Occupational exposure in lead and zinc mines induces oxidative stress in miners lymphocytes: Role of mitochondrial/lysosomal damage

Abstract

The purpose of this research was to determine mitochondrial and lysosomal damage and oxidative stress status in blood lymphocytes of lead-zinc miners. This research was performed in 10 mine workers who have been in contact with lead and zinc in comparison to a control group containing 10 healthy volunteers. Lymphocytes were isolated from peripheral blood using the Ficoll standard method and then mitochondrial and lysosomal damage and oxidative stress were evaluated. The level of reactive oxygen species (ROS), collapse in the mitochondrial membrane potential (MMP) collapse, and glutathione disulfide (GSSG) content, and lysosomal damage in miners were higher than the control group. Also, viability and glutathione (GSH) content were decreased. The lymphocytes of workers of a lead-zinc mine are more susceptible to oxidative stress, mitochondrial and lysosomal damage. The proper use of safety equipment can reduce the risk of toxic agents and their subsequent hazards for mine workers.

Mitochondrial Stress by Toxic Elements - An Overview

“…An in vitro study made in RAW 264.7 murine macrophages after exposure to 50-150 ppm Cr III exhibited no mT dysfunction. A recent report from Seydi et al [94] showed that Cr VI caused Mt Membrane Potential (MMP) collapse in isolated human lymphocytes. In brief, a surge in the generation of ROS, inhibition of mT respiratory chain complex I and activation of caspase 9 mediate its effects on cell death.…”

The Effects of Zinc on Proprioceptive Sensory Function and Nerve Conduction

“…While this zinc insufficiency is concerning, however, the overuse of oral zinc supplementation is also an issue, and deaths have been reported due to consuming it in excess [4][5][6]. Additionally, zinc exposure can also stem from industrial or occupational hazards, such as the airborne zinc particulates prominent in mining operations [7,8] or the accidental contamination of food or water from storage in galvanized containers or pipes [4]. These paths of exposure could affect populations nearby, whether through chronic or acute effects, and have health repercussions that are, as of now, not fully understood.…”