Objective: The aim of this study was to determine the total mould and bacteria levels in the kindergartens in Istanbul City, Fatih District. Materials: This is a cross sectional study and was performed in 15 preschool institutions in Fatih district in May 2007. Methods: Merck Air Sampler Mas 100 was used for measurements. Results: The total amounts of bacteria grown in the stations measured (classroom, lunch room, kitchen and toilets) were as follows: 946.43 AE 1033.79 CFUÁm À3 in the morning and 849.29 AE 594 CFUÁm À3 in the afternoon. Total mould numbers were 489.64 AE 441.25 CFUÁm À3 in the morning and 993.39 AE 1013.52 CFUÁm À3 in the afternoon. Cladosporium spp. and Aspergillus spp. were found to be the most commonly reproduced mould species. The total mould levels were found to be higher in stations with aluminium window framing and floor carpeting. Furthermore, when compared in terms of total mould levels, there was no difference between concrete and brick buildings; and also, total bacteria count was higher in brick buildings during the morning measurements. Conclusion: For providing a better indoor air quality in kindergartens, natural ventilation, polyvinyl chloride windows, concrete building, minimum furniture and textile materials (sofas, cushions, curtains and kids' clothing) are preferable.