj et al, 2017;Zurfluh et al, 2017;Duggett et al, 2018;Zając et al, 2019). The ISApl1 flanking the mcr-1 gene appears to be a very efficient mechanism for "traveling" around, facilitating mcr-1 transposition between a limited number of incompatibility families of plasmids (Matamoros et al, 2017).…”

Section: Discussionsupporting

confidence: 68%

“…Plasmid mediated colistin resistance has been described worldwide in a variety of Enterobacterales of different origins, environment, food producing animals, wildlife and humans (Zurfluh et al, 2016;Guenther et al, 2017;Ovejero et al, 2017;Barlaam et al, 2019;Elbediwi et al, 2019;Lalaoui et al, 2019;Mendes Oliveira et al, 2019;Nang et al, 2019;Zając et al, 2019). In particular, in Spain, mcr-1 has been described circulating in pig farms for many years (Quesada et al, 2016;Garcia-Menino et al, 2018), and our results demonstrate its presence in pig farms in Spain as early as 2005.…”

Section: Discussionsupporting

confidence: 54%

mcr-Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in Spain

et al. 2020

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Colistin has become the last-line antimicrobial for the treatment of multidrug resistant (MDR) Enterobacterales in human medicine. To date, several colistin resistance genes have been described. Of them mcr-1 is disseminated worldwide in Escherichia coli of human and animal origin. The aim of this study was to characterize mcr-mediated resistance plasmids from E. coli of animal origin in Spain. From our strain collection, 70 E. coli of pig origin collected between 2005 and 2014 (10 per year, except for years 2009-2010-2013) were randomly selected and screened for the presence of mcr-genes. Additionally, 20 E. coli isolated in 2011 from white storks (Ciconia ciconia) from the same urban household waste landfill associated colony were also included. Whole genome sequencing of mcr-positive isolates was carried out on a MiSeq (Illumina). Hybrid whole genome sequencing strategy combining nanopore and Illumina technologies were performed in a selection of isolates to close the genomes and plasmids and identify the presence of antimicrobial resistance genes. Minimum inhibitory concentration (MIC) was used to assess the susceptibility to colistin. Mating experiments were carried out to evaluate transferability of the mcr-genes. A total of 19 mcr-1 and one mcr-4 positive isolates were detected, 15 from pigs distributed during the study period, and five from storks collected in 2011. No other mcr-variants were found. The MICs for colistin ranged between 4 and >4 mg/L. High diversity of STs were detected among the mcr-1 positive E. coli isolates, with only ST-10 shared between pigs and white storks. Except for one isolate, all were genotypic and phenotypically MDR, and five of them also harbored cephalosporin resistance genes (bla CTX−M−14 , bla SHV−12 , and three bla CMY−2). mcr-1 genes were mobilizable by conjugation, associated with IncX4, IncHI2, and IncI2 plasmids. In our study, mcr-1 genes have been circulating in pig farms since 2005 harbored by a variety of E. coli clones. Its persistence may be driven by co-selection since plasmids containing mcr-1 also exhibit resistance to

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“…The other two A-E. coli isolates were assigned to ST665 (A 0 ) and ST48 (A 1 ), the latter belonging to the clonal complex 10. These clones have been sporadically identified as CMY-2 producers from clinical (ST665 in South Africa) or non-clinical (ST48 in Tunisia and Poland) settings [9][10][11], or associated with other mechanisms of antibiotic resistance (e.g., ESBL/carbapenemase production or plasmid-mediated colistin resistance) in different reservoirs (hospitals, animals or food products) [11,12], further supporting circulation of these clones along the food chain. All the CMY-2-producing E. coli isolates were multidrug-resistant (non-susceptible to at least one agent in three or more antimicrobial categories tested).…”

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confidence: 97%

Acquired AmpC β-Lactamases among Enterobacteriaceae from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal

et al. 2020

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We aimed to investigate the occurrence of acquired AmpC β-lactamases (qAmpC), and characterize qAmpC-producing Enterobacteriaceae from different non-clinical environments in Portugal. We analysed 880 Enterobacteriaceae resistant to third-generation cephalosporins recovered from 632 non-clinical samples [healthy human and healthy animal (swine, chickens) faeces; uncooked chicken carcasses; aquatic and trout aquaculture samples]. Bacterial and qAmpC identification, antibiotic susceptibility, clonal (PFGE, MLST) and plasmid (S1-/I-CeuI-PFGE, replicon typing, hybridization) analysis were performed using standard methods. The occurrence of qAmpC among Enterobacteriaceae from non-clinical origins was low (0.6%; n = 4/628 samples), corresponding to CMY-2-producing Escherichia coli from three healthy humans (HH) and one uncooked chicken carcass (UCC). We highlight a slight increase in CMY-2 human faecal carriage in the two periods sampled [1.0% in 2013-2014 versus 0% in [2001][2002][2003][2004], which is in accordance with the trend observed in other European countries. CMY-2-producing E. coli belonged to B2 2 -ST4953 (n = 2, HH), A 0 -ST665 (n = 1, HH) or A 1 -ST48 (n = 1, UCC) clones. bla CMY-2 was identified in non-typeable and IncA/C 2 plasmids. This study is one of the few providing an integrated evaluation of the qAmpC-producing Enterobacteriaceae occurrence, which was low, from a very large collection of different non-clinical origins. Further surveillance in contemporary collections can provide an integrated epidemiological information of potential shifts in reservoirs, transmission routes and mechanisms of dissemination of bla qAmpC in non-clinical settings.

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Occurrence and Characterization of mcr-1-Positive Escherichia coli Isolated From Food-Producing Animals in Poland, 2011–2016

Cited by 66 publications

References 60 publications

Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products

Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products

mcr-Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in Spain

Acquired AmpC β-Lactamases among Enterobacteriaceae from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal

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