Occupational asthma and IgE sensitization to cellulase in a textile industry worker

Nahm, Dong-Ho; Park, Hae‐Sim; Choi, Dong-Chull

doi:10.1016/s1081-1206(10)62593-8

Cited by 13 publications

(6 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, it is important to note that, like IgE, IgG4 is promoted by a type-2 immune response, which has been shown to foster atopic immune responses. 33,34 Since a possible association between speci®c IgG subclass and respiratory or skin allergy has also been suggested, we also tested whether serum levels of IgG subclasses are related with the magnitude of Sb exposure. Elevated serum levels of total IgG4 or allergen-speci®c IgG4 were reported for atopic dermatitis patients or individuals exposed to a chemical allergen, 35,36 and allergen-speci®c IgG1 was demonstrated in vitro to be responsible for induction of eosinophil degranulation leading to allergic in¯ammatory reactions.…”

Section: Discussionmentioning

confidence: 99%

Altered serum cytokine and immunoglobulin levels in the workers exposed to antimony

Kim

Heo

et al. 1999

Hum Exp Toxicol

View full text Add to dashboard Cite

1 Antimony (Sb), an industrial and environmental toxicant, is known to cause dermatitis and pulmonary inflammations, but the immunomodulatory effects of environmental or occupational exposure to Sb have not been reported. To initiate investigation of Sbinduced alterations of the immune system, the concentrations of the IgG subclasses, IgE, interleukin-2, interferon-g, and interleukin-4 in sera obtained from workers exposed to Sb through antimony trioxide manufacture were determined and compared with those of control subjects. 2 The serum levels of IgG1, an immunoglobulin involved in host defense against many microbial infections, were significantly lower in the sera of Sb-exposed individuals than the controls. The serum concentrations of IgE, an immunoglobulin mediating allergic hypersensitivity, also were lower in the Sb-exposed workers than the controls. In addition, the levels of interleukin-2 and interferon-g, multifunctional cytokines for T-cell mediated immunity, were lowered in the factory workers. 3 A significant positive correlation between IgG4 and urine Sb levels was found among the Sb-exposed workers, indicating a possible role of IgG4 in Sbmediated pulmonary or skin pathogenesis. 4 The present study provides the first report on immune alterations induced by occupational exposure to Sb and suggests that Sb exposure disturbs immunohomeostasis in humans observed as a function of aberrant serum cytokine and immunoglobulin levels, which could influence health.

show abstract

Section: Discussionmentioning

confidence: 99%

Altered serum cytokine and immunoglobulin levels in the workers exposed to antimony

Kim

Heo

et al. 1999

Hum Exp Toxicol

View full text Add to dashboard Cite

show abstract

“…The collected information included demographic data, complete occupational history, smoking and passive smoking, present illnesses as well as details on any respiratory, ocular, and skin symptoms. A skin prick test, using an extract of cotton fabric, was prepared according to the method described by Kim [8]. To prepare the extract from cotton fabric dust, the cotton fabric dust was soaked in phosphate-buffered saline (PBS, pH 7.4, 1:10 wt/vol) for 24 h at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Work-Related Respiratory Symptoms Among Cotton-Fabric Sewing Workers

Phakthongsuk

Sangsupawanich²,

Musigsan³

et al. 2007

International Journal of Occupational Medicine and Environmental Health

View full text Add to dashboard Cite

show abstract

“…8 Microtiter plates (Dynatech) were first coated with 100 µL/well of P chinensis (1 µg/well) extracts and left at 4°C overnight. Each well was washed 3 times with 0.05% PBS-Tween (PBST), and the remaining binding sites were blocked by incubation with 350 µL of 3% BSA-PBST for 1 hour at room temperature (RT).…”

Section: Elisa For Sige or Sigg4 Antibody To P Chinensismentioning

confidence: 99%

“…8,9 P chinensis antigen was mixed with sample buffer (1 mL of 0.5 mol/L TRIS-HCl [pH 6.8], 1.6 mL of glycerol, 1.5 mL of 10% [wt/vol] SDS, 0.4 mL of 0.5% 2-mercaptoethanol, and 3 mL of distilled water), which was then adjusted to 1 mg/mL and heated in boiling water for 5 minutes. Ten microliters of standard marker (Novex) and antigen solution were added to a Novex precast TRISglycine gel (12%) for the separation of antigens.…”

Section: Sds-page and Ige Immunoblotmentioning

confidence: 99%

Anaphylaxis caused by the new ant, Pachycondyla chinensis : Demonstration of specific IgE and IgE-binding components

Kim¹,

Park²,

Kim³

et al. 2001

Journal of Allergy and Clinical Immunology

Self Cite

View full text Add to dashboard Cite

Background: There have been no reports dealing with the pathogenic mechanism and IgE-binding components in patients with anaphylaxis caused by a sting from Pachycondyla chinensis. Objectives: This study was conducted to observe the clinical features of patients with P chinensis-induced anaphylaxis. The roles of specific (s) IgE and sIgG4 antibodies were evaluated, and IgE-binding components were identified. Methods: Seven patients with P chinensis-induced anaphylaxis and 15 unexposed control subjects were enrolled. P chinensis ants were collected at the patients' homes, and venom was prepared as P chinensis extract. Five patients complained of bee venom-induced anaphylaxis and had positive sIgE levels to yellow jacket venom, wasp venom, or both as well. Serum sIgE and sIgG4 were detected by means of ELISA. To identify IgEbinding components within P chinensis extracts, 12% SDS-PAGE with immunoblot analysis was applied. Results: All patients had positive skin prick test responses to P chinensis antigen and positive sIgE levels. Five (71%) patients had positive sIgG4 levels. Eight IgE-binding components (58, 46, 3l, 29, 27, 25, 22, and 12 kd) were noted, and the component at 12 kd was the most frequently found allergen (85%). IgE ELISA inhibition tests were performed on 2 groups of sera: one from patients with anaphylaxis induced by both P chinensis and bee venom (group A) and the other from patients with anaphylaxis induced by P chinensis venom alone without bee venom allergy (group B). ELISA inhibition tests with serum from group A showed significant inhibitions with addition of P chinensis extract, partial inhibitions with yellow jacket antigen, and minimal inhibitions with wasp or imported fire ant antigens. However, ELISA inhibition tests with serum from group B showed significant inhibitions with P chinensis antigen but no inhibition with wasp, yellow jacket, or imported fire ant antigens. Conclusions

show abstract

Occupational asthma and IgE sensitization to cellulase in a textile industry worker

Cited by 13 publications

References 14 publications

Altered serum cytokine and immunoglobulin levels in the workers exposed to antimony

Altered serum cytokine and immunoglobulin levels in the workers exposed to antimony

Work-Related Respiratory Symptoms Among Cotton-Fabric Sewing Workers

Anaphylaxis caused by the new ant, Pachycondyla chinensis : Demonstration of specific IgE and IgE-binding components

Contact Info

Product

Resources

About