Occurrence and antimicrobial resistance pattern of E. coli O157:H7 isolated from foods of Bovine origin in Dessie and Kombolcha towns, Ethiopia

Abstract

E. coli are frequently isolated food-borne pathogens from meat, milk, and their products. Moreover, there has been a significant rise in the antimicrobial resistance patterns of E. coli O157:H7 to commonly used antibiotics. A cross-sectional study was conducted from October 2019 to July 2021 to estimate prevalence and identify associated factors of E. coli and E. coli O157:H7 and to determine antibiotic resistance pattern of E. coli O157:H7 from foods of bovine origin in Dessie and Kombolcha towns. A total of 384 samples were collected. Systematic and simple random sampling techniques were employed for sampling carcasses and milking cows, respectively. E. coli and E. coli O157:H7 were detected according to recommended bacteriological protocols. E. coli O157:H7 strains were evaluated for in vitro antimicrobial susceptibility using agar disk diffusion method. Both descriptive and inferential statistical techniques were applied to analyze the data. Overall prevalence rates of E. coli and E. coli O157:H7 were 54.7% and 6.5%, respectively. Highest prevalence rates of E. coli (79.6%) and E. coli O157:H7 (16.7%) were obtained from carcass swabs and milk tank samples, respectively. Unlike E. coli O157:H7, a statistically significant difference in the E. coli prevalence (P<0.05) was observed among the different sample types. Multidrug resistance was observed among all isolates of E. coli O157:H7. All E. coli O157:H7 isolates (100.0%) were susceptible to Ampicillin, Sulfamethoxazole-trimethoprim, and Norfloxacin. On the contrary, all of the isolates (100%) were resistant to Penicillin G, Vancomycin, and Oxacillin. The current study indicated that different foods of bovine origin in the study area were unsafe for human consumption. Hence, good hygienic production methods should be employed to ensure the safety of foods of bovine origin.

Escherichia coli isolates from vegetable farms in Addis Ababa, Ethiopia: Antimicrobial susceptibility profile and associated resistance genetic markers

“…In addition, the survival of E. coli on manure‐amended agricultural farms could be affected by the soil management system of the farm (Franz et al., 2005 ). In this study, no E. coli O157:H7 was detected, but previous studies reported E. coli O157:H7 in foods of animal origin in Ethiopia (Abebe et al., 2023 ; Hamid et al., 2018 ). The common source of contamination of food of animal origin with E. coli O157:H7 is the fresh feces of cattle (Ferens & Hovde, 2010 ).…”

Seasonal variation of Salmonella enterica prevalence in milk and cottage cheese along the dairy value chain in three regions of Ethiopia

“…While Senbetu ( 2014 ) and Habtamu et al ( 2018 ) from Hawassa reported a higher prevalence rate of S. enterica , 25%, and 17.8%, respectively. Among the S.enterica prevalence reports from the Amhara region, Abebe et al ( 2023 ) (Dessie and Kombolcha), Mulaw ( 2017 ), and Hailu et al ( 2015 ) (Gondor) reported 5.92%, 9.35%, and 3.24%, respectively, which is lower than the S.enterica prevalence report during the wet season.…”

Antibiotic residues and microbial contamination in pasteurized whole milk intended for human consumption

“…Contamination of human milk consumption is one of the biggest concerns worldwide because of the presence of microbial and antibiotic residues in milk [5]. Milk may contain contaminants, such as pathogenic bacteria such as Listeria monocytogenes, Salmonella spp., Escherichia coli, and Staphylococcus aureus, as well as residues of antibiotics, pesticides, and other chemicals, which can be harmful to human health [6]. Penicillin, tetracycline, sulfonamides, cephalosporins, and streptomycin, which can be a risk to human health due to the development of antibiotic resistance [7], are the main antibiotics found in milk.…”

“…Penicillin, tetracycline, sulfonamides, cephalosporins, and streptomycin, which can be a risk to human health due to the development of antibiotic resistance [7], are the main antibiotics found in milk. Residues and contaminants in dairy products for final consumption have previously been reported in other countries [6][7][8][9][10][11][12][13]. The National Institute for Food and Drug Surveillance (INVIMA) of the Colombian Government regulates and controls drugs and other food contaminants in Colombia.…”