Occupational exposure to pesticides is associated with differential DNA methylation

Plaat, Diana A van der; Jong, Kim de; Vries, Maaike de; Diemen, Cleo C. van; Nedeljković, Ivana; Kromhout, Hans; Vermeulen, Roel; Postma, Dirkje S.; Duijn, Cornelia M. van; Boezen, H. Marike; Vonk, Judith M.

doi:10.1136/oemed-2017-104787

Cited by 68 publications

(52 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…DNA methylation levels in whole blood were determined with the Illumina Infinium Human Methylation 450 K array. After quality control, the final dataset contained DNA methylation data presented as beta values for 420,938 CpG probes [ 18 ] (See supplementary methods for sample processing and quality control).…”

Section: Methodsmentioning

confidence: 99%

From blood to lung tissue: effect of cigarette smoke on DNA methylation and lung function

et al. 2018

Self Cite

View full text Add to dashboard Cite

BackgroundGenetic and environmental factors play a role in the development of COPD. The epigenome, and more specifically DNA methylation, is recognized as important link between these factors. We postulate that DNA methylation is one of the routes by which cigarette smoke influences the development of COPD. In this study, we aim to identify CpG-sites that are associated with cigarette smoke exposure and lung function levels in whole blood and validate these CpG-sites in lung tissue.MethodsThe association between pack years and DNA methylation was studied genome-wide in 658 current smokers with >5 pack years using robust linear regression analysis. Using mediation analysis, we subsequently selected the CpG-sites that were also associated with lung function levels. Significant CpG-sites were validated in lung tissue with pyrosequencing and expression quantitative trait methylation (eQTM) analysis was performed to investigate the association between DNA methylation and gene expression.Results15 CpG-sites were significantly associated with pack years and 10 of these were additionally associated with lung function levels. We validated 5 CpG-sites in lung tissue and found several associations between DNA methylation and gene expression.ConclusionThis study is the first to validate a panel of CpG-sites that are associated with cigarette smoking and lung function levels in whole blood in the tissue of interest: lung tissue.Electronic supplementary materialThe online version of this article (10.1186/s12931-018-0904-y) contains supplementary material, which is available to authorized users.

show abstract

Section: Methodsmentioning

confidence: 99%

From blood to lung tissue: effect of cigarette smoke on DNA methylation and lung function

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Other data showing changes in DNA methylation patterns related to occupational exposure were reported for firefighters [ 55 ], or subjects exposed to pesticides [ 56 , 57 ], or metals [ 58 ]. A general summary with more evidence of effects of environmental chemicals on epigenetic regulation in various studies (human, animal, and in vitro) including perspectives for the future of the field of toxico-epigenomics were recently published by the epigenetic group of International Agency for Research on Cancer (IARC) [ 59 ].…”

Section: Epigenetic Aspects Of Human Adaptation To Environmental Pmentioning

confidence: 99%

The Molecular Mechanisms of Adaptive Response Related to Environmental Stress

Rössnerová

Izzotti

Pulliero

et al. 2020

IJMS

View full text Add to dashboard Cite

The exposure of living organisms to environmental stress triggers defensive responses resulting in the activation of protective processes. Whenever the exposure occurs at low doses, defensive effects overwhelm the adverse effects of the exposure; this adaptive situation is referred to as “hormesis”. Environmental, physical, and nutritional hormetins lead to the stimulation and strengthening of the maintenance and repair systems in cells and tissues. Exercise, heat, and irradiation are examples of physical hormetins, which activate heat shock-, DNA repair-, and anti-oxidative-stress responses. The health promoting effect of many bio-actives in fruits and vegetables can be seen as the effect of mildly toxic compounds triggering this adaptive stimulus. Numerous studies indicate that living organisms possess the ability to adapt to adverse environmental conditions, as exemplified by the fact that DNA damage and gene expression profiling in populations living in the environment with high levels of air pollution do not correspond to the concentrations of pollutants. The molecular mechanisms of the hormetic response include modulation of (a) transcription factor Nrf2 activating the synthesis of glutathione and the subsequent protection of the cell; (b) DNA methylation; and (c) microRNA. These findings provide evidence that hormesis is a toxicological event, occurring at low exposure doses to environmental stressors, having the benefit for the maintenance of a healthy status.

show abstract

“…Occupational exposure to pesticides has been associated with several health outcomes including cancer,1 2 neurological diseases,3 4 mental disorders,2 rheumatoid arthritis,5 respiratory diseases,6–8 various genetic biomarkers9 10 and reproductive and developmental disorders 3 11. Occupationally exposed populations comprise for example, agricultural workers, gardeners, flower growers, forestry workers and hygiene and pest control workers 12.…”

Section: Introductionmentioning

confidence: 99%

Systematic review of methods used to assess exposure to pesticides in occupational epidemiology studies, 1993–2017

et al. 2020

Self Cite

View full text Add to dashboard Cite

ObjectiveNumerous exposure assessment methods (EAM) exist for investigating health effects of occupational exposure to pesticides. Direct (eg, biomonitoring) and indirect methods (eg, self-reported exposures) are however associated with degrees of exposure misclassification. We systematically reviewed EAM in studies of occupational pesticide exposure.MethodsWe searched for articles reporting observational epidemiological studies in MEDLINE and Embase published 1993 to 2017. The relative frequency of EAM was analysed according to EAM type (direct and indirect methods), health outcome, study design, study location (country) and specificity of assessment. Temporal trends in EAM were analysed.ResultsIn 1298 included articles 1521 EAM occurrences were documented. Indirect EAM (78.3%), primarily self-reported exposures (39.3%) and job titles assessments (9.5%), were mainly applied in case-control studies (95.0%), in high-income countries (85.0%) and in studies of doctor-diagnosed health outcomes (>85%). Direct EAM (20.8%), primarily biomonitoring of blood (15.6%) or urine (4.7%), were predominantly applied in cross-sectional studies (29.8%), in lower middle-income countries (40.9%) and in studies of neurological (50.0%) outcomes. Between 1993 to 2017 no distinct time trends regarding the ratio indirect to direct methods was seen. Within the category of indirect methods use of self-reported exposures and job exposure matrices increased while assessments by job titles and registers decreased. The use of algorithms showed no trend. The specificity of pesticide assessment increased since studies assessing exposure by using job title as a proxy declined. Assessments of type of pesticide increased.ConclusionOver the last 25 years, the ratio (5:1) of indirect to direct EAM applied in articles on occupational pesticide epidemiology stayed relatively constant; changes were mainly attributable to increasing use of self-reported exposures and job exposure matrices. This review, combined with studies assessing EAM validity, will inform on magnitudes of exposure misclassification and help improve the quality of studies on occupational pesticides exposure.

show abstract

Occupational exposure to pesticides is associated with differential DNA methylation

Cited by 68 publications

References 44 publications

From blood to lung tissue: effect of cigarette smoke on DNA methylation and lung function

From blood to lung tissue: effect of cigarette smoke on DNA methylation and lung function

The Molecular Mechanisms of Adaptive Response Related to Environmental Stress

Systematic review of methods used to assess exposure to pesticides in occupational epidemiology studies, 1993–2017

Contact Info

Product

Resources

About